Construct: sgRNA BRDN0001147780
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAGGCTGAAGTTCGTACCT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80226
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
98155675 |
+ |
LINC02295 |
NNNGGCTGAAATTCATACCT |
NGG |
2 |
0.9412 |
Tier III |
| 2 |
NC_000014.9 |
14 |
98155675 |
+ |
LOC105370655 |
NNNGGCTGAAATTCATACCT |
NGG |
2 |
0.9412 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
28142309 |
- |
9530053A07Rik |
NNNGGCTGAAGTTCGTGCGT |
NGG |
2 |
0.0221 |
Tier I |
| 2 |
NC_000072.6 |
6 |
17464142 |
- |
Met |
NNNGGCTGGAGTTTGTACCT |
NGG |
2 |
0.4889 |
Tier II |
| 3 |
NC_000076.6 |
10 |
76009310 |
- |
Gm5134 |
NNNGGCTGAAGTTCGCACCA |
NGG |
2 |
0.375 |
Tier II |
| 4 |
NC_000069.6 |
3 |
127200848 |
- |
Ank2 |
NNNTACTGAAGTTCGTACCT |
NGG |
2 |
0.3152 |
Tier II |
| 5 |
NC_000084.6 |
18 |
39363265 |
+ |
Arhgap26 |
NNNGGCTGAAGTTCCTACCT |
NGG |
1 |
0.2727 |
Tier II |
| 6 |
NC_000071.6 |
5 |
108684985 |
+ |
Idua |
NNNGGCTGATGTTCCTACCT |
NGG |
2 |
0.2406 |
Tier II |
| 7 |
NC_000079.6 |
13 |
24137580 |
- |
Carmil1 |
NNNTGCTGCAGTTCGTACCT |
NGG |
2 |
0.2078 |
Tier II |
| 8 |
NC_000077.6 |
11 |
104264935 |
+ |
Mapt |
NNNGGCTGAAGTTCATGCCT |
NGG |
2 |
0.1661 |
Tier II |
| 9 |
NC_000074.6 |
8 |
95113270 |
+ |
Kifc3 |
NNNGGCTGAAGTTCTGACCT |
NGG |
2 |
0.0495 |
Tier II |
| 10 |
NC_000084.6 |
18 |
39363265 |
+ |
Gm15337 |
NNNGGCTGAAGTTCCTACCT |
NGG |
1 |
0.2727 |
Tier III |
| 11 |
NC_000071.6 |
5 |
108684985 |
+ |
Gm42151 |
NNNGGCTGATGTTCCTACCT |
NGG |
2 |
0.2406 |
Tier III |
| 12 |
NC_000076.6 |
10 |
21181469 |
+ |
Gm40608 |
NNNGGCTGAAGTTCTTGCCT |
NGG |
2 |
0.0252 |
Tier III |
| 13 |
NC_000073.6 |
7 |
28142309 |
- |
Gm21925 |
NNNGGCTGAAGTTCGTGCGT |
NGG |
2 |
0.0221 |
Tier III |
Other clones with same target sequence:
(none)
