Transcript: Mouse XR_386375.3

PREDICTED: Mus musculus PQ loop repeat containing 1 (Pqlc1), transcript variant X3, misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Pqlc1 (66943)
Length:
1239
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_386375.3
NBCI Gene record:
Pqlc1 (66943)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_386375.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000197583 CATAGTCATGATCCTTACGAT pLKO.1 650 3UTR 100% 3.000 4.200 N Pqlc1 n/a
2 TRCN0000200254 CTTCAAGACGGCCTACTTCTT pLKO.1 1190 3UTR 100% 4.950 3.465 N Pqlc1 n/a
3 TRCN0000178556 CAGAGCATAGTCATGATCCTT pLKO.1 645 3UTR 100% 3.000 2.100 N Pqlc1 n/a
4 TRCN0000156320 CTACATCACCTACCTGTCCAT pLKO.1 881 3UTR 100% 2.640 1.848 N SLC66A2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_386375.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04177 pDONR223 100% 44% None (many diffs) n/a
2 ccsbBroad304_04177 pLX_304 0% 44% V5 (many diffs) n/a
3 TRCN0000479482 GGTCTCTGCTCCGCCTTTGCGGCG pLX_317 38.9% 44% V5 (many diffs) n/a
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