Construct: ORF TRCN0000478548

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006099.2_s317c1
Derived from:
ccsbBroadEn_06325
DNA Barcode:
GGTTAAGATAAAGCCTCCCTTCAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRB14 (2888)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478548
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2888 GRB14 growth factor receptor boun... NM_004490.3 99.9% 100% 279G>T
2 human 2888 GRB14 growth factor receptor boun... XM_024452815.1 86.2% 86.2% 0_1ins222;57G>T
3 human 2888 GRB14 growth factor receptor boun... XM_024452816.1 86.2% 86.2% 0_1ins222;57G>T
4 human 2888 GRB14 growth factor receptor boun... NM_001303422.2 81.3% 78.6% (many diffs)
5 human 2888 GRB14 growth factor receptor boun... XR_427085.3 66% (many diffs)
6 human 2888 GRB14 growth factor receptor boun... XM_017003899.1 62.9% 62.9% 0_1ins600
7 human 2888 GRB14 growth factor receptor boun... XM_011511022.2 57.5% 57.2% (many diffs)
8 mouse 50915 Grb14 growth factor receptor boun... NM_016719.1 85% 85.7% (many diffs)
9 mouse 50915 Grb14 growth factor receptor boun... XM_006499883.3 71.2% 69.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1686
ORF length:
1620
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac cacttccctg caagatgggc agagcgccgc gagcagggcg gctgcccggg
121attcgccgct ggccgcccag gtgtgtggcg ctgcccaggg gaggggcgac gcccacgacc
181tggcgccggc cccctggctg cacgcgcgag cgctcctgcc ccttccggac gggacccgcg
241gctgtgctgc agacaggaga aaaaagaaag atcttgatgt tccggaaatg ccatctattc
301caaacccttt tcctgagcta tgctgttctc catttacatc tgttttgtca gcagacctat
361ttcccaaagc aaattcaagg aaaaaacagg tgattaaagt atacagtgaa gatgaaacca
421gcagggcttt agatgtaccc agtgacataa cggctcgaga tgtttgtcag ctgttgatcc
481tgaagaatca ttacattgat gaccacagct ggaccctttt tgagcacctg cctcacatag
541gtgtagaaag aacaatagaa gaccacgaac tggtgattga agtgctatcc aactggggga
601tagaagaaga aaacaaacta tactttagaa aaaattatgc caaatatgag ttctttaaaa
661acccaatgta tttttttcca gagcatatgg tatcttttgc aactgaaacc aatggtgaaa
721tatcccccac acagattttg cagatgtttc tgagttcaag cacatatcct gaaattcatg
781gtttcttaca tgcgaaagaa cagggaaaga agtcttggaa aaaaatttac ttttttctaa
841gaagatctgg tttatatttt tctactaaag gaacatcaaa ggaaccgcgg catttgcagt
901ttttcagcga atttggcaat agtgatattt atgtgtcact ggcaggcaaa aaaaaacatg
961gagcaccgac taactatgga ttctgcttta agcctaacaa agcgggaggg ccccgagacc
1021tgaaaatgct ctgtgcagaa gaagagcaga gtaggacgtg ctgggtgacc gcgattagat
1081tgcttaagta tggcatgcag ctgtaccaga attatatgca tccatatcaa ggtagaagtg
1141gctgcagttc acagagcata tcacctatga gaagtatatc agagaattcc ctggtagcaa
1201tggacttctc aggccagaaa agcagagtta tagaaaatcc cactgaagcc ctttcagttg
1261cggttgaaga aggactcgct tGGAGGAAAA AAGGATGTTT ACGCCTGGGC ACTCACGGTA
1321GCCCCACTGC CTCTTCACAG AGCTCTGCCA CAAACATGGC TATCCACCGG TCCCAGCCAT
1381GGTTTCACCA CAAAATTTCT AGAGATGAGG CTCAGCGATT GATTATTCAG CAAGGACTTG
1441TGGATGGAGT TTTCTTGGTA CGGGATAGTC AGAGTAACCC CAAAACTTTC GTACTGTCAA
1501TGAGTCATGG ACAAAAAATA AAGCACTTTC AAATTATACC AGTAGAAGAT GACGGTGAAA
1561TGTTCCACAC ACTGGATGAT GGCCACACAA GATTTACAGA TCTAATACAG CTGGTGGAGT
1621TCTATCAACT CAATAAGGGC GTTCTTCCTT GCAAGTTGAA ACATTATTGT GCTAGGATTG
1681CTCTCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
1741TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
1801CTTGGCTTTA TATATCTTGT GGAAAGGACG AGGTTAAGAT AAAGCCTCCC TTCAAACGCG
1861TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)