Transcript: Human XR_427085.3

PREDICTED: Homo sapiens growth factor receptor bound protein 14 (GRB14), transcript variant X4, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GRB14 (2888)
Length:
1447
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_427085.3
NBCI Gene record:
GRB14 (2888)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_427085.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000303803 TGTTGTCAGCAGACCTATTTC pLKO_005 451 3UTR 100% 13.200 18.480 N GRB14 n/a
2 TRCN0000061599 CCTGAAATTCATGGTTTCTTA pLKO.1 876 3UTR 100% 5.625 3.938 N GRB14 n/a
3 TRCN0000300082 CCTGAAATTCATGGTTTCTTA pLKO_005 876 3UTR 100% 5.625 3.938 N GRB14 n/a
4 TRCN0000061602 GCGATTAGATTGCTTAAGTAT pLKO.1 1232 3UTR 100% 5.625 3.938 N GRB14 n/a
5 TRCN0000061600 GCCATCTATTCCAAACCCTTT pLKO.1 398 3UTR 100% 4.050 2.835 N GRB14 n/a
6 TRCN0000061598 CCAGAATTATATGCATCCATA pLKO.1 1267 3UTR 100% 4.950 2.970 N GRB14 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_427085.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06325 pDONR223 100% 66% None (many diffs) n/a
2 ccsbBroad304_06325 pLX_304 0% 66% V5 (many diffs) n/a
3 TRCN0000478548 GGTTAAGATAAAGCCTCCCTTCAA pLX_317 25.3% 66% V5 (many diffs) n/a
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