Construct: ORF TRCN0000465450
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018159.3_s317c1
- Derived from:
- ccsbBroadEn_14020
- DNA Barcode:
- CCGAAAGCAACTTGTTGGTTTCAC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SH3BP5 (9467)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465450
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9467 | SH3BP5 | SH3 domain binding protein 5 | XM_011534251.2 | 96.7% | 96.7% | (many diffs) |
| 2 | human | 9467 | SH3BP5 | SH3 domain binding protein 5 | XM_017007521.2 | 94.3% | 93.2% | (many diffs) |
| 3 | human | 9467 | SH3BP5 | SH3 domain binding protein 5 | NM_004844.5 | 93.4% | 93.4% | 1_90del |
| 4 | human | 9467 | SH3BP5 | SH3 domain binding protein 5 | NM_001018009.4 | 70.1% | 70.1% | 0_1ins381 |
| 5 | human | 9467 | SH3BP5 | SH3 domain binding protein 5 | XM_017007522.2 | 70.1% | 70.1% | 0_1ins381 |
| 6 | human | 9467 | SH3BP5 | SH3 domain binding protein 5 | XM_017007523.2 | 70.1% | 70.1% | 0_1ins381 |
| 7 | human | 9467 | SH3BP5 | SH3 domain binding protein 5 | XM_017007524.1 | 70.1% | 70.1% | 0_1ins381 |
| 8 | human | 9467 | SH3BP5 | SH3 domain binding protein 5 | XM_017007525.1 | 70.1% | 70.1% | 0_1ins381 |
| 9 | mouse | 24056 | Sh3bp5 | SH3-domain binding protein ... | NM_011894.3 | 82.6% | 87.1% | (many diffs) |
| 10 | mouse | 24056 | Sh3bp5 | SH3-domain binding protein ... | NM_001347585.1 | 79.2% | 77.7% | (many diffs) |
| 11 | mouse | 24056 | Sh3bp5 | SH3-domain binding protein ... | XM_006519015.2 | 62.2% | 64.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1341
- ORF length:
- 1275
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcaggggctg gaggaggaag aagaggtgga tccccggatc cagggagaac 121 tggagaagtt aaatcagtcc acggatgata tcaacagacg ggagactgaa cttgaggatg 181 ctcgtcagaa gttccgctct gttctggttg aagcaacggt gaaactggat gaactggtga 241 agaaaattgg caaagctgtg gaagactcca agccctactg ggaggcacgg agggtggcga 301 ggcaggctca gctggaagct cagaaagcca cgcaggactt ccagagggcc acagaggtgc 361 tccgtgccgc caaggagacc atctccctgg ccgagcagcg gctgctggag gatgacaagc 421 ggcagttcga ctccgcctgg caggagatgc tgaatcacgc cactcagagg gtcatggagg 481 cggagcagac caagaccagg agcgagctgg tgcataagga gacggcagcc aggtacaatg 541 ccgccatggg ccgcatgcga cagctggaga agaaactcaa gagagccatc aacaagtcca 601 agccttattt tgaactcaag gcaaagtact atgtgcagct cgagcaactg aaaaagactg 661 tggatgacct gcaggccaaa ctgaccctgg caaaaggcga gtacaagatg gccctgaaga 721 acctggagat gatctcagat gagatccacg agcggcggcg ctccagtgcc atggggcctc 781 ggggatgcgg tgttggtgct gagggcagca gcacatctgt ggaggatctg ccagggagca 841 aacctgagcc tgatgccatt tctgtggcct cggaggcctt tgaagatgac agctgtagca 901 actttgtgtc tgaagatgac tcggaaaccC AGTCCGTGTC CAGCTTTAGT TCAGGACCAA 961 CAAGCCCGTC TGAGATGCCT GACCAGTTCC CTGCGGTTGT GAGGCCTGGC AGCCTGGATC 1021 TGCCCAGCCC TGTGTCCCTG TCAGAGTTTG GGATGATGTT CCCAGTGTTG GGCCCTCGAA 1081 GTGAATGCAG CGGGGCCTCC TCCCCTGAAT GTGAAGTAGA ACGAGGAGAC AGGGCAGAAG 1141 GGGCAGAGAA TAAAACAAGT GACAAAGCCA ACAACAACCG GGGCCTCAGC AGTAGCAGTG 1201 GCAGTGGTGG CAGCAGTAAG AGCCAAAGCA GCACCTCCCC TGAGGGCCAG GCCTTGGAGA 1261 ACCGGATGAA GCAGCTCTCC CTACAGTGCT CAAAGGGAAG AGATGGAATT ATTGCTGACA 1321 TAAAAATGGT GCAGATTGGC TGCCCACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 1381 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 1441 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCGAA AGCAACTTGT 1501 TGGTTTCACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt