Construct: ORF TRCN0000477695
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001235.1_s317c1
- Derived from:
- ccsbBroadEn_03334
- DNA Barcode:
- TGCACGACCCTAAAAAGAGGGTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NT5DC3 (51559)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477695
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51559 | NT5DC3 | 5'-nucleotidase domain cont... | NM_001031701.3 | 100% | 100% | |
| 2 | human | 51559 | NT5DC3 | 5'-nucleotidase domain cont... | XR_001748759.1 | 72.2% | 1_75del;1264_1440del;1622_1623ins274 | |
| 3 | human | 51559 | NT5DC3 | 5'-nucleotidase domain cont... | XM_011538476.2 | 71.7% | 68.9% | (many diffs) |
| 4 | human | 51559 | NT5DC3 | 5'-nucleotidase domain cont... | XR_944581.2 | 69.4% | 1_77del;1094_1095ins82;1272_1273ins367 | |
| 5 | human | 51559 | NT5DC3 | 5'-nucleotidase domain cont... | XR_944580.2 | 69.1% | 1_75del;1264_1390del;1479_1480ins367 | |
| 6 | human | 51559 | NT5DC3 | 5'-nucleotidase domain cont... | XR_001748760.2 | 68.7% | (many diffs) | |
| 7 | human | 51559 | NT5DC3 | 5'-nucleotidase domain cont... | XR_944582.2 | 67.2% | (many diffs) | |
| 8 | human | 51559 | NT5DC3 | 5'-nucleotidase domain cont... | XM_017019454.1 | 66.1% | 60.9% | (many diffs) |
| 9 | human | 51559 | NT5DC3 | 5'-nucleotidase domain cont... | XR_001748757.1 | 46% | (many diffs) | |
| 10 | human | 51559 | NT5DC3 | 5'-nucleotidase domain cont... | XR_001748756.1 | 35.1% | 1_56del;1701_4674del | |
| 11 | human | 51559 | NT5DC3 | 5'-nucleotidase domain cont... | XR_001748758.1 | 34.4% | (many diffs) | |
| 12 | human | 51559 | NT5DC3 | 5'-nucleotidase domain cont... | XR_944574.2 | 33.8% | 1_56del;1701_4856del | |
| 13 | mouse | 103466 | Nt5dc3 | 5'-nucleotidase domain cont... | NM_175331.3 | 85.6% | 91.2% | (many diffs) |
| 14 | mouse | 103466 | Nt5dc3 | 5'-nucleotidase domain cont... | XM_006513017.3 | 49.9% | 48.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1710
- ORF length:
- 1644
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac catggcagcg gcggcggtgg tggcacgcgg ggccggggcg agggcagcga 121 cagcggcggc tttgcggggt ggctgcggga ccgcggctcg ggggcggccg tgtgcgggcc 181 ccgcccggcc cttgtgcact gcacccggga ccgccccgga catgaagcgc tacctgtggg 241 agcgctaccg ggaggcgaag agaagcacag aagaattggt tccttccatt atgagcaact 301 tgttgaatcc agatgccatt ttctcaaaca atgaaatgag cctgtcagac attgaaatct 361 atggcttcga ttatgattac accttggtgt tttattcaaa gcacctccac acgctgatat 421 ttaatgctgc acgggacctt ctcatcaatg aacaccggta tccagcagaa atcaggaagt 481 atgagtatga cccaaatttt gcaattcgtg gacttcatta tgatgtacag cgggcagtat 541 taatgaagat cgatgctttt cattatatcc agctgggaac tgtctacaga ggcctcagtg 601 ttgtccctga tgaagaagtc attgaaatgt acgaggggtc ccacgtgccc ttggagcaga 661 tgagtgactt ttacggaaag agctctcatg gaaacacgat gaagcagttc atggacatct 721 tctccctgcc cgagatgacc ctcctgtcct gcgtgaatga atacttcctc aagaacaaca 781 tcgactatga gcctgtgcat ctgtacaaag atgtcaagga ttcaattcga gacgtccaca 841 tcaaaggaat aatgtacaga gcaattgaag cagacattga aaagtacatc tgctatgctg 901 agcagacccg cgcagtgttg gccaaactgg ctgatcatgg caagaagatg tttctcatca 961 ccaatagccc cagtagcttt gtggacaaag ggatgagtta tatcgttggg aaagactgga 1021 gggacctgtt cgatgtggtc attgttcagg ctgagaagcc aaacttcttt aatgataagc 1081 ggaggccttt ccgaaagatg aatgagaaag gtgtcttact ctgggataaa atccataagt 1141 tgcagaaagg ccagatatac aagcagggta atttatatga atttttgaag cttactggat 1201 ggagaggatc cagagtgttg tattttggtg accatatata cagtgacctg gcggatttga 1261 ccctaaagca tggctggagg actggtgcaa tcatcccaga gttgagatct gagctcaaaa 1321 tcatgaacac ggagcaatac attcaaacca tgacctggct gcagaccttg actggcttat 1381 tggaacagat gcaggttcac agagatgctg agtcacagct ggttttgcag gagtggaaaa 1441 aggaaaggaa ggagatgcga gaaatgacca agagtttctt caatgcccag tttggaagcc 1501 tgttccgcac agaccagaac ccaacctact tcctaaggcg cctgtcgcgc ttcgctgaca 1561 tctacatggc gtctcTGAGC TGCCTCCTGA ACTATGACGT CAGCCACACT TTCTACCCCC 1621 GGAGGACTCC ACTGCAGCAC GAACTGCCCG CCTGGTCAGA AAGGCCCCCC ACCTTCGGAA 1681 CCCCTCTCCT GCAGGAGGCC CAGGCCAAGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1741 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1801 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATGCAC 1861 GACCCTAAAA AGAGGGTATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1921 tgaaagatt