Construct: ORF TRCN0000465353
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012372.1_s317c1
- Derived from:
- ccsbBroadEn_05861
- DNA Barcode:
- ACTAATAACATTGGTCCGTTAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASS1 (445)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465353
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 445 | ASS1 | argininosuccinate synthase 1 | NM_000050.4 | 99.9% | 100% | 876T>C |
| 2 | human | 445 | ASS1 | argininosuccinate synthase 1 | NM_054012.4 | 99.9% | 100% | 876T>C |
| 3 | human | 445 | ASS1 | argininosuccinate synthase 1 | XM_005272200.3 | 99.9% | 100% | 876T>C |
| 4 | human | 445 | ASS1 | argininosuccinate synthase 1 | XM_017014729.1 | 92.7% | 92.7% | 1_96del;972T>C |
| 5 | human | 445 | ASS1 | argininosuccinate synthase 1 | XM_011518705.2 | 91.4% | 91.5% | 1_114del;990T>C |
| 6 | mouse | 11898 | Ass1 | argininosuccinate synthetase 1 | NM_007494.3 | 89.1% | 96.8% | (many diffs) |
| 7 | mouse | 432466 | Gm5424 | predicted gene 5424; argini... | NR_002687.1 | 71.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1302
- ORF length:
- 1236
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cagcaaaggc tccgtggttc tggcctacag tggcggcctg gacacctcgt 121 gcatcctcgt gtggctgaag gaacaaggct atgacgtcat tgcctatctg gccaacattg 181 gccagaagga agacttcgag gaagccagga agaaggcact gaagcttggg gccaaaaagg 241 tgttcattga ggatgtcagc agggagtttg tggaggagtt catctggccg gccatccagt 301 ccagcgcact gtatgaggac cgctacctcc tgggcacctc tcttgccagg ccctgcatcg 361 cccgcaaaca agtggaaatc gcccagcggg agggggccaa gtatgtgtcc cacggcgcca 421 caggaaaggg gaacgatcag gtccggtttg agctcagctg ctactcactg gccccccaga 481 taaaggtcat tgctccctgg aggatgcctg aattctacaa ccggttcaag ggccgcaatg 541 acctgatgga gtacgcaaag caacacggga ttcccatccc ggtcactccc aagaacccgt 601 ggagcatgga tgagaacctc atgcacatca gctacgaggc tggaatcctg gagaacccca 661 agaaccaagc gcctccaggt ctctacacga agacccagga cccagccaaa gcccccaaca 721 cccctgacat tctcgagatc gagttcaaaa aaggggtccc tgtgaaggtg accaacgtca 781 aggatggcac cacccaccag acctccttgg agctcttcat gtacctgaac gaagtcgcgg 841 gcaagcatgg cgtgggccgt attgacatcg tggagaaccg cttcattgga atgaagtccc 901 gaggtatcta cgagacccca gcaggcacca tcctttacca cgctcattta gacatcgagg 961 ccttcaccat ggaccgggaa gtgcgcaaaa tcaaacaagg cctgggcttg aaatttgctg 1021 agctggtgta taccggtttc tggcacagcc ctgagtgtga atttgtccgc CACTGCATCG 1081 CCAAGTCCCA GGAGCGAGTG GAAGGGAAAG TGCAGGTGTC CGTCCTCAAG GGCCAGGTGT 1141 ACATCCTCGG CCGGGAGTCC CCACTGTCTC TCTACAATGA GGAGCTGGTG AGCATGAACG 1201 TGCAGGGTGA TTATGAGCCA ACTGATGCCA CCGGGTTCAT CAACATCAAT TCCCTCAGGC 1261 TGAAGGAATA TCATCGTCTC CAGAGCAAGG TCACTGCCAA ATACCCAACT TTCTTGTACA 1321 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1381 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1441 AGGACGAACT AATAACATTG GTCCGTTAAC GACGCGTTAA GTCgacaatc aacctctgga 1501 ttacaaaatt tgtgaaagat t