Construct: ORF TRCN0000465676
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000285.1_s317c1
- Derived from:
- ccsbBroadEn_11418
- DNA Barcode:
- TGCATATTGTGGGCGCGACGGAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GIT2 (9815)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465676
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 9815 | GIT2 | GIT ArfGAP 2 | NM_057170.5 | 86.3% | 86.3% | 1_258del |
2 | human | 9815 | GIT2 | GIT ArfGAP 2 | XM_006719712.4 | 80.8% | 80.8% | 1_258del;1238_1239ins105 |
3 | human | 9815 | GIT2 | GIT ArfGAP 2 | NM_014776.5 | 78.3% | 76.1% | (many diffs) |
4 | human | 9815 | GIT2 | GIT ArfGAP 2 | NM_001135213.3 | 78% | 75.7% | (many diffs) |
5 | human | 9815 | GIT2 | GIT ArfGAP 2 | XM_006719708.4 | 76.3% | 76.3% | 1_258del;1348_1596del |
6 | human | 9815 | GIT2 | GIT ArfGAP 2 | XM_017020258.2 | 76.1% | 76.1% | 1_258del;761_762insCAG;1345_1593del |
7 | human | 9815 | GIT2 | GIT ArfGAP 2 | XM_006719709.4 | 75.3% | 73.6% | (many diffs) |
8 | human | 9815 | GIT2 | GIT ArfGAP 2 | NM_001330153.2 | 75.2% | 73.4% | (many diffs) |
9 | human | 9815 | GIT2 | GIT ArfGAP 2 | NM_001135214.3 | 74.7% | 74.7% | 1_258del;1348_1641del |
10 | human | 9815 | GIT2 | GIT ArfGAP 2 | XM_006719707.4 | 73.2% | 73.2% | 1_258del;1347_1685del |
11 | human | 9815 | GIT2 | GIT ArfGAP 2 | NM_057169.5 | 71.8% | 71.8% | 1_258del;1347_1730del |
12 | human | 9815 | GIT2 | GIT ArfGAP 2 | XM_005253997.4 | 71.6% | 71.6% | 1_258del;761_762insCAG;1344_1727del |
13 | human | 9815 | GIT2 | GIT ArfGAP 2 | XM_017020260.2 | 65.5% | 65.4% | (many diffs) |
14 | human | 9815 | GIT2 | GIT ArfGAP 2 | XM_017020259.2 | 62.9% | 62.8% | (many diffs) |
15 | human | 9815 | GIT2 | GIT ArfGAP 2 | NM_139201.3 | 60% | 56.9% | (many diffs) |
16 | human | 9815 | GIT2 | GIT ArfGAP 2 | XM_017020261.2 | 59.8% | 56.8% | (many diffs) |
17 | human | 9815 | GIT2 | GIT ArfGAP 2 | XM_006719713.4 | 58% | 57.8% | (many diffs) |
18 | human | 9815 | GIT2 | GIT ArfGAP 2 | NM_001330154.2 | 52.9% | 52.2% | (many diffs) |
19 | mouse | 26431 | Git2 | G protein-coupled receptor ... | NM_001077360.1 | 69.1% | 70.9% | (many diffs) |
20 | mouse | 26431 | Git2 | G protein-coupled receptor ... | NM_001077359.1 | 68.9% | 70.5% | (many diffs) |
21 | mouse | 26431 | Git2 | G protein-coupled receptor ... | XM_006530338.3 | 68.9% | 70.6% | (many diffs) |
22 | mouse | 26431 | Git2 | G protein-coupled receptor ... | XM_017320893.1 | 66.3% | 68.1% | (many diffs) |
23 | mouse | 26431 | Git2 | G protein-coupled receptor ... | NM_019834.3 | 66.1% | 67.9% | (many diffs) |
24 | mouse | 26431 | Git2 | G protein-coupled receptor ... | XM_006530337.3 | 66.1% | 67.7% | (many diffs) |
25 | mouse | 26431 | Git2 | G protein-coupled receptor ... | XM_017320892.1 | 66.1% | 67.7% | (many diffs) |
26 | mouse | 26431 | Git2 | G protein-coupled receptor ... | XM_006530336.3 | 65.5% | 69.5% | (many diffs) |
27 | mouse | 26431 | Git2 | G protein-coupled receptor ... | XM_006530335.3 | 65.3% | 69.2% | (many diffs) |
28 | mouse | 26431 | Git2 | G protein-coupled receptor ... | XM_006530334.3 | 64% | 67.8% | (many diffs) |
29 | mouse | 26431 | Git2 | G protein-coupled receptor ... | NM_001347400.1 | 62.9% | 66.7% | (many diffs) |
30 | mouse | 26431 | Git2 | G protein-coupled receptor ... | XM_006530333.3 | 62.8% | 66.6% | (many diffs) |
31 | mouse | 26431 | Git2 | G protein-coupled receptor ... | XM_006530339.3 | 62.8% | 66.4% | (many diffs) |
32 | mouse | 26431 | Git2 | G protein-coupled receptor ... | XM_006530331.3 | 62.8% | 66.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1701
- ORF length:
- 1635
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tggaagacgt aaagctaatc cacaggataa agtacatccc aataaagcgg 121 aattcatcag agccaagtat cagatgttag cgttcgtcca tcgcttgccc tgccgggatg 181 acgatagtgt gactgccaaa gatcttagca agcaactcca ttcgagcgtg agaacaggga 241 atcttgaaac ctgtttgaga ctgttatctt taggagcaca agccaacttc tttcatcctg 301 aaaaaggaaa caccccactc catgttgcct ccaaagcagg gcagatttta caggctgaat 361 tattggcagt atatggagca gacccaggca cacaggattc tagtgggaaa actcccgttg 421 attatgcaag gcaaggaggg caccatgagc tggcagagcg cctcgtggaa atacagtatg 481 agctaacgga cagactagcc ttctatctct gtggcaggaa accagatcac aaaaatggac 541 agcactttat aatacctcaa atggcagaca gcagcctgga tttgtctgaa ttggcaaaag 601 ctgctaagaa gaaacttcaa tctctaagta atcatttgtt tgaagaactt gccatggatg 661 tgtacgatga agttgacagg cgagagacgg atgcagtctg gcttgccacg caaaaccaca 721 gcgccctggt aaccgagaca acggtcgtcc cctttcttcc ggtcaatcct gagtactcat 781 caacacgaaa tcagggcaga cagaagttag ctcggttcaa cgcccatgag tttgccacgc 841 tggtcattga cattctcagt gacgccaaga ggagacagca gggcagttct ctctcgggtt 901 caaaagacaa tgtggagctc atactgaaaa ccatcaataa ccagcacagc gttgagagtc 961 aagacaacga tcagcccgac tatgacagcg tggcatcaga cgaagacaca gatttggaaa 1021 ccactgcaag caaaacaaac cggcagaaga gcctagattc agatttatca gatggaccag 1081 tcactgtaca ggaatttatg gaggtcaaaa acgctctagt ggcttctgag gccaagatac 1141 agcagctaat gaaggcatcc aggctggaga agcagaacag cacacctgag agtgactacg 1201 acaacactcc caacgacatg gagccagatg gcatggggtc aagccgaaag ggacggcaaa 1261 gaagtatggt gtggccaggg gatggcttgg taccagacac agcagaaccc catgtggccc 1321 caagccccac tctccctagc accgaaGATG TCATCAGGAA GACTGAACAG ATCACCAAAA 1381 ACATACAGGA GCTCTTAAGA GCAGCCCAAG AAAATAAACA TGACAGTTAT ATTCCCTGCT 1441 CAGAGAGGAT ACACGTAGCT GTTACAGAAA TGGCAGCATT ATTCCCCAAA AAACCCAAGT 1501 CTGATATGGT GAGGACTTCC CTTCGTTTAC TGACGTCCAG TGCCTACCGA CTGCAGTCAG 1561 AGTGCAAGAA GACCCTCCCA GGGGACCCCG GCTCACCCAC AGACGTTCAG CTGGTCACGC 1621 AGCAGGTCAT CCAGTGTGCG TACGACATCG CCAAGGCTGC CAAGCAGCTG GTTACCATCA 1681 CCACCAAAGA GAACAACAAC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1741 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1801 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGCA TATTGTGGGC 1861 GCGACGGAGA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt