Transcript: Human XR_946699.2

PREDICTED: Homo sapiens mucolipin 3 (MCOLN3), transcript variant X5, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MCOLN3 (55283)
Length:
6164
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_946699.2
NBCI Gene record:
MCOLN3 (55283)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_946699.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000416641 TTGACAATCATTGGATCAATT pLKO_005 1129 3UTR 100% 13.200 10.560 N MCOLN3 n/a
2 TRCN0000420375 ACTATCCAGCTGGTCTTATTT pLKO_005 298 3UTR 100% 15.000 10.500 N MCOLN3 n/a
3 TRCN0000428091 GATGACCCTCCAGTATCTTTA pLKO_005 5082 3UTR 100% 13.200 9.240 N MCOLN3 n/a
4 TRCN0000074312 TCAGTTAATCTTCGCAGTAAA pLKO.1 450 3UTR 100% 13.200 9.240 N MCOLN3 n/a
5 TRCN0000074311 CCATGGAAACTTGCCATACAA pLKO.1 256 3UTR 100% 5.625 3.938 N MCOLN3 n/a
6 TRCN0000074309 CTGGCATGTATCTGGATCAAT pLKO.1 891 3UTR 100% 5.625 3.938 N MCOLN3 n/a
7 TRCN0000074310 GAACACTCATTACATGATGAT pLKO.1 918 3UTR 100% 4.950 3.465 N MCOLN3 n/a
8 TRCN0000074308 GCACCAGCTAACAATGTGATA pLKO.1 5653 3UTR 100% 4.950 3.465 N MCOLN3 n/a
9 TRCN0000430431 GAGTGGGTGTAGTTATCATTT pLKO_005 5548 3UTR 100% 13.200 7.920 N MCOLN3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_946699.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12197 pDONR223 100% 15.5% None (many diffs) n/a
2 ccsbBroad304_12197 pLX_304 0% 15.5% V5 (many diffs) n/a
3 TRCN0000478835 GCCATGTGATAGAGAATTGTTCGG pLX_317 45.7% 15.5% V5 (many diffs) n/a
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