Construct: ORF TRCN0000468454
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005676.1_s317c1
- Derived from:
- ccsbBroadEn_03848
- DNA Barcode:
- CATGACGGTATTAGCTCCCTGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- METTL14 (57721)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468454
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57721 | METTL14 | methyltransferase like 14 | NM_020961.4 | 100% | 100% | |
| 2 | human | 57721 | METTL14 | methyltransferase like 14 | XM_005263166.4 | 68.8% | 63.1% | 853_854ins211;942_943ins215 |
| 3 | human | 57721 | METTL14 | methyltransferase like 14 | XM_011532162.2 | 58% | 57.6% | 738_787del;873_874ins545 |
| 4 | human | 57721 | METTL14 | methyltransferase like 14 | XR_938769.2 | 50.1% | 1_160del;898_947del;1579_2729del | |
| 5 | mouse | 210529 | Mettl14 | methyltransferase like 14 | NM_201638.2 | 91.2% | 98.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1434
- ORF length:
- 1368
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tagccgcttg caggagatcc gggagcggca gaagttacgg cgacagctcc 121 tcgcgcagca gttgggagct gaaagtgccg acagcattgg tgccgtgtta aatagcaaag 181 atgagcagag agaaattgct gaaacaagag aaacttgcag ggcttcctat gatacctctg 241 ctccaaatgc aaaacgtaag tatctggatg aaggagagac agatgaagac aaaatggaag 301 aatataagga tgaactagaa atgcaacagg atgaagaaaa tttgccatat gaagaagaga 361 tttacaaaga ttctagtact tttcttaagg gaacacagag cttaaatccc cataatgatt 421 actgccaaca ttttgtagac actggacata gacctcagaa tttcatcagg gatgtaggtt 481 tagctgacag atttgaagaa tatcctaaac tgagggagct catcaggcta aaggatgagt 541 taatagctaa atctaacact cctcccatgt acttacaagc cgatatagaa gcctttgaca 601 tcagagaact aacacccaaa tttgatgtga ttcttctgga acccccttta gaagaatatt 661 acagagaaac tggcatcact gctaatgaaa aatgctggac ttgggatgat attatgaagt 721 tagaaattga tgagattgca gcacctcgat catttatttt tctctggtgt ggttctgggg 781 aggggttgga ccttggaaga gtgtgtttac gaaaatgggg ttacagaaga tgtgaagata 841 tttgttggat taaaaccaat aaaaacaatc ctgggaagac taagacttta gatccaaagg 901 ctgtctttca gagaacaaag gaacactgcc tcatggggat caaaggaact gtgaagcgta 961 gcacagacgg gGACTTCATT CATGCTAATG TTGACATTGA CTTAATTATC ACAGAAGAAC 1021 CTGAAATTGG CAATATAGAA AAACCTGTAG AAATTTTTCA TATAATTGAG CATTTTTGTC 1081 TTGGTAGAAG ACGCCTTCAT CTATTTGGAA GAGATAGTAC AATTCGACCA GGCTGGCTCA 1141 CAGTTGGACC AACGCTTACA AATAGCAACT ACAATGCAGA AACATATGCA TCCTATTTCA 1201 GTGCTCCTAA TTCCTACTTG ACTGGTTGTA CAGAAGAAAT TGAGAGACTT CGACCAAAAT 1261 CGCCTCCTCC CAAATCTAAA TCTGACCGAG GAGGTGGAGC TCCCAGAGGT GGAGGAAGAG 1321 GTGGAACTTC TGCTGGCCGT GGACGAGAAA GAAATAGATC TAACTTCCGA GGAGAAAGAG 1381 GTGGCTTTAG AGGGGGCCGT GGAGGAGCAC ACAGAGGTGG CTTTCCACCT CGATACCCAA 1441 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1501 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1561 TATCTTGTGG AAAGGACGAC ATGACGGTAT TAGCTCCCTG CCCACGCGTT AAGTCgacaa 1621 tcaacctctg gattacaaaa tttgtgaaag att