Transcript: Human XR_944580.2

PREDICTED: Homo sapiens 5'-nucleotidase domain containing 3 (NT5DC3), transcript variant X6, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NT5DC3 (51559)
Length:
1479
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_944580.2
NBCI Gene record:
NT5DC3 (51559)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_944580.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000167672 GCAGTATTAATGAAGATCGAT pLKO.1 544 3UTR 100% 3.000 4.200 N NT5DC3 n/a
2 TRCN0000167103 CCTGTCAGACATTGAAATCTA pLKO.1 351 3UTR 100% 5.625 3.938 N NT5DC3 n/a
3 TRCN0000168209 CTCTCATGGAAACACGATGAA pLKO.1 693 3UTR 100% 4.950 3.465 N NT5DC3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_944580.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03334 pDONR223 100% 69.1% None 1_75del;1264_1390del;1479_1480ins367 n/a
2 ccsbBroad304_03334 pLX_304 0% 69.1% V5 1_75del;1264_1390del;1479_1480ins367 n/a
3 TRCN0000477695 TGCACGACCCTAAAAAGAGGGTAT pLX_317 8.7% 69.1% V5 1_75del;1264_1390del;1479_1480ins367 n/a
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