Construct: ORF TRCN0000465638
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014130.1_s317c1
- Derived from:
- ccsbBroadEn_06179
- DNA Barcode:
- GCTAAATAAATTACTAAAGGACGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERG (2078)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465638
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2078 | ERG | ETS transcription factor ERG | NM_182918.4 | 99.9% | 100% | 552C>T |
| 2 | human | 2078 | ERG | ETS transcription factor ERG | NM_001136154.1 | 98.3% | 97.9% | (many diffs) |
| 3 | human | 2078 | ERG | ETS transcription factor ERG | NM_001243428.1 | 98.3% | 97.9% | (many diffs) |
| 4 | human | 2078 | ERG | ETS transcription factor ERG | NM_001331025.2 | 94.9% | 94.9% | 552C>T;670_671ins72 |
| 5 | human | 2078 | ERG | ETS transcription factor ERG | NM_004449.4 | 93.4% | 93% | (many diffs) |
| 6 | human | 2078 | ERG | ETS transcription factor ERG | NM_001136155.1 | 80.7% | 80.7% | 0_1ins276;276C>T |
| 7 | human | 2078 | ERG | ETS transcription factor ERG | NM_001243429.1 | 75.7% | 75.7% | 0_1ins276;276C>T;394_395ins72 |
| 8 | human | 2078 | ERG | ETS transcription factor ERG | NM_001243432.2 | 63.3% | 62.7% | (many diffs) |
| 9 | human | 2078 | ERG | ETS transcription factor ERG | NM_001291391.1 | 58.5% | 51.7% | (many diffs) |
| 10 | mouse | 13876 | Erg | ETS transcription factor | NM_001302153.1 | 90.1% | 97% | (many diffs) |
| 11 | mouse | 13876 | Erg | ETS transcription factor | NM_133659.3 | 88.6% | 95% | (many diffs) |
| 12 | mouse | 13876 | Erg | ETS transcription factor | XM_006522894.3 | 88.6% | 95% | (many diffs) |
| 13 | mouse | 13876 | Erg | ETS transcription factor | XM_006522895.2 | 88.6% | 95% | (many diffs) |
| 14 | mouse | 13876 | Erg | ETS transcription factor | XM_006522896.3 | 88.6% | 95% | (many diffs) |
| 15 | mouse | 13876 | Erg | ETS transcription factor | NM_001302154.1 | 85.8% | 92.6% | (many diffs) |
| 16 | mouse | 13876 | Erg | ETS transcription factor | NM_001302179.1 | 84.5% | 90.7% | (many diffs) |
| 17 | mouse | 13876 | Erg | ETS transcription factor | XM_006522899.3 | 84.5% | 90.7% | (many diffs) |
| 18 | mouse | 13876 | Erg | ETS transcription factor | XM_006522904.3 | 83.8% | 90.3% | (many diffs) |
| 19 | mouse | 13876 | Erg | ETS transcription factor | NM_001302152.1 | 83.8% | 90.1% | (many diffs) |
| 20 | mouse | 13876 | Erg | ETS transcription factor | XM_006522900.2 | 83.8% | 90.1% | (many diffs) |
| 21 | mouse | 13876 | Erg | ETS transcription factor | NM_001302183.1 | 79.6% | 85.8% | (many diffs) |
| 22 | mouse | 13876 | Erg | ETS transcription factor | XM_006522903.3 | 79.6% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1503
- ORF length:
- 1437
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagcactatt aaggaagcct tatcagttgt gagtgaggac cagtcgttgt 121 ttgagtgtgc ctacggaacg ccacacctgg ctaagacaga gatgaccgcg tcctcctcca 181 gcgactatgg acagacttcc aagatgagcc cacgcgtccc tcagcaggat tggctgtctc 241 aacccccagc cagggtcacc atcaaaatgg aatgtaaccc tagccaggtg aatggctcaa 301 ggaactctcc tgatgaatgc agtgtggcca aaggcgggaa gatggtgggc agcccagaca 361 ccgttgggat gaactacggc agctacatgg aggagaagca catgccaccc ccaaacatga 421 ccacgaacga gcgcagagtt atcgtgccag cagatcctac gctatggagt acagaccatg 481 tgcggcagtg gctggagtgg gcggtgaaag aatatggcct tccagacgtc aacatcttgt 541 tattccagaa catcgatggg aaggaactgt gcaagatgac caaggacgac ttccagaggc 601 tcacccccag ctacaatgcc gacatccttc tctcacatct ccactacctc agagagactc 661 ctcttccaca tttgacttca gatgatgttg ataaagcctt acaaaactct ccacggttaa 721 tgcatgctag aaacacaggg ggtgcagctt ttattttccc aaatacttca gtatatcctg 781 aagctacgca aagaattaca actaggccag atttaccata tgagcccccc aggagatcag 841 cctggaccgg tcacggccac cccacgcccc agtcgaaagc tgctcaacca tctccttcca 901 cagtgcccaa aactgaagac cagcgtcctc agttagatcc ttatcagatt cttggaccaa 961 caagtagccg ccttgcaaat ccaggcagtg gccagatcca gctttggcag ttcctcctgg 1021 agctcctgtc ggacagctcc aactccagct gcatcacctg ggaaggcacc aacggggagt 1081 tcaagatgac ggatcccgac gaggtggccc ggcgctgggg agagcggaag agcaaaccca 1141 acatgaacta cgataagcTC AGCCGCGCCC TCCGTTACTA CTATGACAAG AACATCATGA 1201 CCAAGGTCCA TGGGAAGCGC TACGCCTACA AGTTCGACTT CCACGGGATC GCCCAGGCCC 1261 TCCAGCCCCA CCCCCCGGAG TCATCTCTGT ACAAGTACCC CTCAGACCTC CCGTACATGG 1321 GCTCCTATCA CGCCCACCCA CAGAAGATGA ACTTTGTGGC GCCCCACCCT CCAGCCCTCC 1381 CCGTGACATC TTCCAGTTTT TTTGCTGCCC CAAACCCATA CTGGAATTCA CCAACTGGGG 1441 GTATATACCC CAACACTAGG CTCCCCACCA GCCATATGCC TTCTCATCTG GGCACTTACT 1501 ACTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1561 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1621 GGCTTTATAT ATCTTGTGGA AAGGACGAGC TAAATAAATT ACTAAAGGAC GCACGCGTTA 1681 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt