Transcript: Human XR_938769.2

PREDICTED: Homo sapiens methyltransferase like 14 (METTL14), transcript variant X2, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
METTL14 (57721)
Length:
2729
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_938769.2
NBCI Gene record:
METTL14 (57721)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_938769.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000015935 GCCGTGTTAAATAGCAAAGAT pLKO.1 257 3UTR 100% 5.625 7.875 N METTL14 n/a
2 TRCN0000015937 GCTAATGTTGACATTGACTTA pLKO.1 1129 3UTR 100% 4.950 6.930 N METTL14 n/a
3 TRCN0000015933 CCATGTACTTACAAGCCGATA pLKO.1 660 3UTR 100% 4.050 5.670 N METTL14 n/a
4 TRCN0000431660 GAACCTGAAATTGGCAATATA pLKO_005 1162 3UTR 100% 15.000 10.500 N METTL14 n/a
5 TRCN0000424895 AGGATGAGTTAATAGCTAAAT pLKO_005 627 3UTR 100% 13.200 9.240 N METTL14 n/a
6 TRCN0000435430 GAAGACGCCTTCATCTATTTG pLKO_005 1232 3UTR 100% 13.200 9.240 N METTL14 n/a
7 TRCN0000015936 GCCGTGGACGAGAAAGAAATA pLKO.1 1481 3UTR 100% 13.200 9.240 N METTL14 n/a
8 TRCN0000015934 GCTTACAAATAGCAACTACAA pLKO.1 1299 3UTR 100% 4.950 3.465 N METTL14 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_938769.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03848 pDONR223 100% 50.1% None 1_160del;898_947del;1579_2729del n/a
2 ccsbBroad304_03848 pLX_304 0% 50.1% V5 1_160del;898_947del;1579_2729del n/a
3 TRCN0000468454 CATGACGGTATTAGCTCCCTGCCC pLX_317 34% 50.1% V5 1_160del;898_947del;1579_2729del n/a
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