Construct: sgRNA BRDN0001148219

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATCGATATACCGCCATAAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80195

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 29374557 + OR12D3 NNNGACATACCACCATAAAA NGG 2 0.8485 Tier I
2 NC_000004.12 4 163658613 + MARCHF1 NNNAATATACAGCCATAAAA NGG 2 0.675 Tier II
3 NC_000009.12 9 5420043 + PLGRKT NNNGATATTCAGCCATAAAA NGG 2 0.45 Tier II
4 NC_000011.10 11 26482937 - ANO3 NNNGATATTCAGCCATAAAA NGG 2 0.45 Tier II
5 NC_000007.14 7 141285766 + TMEM178B NNNGCTATACAGCCATAAAA NGG 2 0.375 Tier II
6 NC_000002.12 2 115627869 - DPP10 NNNGTTATACAGCCATAAAA NGG 2 0.2727 Tier II
7 NC_000013.11 13 45532195 - COG3 NNNTATATACAGCCATAAAA NGG 2 0.2727 Tier II
8 NC_000012.12 12 14440942 - ATF7IP NNNCATATACCGCCATATAA NGG 2 0.2647 Tier II
9 NC_000013.11 13 26344237 - CDK8 NNNGATATACAGCCATAAAA NAG 2 0.1944 Tier II
10 NC_000016.10 16 6606698 - RBFOX1 NNNGCTATACGGCCATAAAA NGG 2 0.125 Tier II
11 NC_000006.12 6 149011121 + UST NNNGATATGCCGCCATAAAA NTG 2 0.026 Tier II
12 NC_000016.10 16 65497030 + LINC00922 NNNGATATACAGCCATAAAA NGG 1 0.75 Tier III
13 NC_000008.11 8 5979526 - LOC105377795 NNNAATATGCCGCCATAAAA NGG 2 0.6 Tier III
14 NC_000006.12 6 113108309 - LOC105377952 NNNGCTATACAGCCATAAAA NGG 2 0.375 Tier III
15 NC_000005.10 5 3469882 - LINC01019 NNNGTTATAGCGCCATAAAA NGG 2 0.1414 Tier III
16 NC_000007.14 7 34599727 - NPSR1-AS1 NNNGATATACTGCCATCAAA NGG 2 0.0543 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 104523662 - Thoc2l NNNAATATACAGCCATAAAA NGG 2 0.675 Tier II
2 NC_000072.6 6 131350229 - Styk1 NNNGAGATACAGCCATAAAA NGG 2 0.4286 Tier II
3 NC_000075.6 9 84017543 - Bckdhb NNNGATATACTGCCAAAAAA NGG 2 0.2797 Tier II
4 NC_000082.6 16 91033693 + Paxbp1 NNNGAAATACCGCCATAAAA NGT 2 0.014 Tier II
5 NC_000075.6 9 84017543 - Gm46124 NNNGATATACTGCCAAAAAA NGG 2 0.2797 Tier III
Download CSV

Other clones with same target sequence:

(none)

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