Construct: ORF TRCN0000465475
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000841.1_s317c1
- Derived from:
- ccsbBroadEn_13649
- DNA Barcode:
- AAGCAACCACGATGACCGTACCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM209B (388799)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465475
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 388799 | FAM209B | family with sequence simila... | NM_001013646.4 | 57.5% | 46.7% | (many diffs) |
| 2 | human | 200232 | FAM209A | family with sequence simila... | NM_001012971.3 | 56.2% | 45.3% | (many diffs) |
| 3 | human | 388799 | FAM209B | family with sequence simila... | XM_017027849.1 | 41.3% | 34.1% | (many diffs) |
| 4 | human | 200232 | FAM209A | family with sequence simila... | XR_001754199.1 | 30.3% | (many diffs) | |
| 5 | human | 388799 | FAM209B | family with sequence simila... | XR_001754273.1 | 13.1% | (many diffs) | |
| 6 | human | 388799 | FAM209B | family with sequence simila... | XR_001754272.1 | 12% | (many diffs) | |
| 7 | human | 388799 | FAM209B | family with sequence simila... | XR_001754271.1 | 9.7% | (many diffs) | |
| 8 | human | 388799 | FAM209B | family with sequence simila... | XR_001754270.1 | 7.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 384
- ORF length:
- 318
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGTG GACGCTGAAA TCGTCCCTGG TCCTGCTTCT GTGCCTCACC TGCAGCTATG 121 CCTTTATGTT CTCTTCTCTG AGACAGAAAA CTAGCGAACC CCAGGGGAAG GTGCCGTGTG 181 GAGAGCACTT TCGGATTCGG CAGAACCTAC CAGAGCACAC CCAAGGCTGG CTTGGGAGCA 241 AATGGCTCTG GCTTTTGTTT GCTGTTGTGC CGTTTGTGAT ACTGAAGTGT CAAAGAGACA 301 GTGAGAAGAA TAAGGTAAGG ATGGCTCCAT TTTTTTTACA CCATATTGAT TCAATCTCAG 361 GAGTCTCAGG GAAACGGATG TTCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 421 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 481 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA AGCAACCACG 541 ATGACCGTAC CTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 601 att