Construct: ORF TRCN0000465270

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000426.1_s317c1
Derived from:
ccsbBroadEn_06326
DNA Barcode:
CATAATTAAGGTCCATATACTGTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRIA1 (2890)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465270
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2890 GRIA1 glutamate ionotropic recept... NM_000827.4 99.9% 100% 531T>C
2 human 2890 GRIA1 glutamate ionotropic recept... NM_001114183.1 98.5% 99.1% (many diffs)
3 human 2890 GRIA1 glutamate ionotropic recept... NM_001258021.1 97.4% 95.9% (many diffs)
4 human 2890 GRIA1 glutamate ionotropic recept... NM_001364166.1 96.1% 95.2% (many diffs)
5 human 2890 GRIA1 glutamate ionotropic recept... NM_001258022.1 96% 95.1% (many diffs)
6 human 2890 GRIA1 glutamate ionotropic recept... NM_001364165.1 93.7% 93.8% 531T>C;859_860ins168
7 human 2890 GRIA1 glutamate ionotropic recept... NM_001258023.1 92.3% 92.3% 0_1ins207;324T>C
8 human 2890 GRIA1 glutamate ionotropic recept... NM_001258019.1 91.1% 91.1% 220_221ins240;291T>C
9 human 2890 GRIA1 glutamate ionotropic recept... NM_001364167.1 90.9% 91.5% (many diffs)
10 human 2890 GRIA1 glutamate ionotropic recept... XM_017009392.1 89.1% 81.5% (many diffs)
11 human 2890 GRIA1 glutamate ionotropic recept... NM_001258020.1 88% 88.6% (many diffs)
12 human 2890 GRIA1 glutamate ionotropic recept... NR_157093.1 47.6% (many diffs)
13 human 2890 GRIA1 glutamate ionotropic recept... NR_047578.1 42.8% (many diffs)
14 mouse 14799 Gria1 glutamate receptor, ionotro... NM_008165.4 90.2% 98.6% (many diffs)
15 mouse 14799 Gria1 glutamate receptor, ionotro... NM_001113325.2 88.8% 97.7% (many diffs)
16 mouse 14799 Gria1 glutamate receptor, ionotro... XM_017314275.1 83.2% 91.2% (many diffs)
17 mouse 14799 Gria1 glutamate receptor, ionotro... NM_001252403.1 81.9% 90.4% (many diffs)
18 mouse 14799 Gria1 glutamate receptor, ionotro... XM_006532234.3 81.9% 90.4% (many diffs)
19 mouse 14799 Gria1 glutamate receptor, ionotro... XR_388348.2 41.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2787
ORF length:
2718
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggccccat gcagcacatt tttgccttct tctgcaccgg tttcctaggc gcggtagtag
121gtgccaattt ccccaacaat atccagatcg ggggattatt tccaaaccag cagtcacagg
181aacatgctgc ttttagattt gctttgtcgc aactcacaga gcccccgaag ctgctccccc
241agattgatat tgtgaacatc agcgacagct ttgagatgac ctatagattc tgttcccagt
301tctccaaagg agtctatgcc atctttgggt tttatgaacg taggactgtc aacatgctga
361cctccttttg tggggccctc cacgtctgct tcattacgcc gagctttccc gttgatacat
421ccaatcagtt tgtccttcag ctgcgccctg aactgcagga tgccctcatc agcatcattg
481accattacaa gtggcagaaa tttgtctaca tttatgatgc cgaccggggc ttatccgtcc
541tgcagaaagt cctggataca gctgctgaga agaactggca ggtgacagca gtcaacatct
601tgacaaccac agaggaggga taccggatgc tctttcagga cctggagaag aaaaaggagc
661ggctggtggt ggtggactgt gaatcagaac gcctcaatgc tatcttgggc cagattataa
721agctagagaa gaatggcatc ggctaccact acattcttgc aaatctgggc ttcatggaca
781ttgacttaaa caaattcaag gagagtggcg ccaatgtgac aggtttccag ctggtgaact
841acacagacac tattccggcc aagatcatgc agcagtggaa gaatagtgat gctcgagacc
901acacacgggt ggactggaag agacccaagt acacctctgc gctcacctac gatggggtga
961aggtgatggc tgaggctttc cagagcctgc ggaggcagag aattgatata tctcgccggg
1021ggaatgctgg ggattgtctg gctaacccag ctgttccctg gggccaaggg atcgacatcc
1081agagagctct gcagcaggtg cgatttgaag gtttaacagg aaacgtgcag tttaatgaga
1141aaggacgccg gaccaactac acgctccacg tgattgaaat gaaacatgac ggcatccgaa
1201agattggtta ctggaatgaa gatgataagt ttgtccctgc agccaccgat gcccaagctg
1261ggggcgataa ttcaagtgtt cagaacagaa catacatcgt cacaacaatc ctagaagatc
1321cttatgtgat gctcaagaag aacgccaatc agtttgaggg caatgaccgt tacgagggct
1381actgtgtaga gctggcggca gagattgcca agcacgtggg ctactcctac cgtctggaga
1441ttgtcagtga tggaaaatac ggagcccgag accctgacac gaaggcctgg aatggcatgg
1501tgggagagct ggtctatgga agagcagatg tggctgtggc tcccttaact atcactttgg
1561tccgggaaga agttatagat ttctccaaac catttatgag tttggggatc tccatcatga
1621ttaaaaaacc acagaaatcc aagccgggtg tcttctcctt ccttgatcct ttggcttatg
1681agatttggat gtgcattgtt tttgcctaca ttggagtgag tgttgtcctc ttcctggtca
1741gccgcttcag tccctatgaa tggcacagtg aagagtttga ggaaggacgg gaccagacaa
1801ccagtgacca gtccaatgag tttgggatat tcaacagttt gtggttctcc ctgggagcct
1861tcatgcagca aggatgtgac atttctccca ggtccctgtc tggtcgcatc gttggtggcg
1921tctggtggtt cttcacctta atcatcatct cctcatatac agccaatctg gccgccttcc
1981tgaccgtgga gaggatggtg tctcccattg agagtgcaga ggacctagcg aagcagacag
2041aaattgccta cgggacgctg gaagcaggat ctactaagga gttcttcagg aggtctaaaa
2101ttgctgtgtt tgagaagatg tggacataca tgaagtcagc agagccatca gtttttgtgc
2161ggaccacaga ggaggggatg attcgagtga ggaaatccaa aggcaaatat gcctacctcc
2221tggagtccac catgaatgag tacattgagc agcggaaacc ctgtgacacc atgaaggtgg
2281gaggtaactt ggattccaaa ggctatggca ttgcaacacc caaggggtct gccctgagaa
2341atccagtaaa cctggcagtg ttaaaactga acgagcaggg gcttttggac aaattgaaaa
2401acaaatggtg gtacgacaag ggcgagtgcg gcagcggggg aggtgattcc aaggacaaga
2461caagcgctct gagccTCAGC AATGTGGCAG GCGTGTTCTA CATCCTGATC GGAGGACTTG
2521GACTAGCCAT GCTGGTTGCC TTAATCGAGT TCTGCTACAA ATCCCGTAGT GAATCCAAGC
2581GGATGAAGGG TTTTTGTTTG ATCCCACAGC AATCCATCAA CGAAGCCATA CGGACATCGA
2641CCCTCCCCCG CAACAGCGGG GCAGGAGCCA GCAGCGGCGG CAGTGGAGAG AATGGTCGGG
2701TGGTCAGCCA TGACTTCCCC AAGTCCATGC AATCGATTCC TTGCATGAGC CACAGTTCAG
2761GGATGCCCTT GGGAGCCACG GGATTGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG
2821GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
2881AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACATAATTA
2941AGGTCCATAT ACTGTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
3001aagatt

Download FASTA (ORF) (Full)