Construct: sgRNA BRDN0001148219
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATCGATATACCGCCATAAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80195
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
29374557 |
+ |
OR12D3 |
NNNGACATACCACCATAAAA |
NGG |
2 |
0.8485 |
Tier I |
| 2 |
NC_000004.12 |
4 |
163658613 |
+ |
MARCHF1 |
NNNAATATACAGCCATAAAA |
NGG |
2 |
0.675 |
Tier II |
| 3 |
NC_000009.12 |
9 |
5420043 |
+ |
PLGRKT |
NNNGATATTCAGCCATAAAA |
NGG |
2 |
0.45 |
Tier II |
| 4 |
NC_000011.10 |
11 |
26482937 |
- |
ANO3 |
NNNGATATTCAGCCATAAAA |
NGG |
2 |
0.45 |
Tier II |
| 5 |
NC_000007.14 |
7 |
141285766 |
+ |
TMEM178B |
NNNGCTATACAGCCATAAAA |
NGG |
2 |
0.375 |
Tier II |
| 6 |
NC_000002.12 |
2 |
115627869 |
- |
DPP10 |
NNNGTTATACAGCCATAAAA |
NGG |
2 |
0.2727 |
Tier II |
| 7 |
NC_000013.11 |
13 |
45532195 |
- |
COG3 |
NNNTATATACAGCCATAAAA |
NGG |
2 |
0.2727 |
Tier II |
| 8 |
NC_000012.12 |
12 |
14440942 |
- |
ATF7IP |
NNNCATATACCGCCATATAA |
NGG |
2 |
0.2647 |
Tier II |
| 9 |
NC_000013.11 |
13 |
26344237 |
- |
CDK8 |
NNNGATATACAGCCATAAAA |
NAG |
2 |
0.1944 |
Tier II |
| 10 |
NC_000016.10 |
16 |
6606698 |
- |
RBFOX1 |
NNNGCTATACGGCCATAAAA |
NGG |
2 |
0.125 |
Tier II |
| 11 |
NC_000006.12 |
6 |
149011121 |
+ |
UST |
NNNGATATGCCGCCATAAAA |
NTG |
2 |
0.026 |
Tier II |
| 12 |
NC_000016.10 |
16 |
65497030 |
+ |
LINC00922 |
NNNGATATACAGCCATAAAA |
NGG |
1 |
0.75 |
Tier III |
| 13 |
NC_000008.11 |
8 |
5979526 |
- |
LOC105377795 |
NNNAATATGCCGCCATAAAA |
NGG |
2 |
0.6 |
Tier III |
| 14 |
NC_000006.12 |
6 |
113108309 |
- |
LOC105377952 |
NNNGCTATACAGCCATAAAA |
NGG |
2 |
0.375 |
Tier III |
| 15 |
NC_000005.10 |
5 |
3469882 |
- |
LINC01019 |
NNNGTTATAGCGCCATAAAA |
NGG |
2 |
0.1414 |
Tier III |
| 16 |
NC_000007.14 |
7 |
34599727 |
- |
NPSR1-AS1 |
NNNGATATACTGCCATCAAA |
NGG |
2 |
0.0543 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
104523662 |
- |
Thoc2l |
NNNAATATACAGCCATAAAA |
NGG |
2 |
0.675 |
Tier II |
| 2 |
NC_000072.6 |
6 |
131350229 |
- |
Styk1 |
NNNGAGATACAGCCATAAAA |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000075.6 |
9 |
84017543 |
- |
Bckdhb |
NNNGATATACTGCCAAAAAA |
NGG |
2 |
0.2797 |
Tier II |
| 4 |
NC_000082.6 |
16 |
91033693 |
+ |
Paxbp1 |
NNNGAAATACCGCCATAAAA |
NGT |
2 |
0.014 |
Tier II |
| 5 |
NC_000075.6 |
9 |
84017543 |
- |
Gm46124 |
NNNGATATACTGCCAAAAAA |
NGG |
2 |
0.2797 |
Tier III |
Other clones with same target sequence:
(none)
