Transcript: Human XR_245712.4

PREDICTED: Homo sapiens abhydrolase domain containing 4 (ABHD4), transcript variant X2, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ABHD4 (63874)
Length:
3034
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_245712.4
NBCI Gene record:
ABHD4 (63874)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_245712.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000049928 CCGGACTTCAAACGCAAGTTT pLKO.1 756 3UTR 100% 5.625 7.875 N ABHD4 n/a
2 TRCN0000343847 CCGGACTTCAAACGCAAGTTT pLKO_005 756 3UTR 100% 5.625 7.875 N ABHD4 n/a
3 TRCN0000049929 CCAGATATGTATCCCTCCCAA pLKO.1 143 3UTR 100% 2.640 3.696 N ABHD4 n/a
4 TRCN0000049931 CACTTCTTACTCAATCAAGTA pLKO.1 465 3UTR 100% 4.950 3.465 N ABHD4 n/a
5 TRCN0000343909 CACTTCTTACTCAATCAAGTA pLKO_005 465 3UTR 100% 4.950 3.465 N ABHD4 n/a
6 TRCN0000049932 CCATTGGCTGTTCTTCGAGTA pLKO.1 616 3UTR 100% 4.050 2.835 N ABHD4 n/a
7 TRCN0000343846 CCATTGGCTGTTCTTCGAGTA pLKO_005 616 3UTR 100% 4.050 2.835 N ABHD4 n/a
8 TRCN0000049930 GCGAATTCACTTGATTCGAAA pLKO.1 905 3UTR 100% 0.000 0.000 N ABHD4 n/a
9 TRCN0000343848 GCGAATTCACTTGATTCGAAA pLKO_005 905 3UTR 100% 0.000 0.000 N ABHD4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_245712.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03902 pDONR223 100% 33.8% None 1_9delTTGTTTACT;650_729del;1116_3034del n/a
2 ccsbBroad304_03902 pLX_304 0% 33.8% V5 1_9delTTGTTTACT;650_729del;1116_3034del n/a
3 TRCN0000475126 CTATCAAGTACTGAGGACATGGTA pLX_317 2.4% 33.8% V5 1_9delTTGTTTACT;650_729del;1116_3034del n/a
4 ccsbBroadEn_03903 pDONR223 100% 33.8% None 1_9delTTGTTTACT;650_729del;1116_3034del n/a
5 ccsbBroad304_03903 pLX_304 0% 33.8% V5 1_9delTTGTTTACT;650_729del;1116_3034del n/a
6 TRCN0000476601 GGCTCACAACAACTTTAGGAACAT pLX_317 37.5% 33.8% V5 1_9delTTGTTTACT;650_729del;1116_3034del n/a
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