Transcript: Mouse XR_385167.2

PREDICTED: Mus musculus quaking (Qk), transcript variant X2, misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Qk (19317)
Length:
6614
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_385167.2
NBCI Gene record:
Qk (19317)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_385167.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000329418 TGCCAGTCATGCCTGATATTT pLKO_005 1430 3UTR 100% 15.000 21.000 N Qk n/a
2 TRCN0000233374 TAGGTGCGGTGGCTACTAAAG pLKO_005 3456 3UTR 100% 10.800 15.120 N QKI n/a
3 TRCN0000329483 TTATAACACGACCAGTCAATG pLKO_005 1495 3UTR 100% 10.800 15.120 N Qk n/a
4 TRCN0000102409 GAGCATCTAAATGAAGACTTA pLKO.1 916 3UTR 100% 4.950 3.960 N Qk n/a
5 TRCN0000329417 ATGTACAATGACACGTTAAAT pLKO_005 649 3UTR 100% 15.000 10.500 N Qk n/a
6 TRCN0000329482 CCTACAGAGACGCCAACATTA pLKO_005 1091 3UTR 100% 13.200 9.240 N Qk n/a
7 TRCN0000102406 CCTAGAGGACTTACAGCTAAA pLKO.1 796 3UTR 100% 10.800 7.560 N Qk n/a
8 TRCN0000329480 TCAATCCTTGAGTACCCTATT pLKO_005 1378 3UTR 100% 10.800 7.560 N Qk n/a
9 TRCN0000015184 CCCTACCATAATGCCTTTGAT pLKO.1 1227 3UTR 100% 5.625 3.938 N QKI n/a
10 TRCN0000015185 AGAAACTTTATGTGCCTGTAA pLKO.1 734 3UTR 100% 4.950 3.465 N QKI n/a
11 TRCN0000015187 GACGAAGAAATTAGCAGAGTA pLKO.1 619 3UTR 100% 4.950 3.465 N QKI n/a
12 TRCN0000102408 TGCCTGATATTTCAGCCCATT pLKO.1 1439 3UTR 100% 4.050 2.835 N Qk n/a
13 TRCN0000102407 GCACCAGCTACATCAATCCTT pLKO.1 1366 3UTR 100% 3.000 2.100 N Qk n/a
14 TRCN0000015183 GCTACATCAATCCTTGAGTAT pLKO.1 1372 3UTR 100% 4.950 3.465 N QKI n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_385167.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02164 pDONR223 100% 14.3% None (many diffs) n/a
2 ccsbBroad304_02164 pLX_304 0% 14.3% V5 (many diffs) n/a
3 TRCN0000467894 CCTGACTCCGTGTACCCTTCACCC pLX_317 41.9% 14.3% V5 (many diffs) n/a
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