Construct: ORF TRCN0000465705
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011531.1_s317c1
- Derived from:
- ccsbBroadEn_09884
- DNA Barcode:
- CTCAGGCAAGTTTACGCTTGATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LHFPL5 (222662)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465705
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 222662 | LHFPL5 | LHFPL tetraspan subfamily m... | NM_182548.4 | 99.8% | 100% | 648C>A |
| 2 | mouse | 328789 | Lhfpl5 | lipoma HMGIC fusion partner... | NM_026571.2 | 89.4% | 98.1% | (many diffs) |
| 3 | mouse | 328789 | Lhfpl5 | lipoma HMGIC fusion partner... | XM_006524433.3 | 77.5% | 85.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 723
- ORF length:
- 657
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gaaattgctg ccggcccagg aggcagccaa gatctaccat accaactatg 121 tgcggaactc gcgagccgtg ggcgtgatgt ggggtaccct caccatctgc ttctccgtac 181 tggtcatggc cctcttcatc cagccctact ggatcggcga cagcgtcaac acaccgcagg 241 caggctactt cggccttttc tcctactgcg tgggtaacgt gctgtcctcc gagctcatct 301 gcaagggcgg ccccctagac ttctcctcca tcccctctag agccttcaag actgccatgt 361 tctttgtggc cttgggcatg ttcctcatca ttGGCTCCAT CATCTGCTTC AGCCTGTTCT 421 TCATCTGCAA CACGGCCACA GTCTATAAGA TCTGTGCATG GATGCAGCTG GCTGCGGCCA 481 CAGGCCTAAT GATTGGCTGC CTGGTCTACC CTGATGGTTG GGACTCAAGT GAGGTGCGGC 541 GCATGTGTGG GGAGCAGACG GGCAAGTACA CGCTGGGCCA CTGCACCATC CGCTGGGCCT 601 TCATGCTGGC CATCCTCAGC ATTGGCGACG CCCTCATCCT CTCCTTCCTG GCCTTCGTGT 661 TGGGCTACCG GCAGGACAAG CTCCTCCCTG ACGACTACAA GGCAGATGGA ACAGAGGAGG 721 TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 781 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 841 GGCTTTATAT ATCTTGTGGA AAGGACGACT CAGGCAAGTT TACGCTTGAT TTACGCGTTA 901 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt