Construct: ORF TRCN0000465270
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000426.1_s317c1
- Derived from:
- ccsbBroadEn_06326
- DNA Barcode:
- CATAATTAAGGTCCATATACTGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRIA1 (2890)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465270
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2890 | GRIA1 | glutamate ionotropic recept... | NM_000827.4 | 99.9% | 100% | 531T>C |
| 2 | human | 2890 | GRIA1 | glutamate ionotropic recept... | NM_001114183.1 | 98.5% | 99.1% | (many diffs) |
| 3 | human | 2890 | GRIA1 | glutamate ionotropic recept... | NM_001258021.1 | 97.4% | 95.9% | (many diffs) |
| 4 | human | 2890 | GRIA1 | glutamate ionotropic recept... | NM_001364166.1 | 96.1% | 95.2% | (many diffs) |
| 5 | human | 2890 | GRIA1 | glutamate ionotropic recept... | NM_001258022.1 | 96% | 95.1% | (many diffs) |
| 6 | human | 2890 | GRIA1 | glutamate ionotropic recept... | NM_001364165.1 | 93.7% | 93.8% | 531T>C;859_860ins168 |
| 7 | human | 2890 | GRIA1 | glutamate ionotropic recept... | NM_001258023.1 | 92.3% | 92.3% | 0_1ins207;324T>C |
| 8 | human | 2890 | GRIA1 | glutamate ionotropic recept... | NM_001258019.1 | 91.1% | 91.1% | 220_221ins240;291T>C |
| 9 | human | 2890 | GRIA1 | glutamate ionotropic recept... | NM_001364167.1 | 90.9% | 91.5% | (many diffs) |
| 10 | human | 2890 | GRIA1 | glutamate ionotropic recept... | XM_017009392.1 | 89.1% | 81.5% | (many diffs) |
| 11 | human | 2890 | GRIA1 | glutamate ionotropic recept... | NM_001258020.1 | 88% | 88.6% | (many diffs) |
| 12 | human | 2890 | GRIA1 | glutamate ionotropic recept... | NR_157093.1 | 47.6% | (many diffs) | |
| 13 | human | 2890 | GRIA1 | glutamate ionotropic recept... | NR_047578.1 | 42.8% | (many diffs) | |
| 14 | mouse | 14799 | Gria1 | glutamate receptor, ionotro... | NM_008165.4 | 90.2% | 98.6% | (many diffs) |
| 15 | mouse | 14799 | Gria1 | glutamate receptor, ionotro... | NM_001113325.2 | 88.8% | 97.7% | (many diffs) |
| 16 | mouse | 14799 | Gria1 | glutamate receptor, ionotro... | XM_017314275.1 | 83.2% | 91.2% | (many diffs) |
| 17 | mouse | 14799 | Gria1 | glutamate receptor, ionotro... | NM_001252403.1 | 81.9% | 90.4% | (many diffs) |
| 18 | mouse | 14799 | Gria1 | glutamate receptor, ionotro... | XM_006532234.3 | 81.9% | 90.4% | (many diffs) |
| 19 | mouse | 14799 | Gria1 | glutamate receptor, ionotro... | XR_388348.2 | 41.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2787
- ORF length:
- 2718
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggccccat gcagcacatt tttgccttct tctgcaccgg tttcctaggc gcggtagtag 121 gtgccaattt ccccaacaat atccagatcg ggggattatt tccaaaccag cagtcacagg 181 aacatgctgc ttttagattt gctttgtcgc aactcacaga gcccccgaag ctgctccccc 241 agattgatat tgtgaacatc agcgacagct ttgagatgac ctatagattc tgttcccagt 301 tctccaaagg agtctatgcc atctttgggt tttatgaacg taggactgtc aacatgctga 361 cctccttttg tggggccctc cacgtctgct tcattacgcc gagctttccc gttgatacat 421 ccaatcagtt tgtccttcag ctgcgccctg aactgcagga tgccctcatc agcatcattg 481 accattacaa gtggcagaaa tttgtctaca tttatgatgc cgaccggggc ttatccgtcc 541 tgcagaaagt cctggataca gctgctgaga agaactggca ggtgacagca gtcaacatct 601 tgacaaccac agaggaggga taccggatgc tctttcagga cctggagaag aaaaaggagc 661 ggctggtggt ggtggactgt gaatcagaac gcctcaatgc tatcttgggc cagattataa 721 agctagagaa gaatggcatc ggctaccact acattcttgc aaatctgggc ttcatggaca 781 ttgacttaaa caaattcaag gagagtggcg ccaatgtgac aggtttccag ctggtgaact 841 acacagacac tattccggcc aagatcatgc agcagtggaa gaatagtgat gctcgagacc 901 acacacgggt ggactggaag agacccaagt acacctctgc gctcacctac gatggggtga 961 aggtgatggc tgaggctttc cagagcctgc ggaggcagag aattgatata tctcgccggg 1021 ggaatgctgg ggattgtctg gctaacccag ctgttccctg gggccaaggg atcgacatcc 1081 agagagctct gcagcaggtg cgatttgaag gtttaacagg aaacgtgcag tttaatgaga 1141 aaggacgccg gaccaactac acgctccacg tgattgaaat gaaacatgac ggcatccgaa 1201 agattggtta ctggaatgaa gatgataagt ttgtccctgc agccaccgat gcccaagctg 1261 ggggcgataa ttcaagtgtt cagaacagaa catacatcgt cacaacaatc ctagaagatc 1321 cttatgtgat gctcaagaag aacgccaatc agtttgaggg caatgaccgt tacgagggct 1381 actgtgtaga gctggcggca gagattgcca agcacgtggg ctactcctac cgtctggaga 1441 ttgtcagtga tggaaaatac ggagcccgag accctgacac gaaggcctgg aatggcatgg 1501 tgggagagct ggtctatgga agagcagatg tggctgtggc tcccttaact atcactttgg 1561 tccgggaaga agttatagat ttctccaaac catttatgag tttggggatc tccatcatga 1621 ttaaaaaacc acagaaatcc aagccgggtg tcttctcctt ccttgatcct ttggcttatg 1681 agatttggat gtgcattgtt tttgcctaca ttggagtgag tgttgtcctc ttcctggtca 1741 gccgcttcag tccctatgaa tggcacagtg aagagtttga ggaaggacgg gaccagacaa 1801 ccagtgacca gtccaatgag tttgggatat tcaacagttt gtggttctcc ctgggagcct 1861 tcatgcagca aggatgtgac atttctccca ggtccctgtc tggtcgcatc gttggtggcg 1921 tctggtggtt cttcacctta atcatcatct cctcatatac agccaatctg gccgccttcc 1981 tgaccgtgga gaggatggtg tctcccattg agagtgcaga ggacctagcg aagcagacag 2041 aaattgccta cgggacgctg gaagcaggat ctactaagga gttcttcagg aggtctaaaa 2101 ttgctgtgtt tgagaagatg tggacataca tgaagtcagc agagccatca gtttttgtgc 2161 ggaccacaga ggaggggatg attcgagtga ggaaatccaa aggcaaatat gcctacctcc 2221 tggagtccac catgaatgag tacattgagc agcggaaacc ctgtgacacc atgaaggtgg 2281 gaggtaactt ggattccaaa ggctatggca ttgcaacacc caaggggtct gccctgagaa 2341 atccagtaaa cctggcagtg ttaaaactga acgagcaggg gcttttggac aaattgaaaa 2401 acaaatggtg gtacgacaag ggcgagtgcg gcagcggggg aggtgattcc aaggacaaga 2461 caagcgctct gagccTCAGC AATGTGGCAG GCGTGTTCTA CATCCTGATC GGAGGACTTG 2521 GACTAGCCAT GCTGGTTGCC TTAATCGAGT TCTGCTACAA ATCCCGTAGT GAATCCAAGC 2581 GGATGAAGGG TTTTTGTTTG ATCCCACAGC AATCCATCAA CGAAGCCATA CGGACATCGA 2641 CCCTCCCCCG CAACAGCGGG GCAGGAGCCA GCAGCGGCGG CAGTGGAGAG AATGGTCGGG 2701 TGGTCAGCCA TGACTTCCCC AAGTCCATGC AATCGATTCC TTGCATGAGC CACAGTTCAG 2761 GGATGCCCTT GGGAGCCACG GGATTGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 2821 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2881 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACATAATTA 2941 AGGTCCATAT ACTGTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3001 aagatt