Construct: ORF TRCN0000478548
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006099.2_s317c1
- Derived from:
- ccsbBroadEn_06325
- DNA Barcode:
- GGTTAAGATAAAGCCTCCCTTCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRB14 (2888)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478548
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2888 | GRB14 | growth factor receptor boun... | NM_004490.3 | 99.9% | 100% | 279G>T |
| 2 | human | 2888 | GRB14 | growth factor receptor boun... | XM_024452815.1 | 86.2% | 86.2% | 0_1ins222;57G>T |
| 3 | human | 2888 | GRB14 | growth factor receptor boun... | XM_024452816.1 | 86.2% | 86.2% | 0_1ins222;57G>T |
| 4 | human | 2888 | GRB14 | growth factor receptor boun... | NM_001303422.2 | 81.3% | 78.6% | (many diffs) |
| 5 | human | 2888 | GRB14 | growth factor receptor boun... | XR_427085.3 | 66% | (many diffs) | |
| 6 | human | 2888 | GRB14 | growth factor receptor boun... | XM_017003899.1 | 62.9% | 62.9% | 0_1ins600 |
| 7 | human | 2888 | GRB14 | growth factor receptor boun... | XM_011511022.2 | 57.5% | 57.2% | (many diffs) |
| 8 | mouse | 50915 | Grb14 | growth factor receptor boun... | NM_016719.1 | 85% | 85.7% | (many diffs) |
| 9 | mouse | 50915 | Grb14 | growth factor receptor boun... | XM_006499883.3 | 71.2% | 69.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1686
- ORF length:
- 1620
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cacttccctg caagatgggc agagcgccgc gagcagggcg gctgcccggg 121 attcgccgct ggccgcccag gtgtgtggcg ctgcccaggg gaggggcgac gcccacgacc 181 tggcgccggc cccctggctg cacgcgcgag cgctcctgcc ccttccggac gggacccgcg 241 gctgtgctgc agacaggaga aaaaagaaag atcttgatgt tccggaaatg ccatctattc 301 caaacccttt tcctgagcta tgctgttctc catttacatc tgttttgtca gcagacctat 361 ttcccaaagc aaattcaagg aaaaaacagg tgattaaagt atacagtgaa gatgaaacca 421 gcagggcttt agatgtaccc agtgacataa cggctcgaga tgtttgtcag ctgttgatcc 481 tgaagaatca ttacattgat gaccacagct ggaccctttt tgagcacctg cctcacatag 541 gtgtagaaag aacaatagaa gaccacgaac tggtgattga agtgctatcc aactggggga 601 tagaagaaga aaacaaacta tactttagaa aaaattatgc caaatatgag ttctttaaaa 661 acccaatgta tttttttcca gagcatatgg tatcttttgc aactgaaacc aatggtgaaa 721 tatcccccac acagattttg cagatgtttc tgagttcaag cacatatcct gaaattcatg 781 gtttcttaca tgcgaaagaa cagggaaaga agtcttggaa aaaaatttac ttttttctaa 841 gaagatctgg tttatatttt tctactaaag gaacatcaaa ggaaccgcgg catttgcagt 901 ttttcagcga atttggcaat agtgatattt atgtgtcact ggcaggcaaa aaaaaacatg 961 gagcaccgac taactatgga ttctgcttta agcctaacaa agcgggaggg ccccgagacc 1021 tgaaaatgct ctgtgcagaa gaagagcaga gtaggacgtg ctgggtgacc gcgattagat 1081 tgcttaagta tggcatgcag ctgtaccaga attatatgca tccatatcaa ggtagaagtg 1141 gctgcagttc acagagcata tcacctatga gaagtatatc agagaattcc ctggtagcaa 1201 tggacttctc aggccagaaa agcagagtta tagaaaatcc cactgaagcc ctttcagttg 1261 cggttgaaga aggactcgct tGGAGGAAAA AAGGATGTTT ACGCCTGGGC ACTCACGGTA 1321 GCCCCACTGC CTCTTCACAG AGCTCTGCCA CAAACATGGC TATCCACCGG TCCCAGCCAT 1381 GGTTTCACCA CAAAATTTCT AGAGATGAGG CTCAGCGATT GATTATTCAG CAAGGACTTG 1441 TGGATGGAGT TTTCTTGGTA CGGGATAGTC AGAGTAACCC CAAAACTTTC GTACTGTCAA 1501 TGAGTCATGG ACAAAAAATA AAGCACTTTC AAATTATACC AGTAGAAGAT GACGGTGAAA 1561 TGTTCCACAC ACTGGATGAT GGCCACACAA GATTTACAGA TCTAATACAG CTGGTGGAGT 1621 TCTATCAACT CAATAAGGGC GTTCTTCCTT GCAAGTTGAA ACATTATTGT GCTAGGATTG 1681 CTCTCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1741 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1801 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGGTTAAGAT AAAGCCTCCC TTCAAACGCG 1861 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt