Construct: ORF TRCN0000465484
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010616.1_s317c1
- Derived from:
- ccsbBroadEn_06100
- DNA Barcode:
- CGGAAGATATTGTACATCACAGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DLST (1743)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465484
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1743 | DLST | dihydrolipoamide S-succinyl... | NM_001933.5 | 99.9% | 100% | 576G>A |
| 2 | human | 1743 | DLST | dihydrolipoamide S-succinyl... | XR_001750184.2 | 79.8% | (many diffs) | |
| 3 | human | 1743 | DLST | dihydrolipoamide S-succinyl... | NR_045209.2 | 46.5% | (many diffs) | |
| 4 | human | 1743 | DLST | dihydrolipoamide S-succinyl... | NR_033814.2 | 46.2% | (many diffs) | |
| 5 | human | 1744 | DLSTP1 | dihydrolipoamide S-succinyl... | NR_130749.1 | 43.2% | (many diffs) | |
| 6 | human | 1743 | DLST | dihydrolipoamide S-succinyl... | NM_001244883.2 | 33.7% | 32.7% | (many diffs) |
| 7 | mouse | 78920 | Dlst | dihydrolipoamide S-succinyl... | NM_030225.4 | 88.7% | 92% | (many diffs) |
| 8 | mouse | 78920 | Dlst | dihydrolipoamide S-succinyl... | XM_006516392.3 | 88.5% | 91.8% | (many diffs) |
| 9 | mouse | 78920 | Dlst | dihydrolipoamide S-succinyl... | XM_017315247.1 | 53.6% | 56.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1425
- ORF length:
- 1359
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gtcccgatcc cgctgtgtgt ctcgggcgtt cagccgctcg ctctccgcct 121 tccagaaggg gaactgccct ctagggagac gttccctgcc tggggtctcc ttatgccagg 181 gaccaggtta ccctaacagc aggaaggttg tcattaacaa cagtgtcttc agtgttcgct 241 ttttcagaac tacagctgta tgcaaggatg acttggttac agtcaaaacc ccagcgtttg 301 cagaatctgt cacagaggga gatgtcaggt gggagaaagc tgttggagac acagttgcag 361 aagatgaagt ggtttgtgag attgaaactg acaagacatc tgtgcaggtt ccatcaccag 421 caaatggcgt gattgaagct cttttggtac ctgatggggg aaaagtcgaa ggaggcactc 481 cacttttcac actcaggaaa actggtgctg ctcctgctaa ggccaagccg gctgaagctc 541 ctgctgctgc agccccaaaa gcagaaccta cagcagcggc agttcctccc cctgcagcac 601 ccatacccac tcagatgcca ccggtgccct cgccctcaca acctccttct ggcaaacctg 661 tgtctgcagt aaaacccact gttgccccac cactagctga gccaggagct ggcaaaggtc 721 tgcgttcaga acatcgggag aaaatgaaca ggatgcggca gcgcattgct cagcgtctga 781 aggaggccca gaatacatgt gcaatgctga caacttttaa tgagattgac atgagtaaca 841 tccaggagat gagggctcgg cacaaagagg cttttttgaa gaaacataac ctcaaactag 901 gcttcatgtc ggcatttgtg aaggcctcag cctttgcctt gcaggaacag cctgttgtaa 961 atgcagtgat tgacgacaca accaaagagg tggtgtatag ggattatatt gacatcagtg 1021 ttgcagtggc caccccacgg ggtctgGTGG TTCCAGTCAT CAGGAATGTG GAAGCTATGA 1081 ATTTTGCAGA TATTGAACGG ACCATCACTG AACTGGGAGA GAAGGCCCGA AAGAATGAAC 1141 TTGCCATTGA AGATATGGAT GGCGGTACCT TCACCATTAG CAATGGAGGC GTTTTTGGCT 1201 CGCTCTTTGG AACACCCATT ATCAACCCCC CTCAGTCTGC CATCCTGGGG ATGCATGGCA 1261 TCTTTGACAG GCCAGTGGCT ATAGGAGGCA AGGTAGAGGT GCGGCCCATG ATGTACGTGG 1321 CACTGACCTA TGATCACCGG CTGATTGATG GCAGAGAGGC TGTGACTTTC CTCCGCAAAA 1381 TCAAGGCAGC GGTAGAGGAT CCCAGAGTCC TCCTCCTGGA TCTTTACCCA ACTTTCTTGT 1441 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1501 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1561 GAAAGGACGA CGGAAGATAT TGTACATCAC AGATACGCGT TAAGTCgaca atcaacctct 1621 ggattacaaa atttgtgaaa gatt