Construct: ORF TRCN0000465895

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014333.1_s317c1
Derived from:
ccsbBroadEn_14986
DNA Barcode:
CCTGACATAGAGGTGGGACTACAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GUSBP15 (11042)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465895
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 102725009 LOC102725009 putative inactive beta-gluc... XM_017030249.2 86.1% 84.1% (many diffs)
2 human 375513 GUSBP4 GUSB pseudogene 4 NR_133000.1 72.3% (many diffs)
3 human 100049076 GUSBP9 GUSB pseudogene 9 NR_033968.1 68.5% (many diffs)
4 human 106660613 LINC00680-GUSBP4 LINC00680-GUSBP4 readthrough NR_132998.1 57.6% (many diffs)
5 human 728411 GUSBP1 GUSB pseudogene 1 NR_027028.2 56.1% (many diffs)
6 human 106660613 LINC00680-GUSBP4 LINC00680-GUSBP4 readthrough NR_003660.2 50.8% (many diffs)
7 human 106660613 LINC00680-GUSBP4 LINC00680-GUSBP4 readthrough NR_132997.1 37.7% (many diffs)
8 human 387036 GUSBP2 GUSB pseudogene 2 NR_003504.3 34.9% 1_347del;757C>G;768_1200del
9 human 11039 GUSBP14 GUSB pseudogene 14 NR_024054.2 34.8% 0_1ins49;372_1016del
10 human 2990 GUSB glucuronidase beta XM_017012093.2 31.4% 16.4% (many diffs)
11 human 2990 GUSB glucuronidase beta XM_017012092.1 29.3% 14.3% (many diffs)
12 human 2990 GUSB glucuronidase beta XM_017012091.1 27.7% 14.4% (many diffs)
13 human 2990 GUSB glucuronidase beta XM_011516114.2 27.7% 13.6% (many diffs)
14 human 2990 GUSB glucuronidase beta NM_001293105.2 27.7% 13.7% (many diffs)
15 human 653188 GUSBP3 GUSB pseudogene 3 NR_027386.2 27% 1_325del;746_1551del
16 human 2990 GUSB glucuronidase beta NM_001293104.2 26% 12% (many diffs)
17 human 2990 GUSB glucuronidase beta XM_011516113.1 24.8% 12.1% (many diffs)
18 human 11042 GUSBP15 GUSB pseudogene 15 NR_034021.1 23.7% (many diffs)
19 human 11039 GUSBP14 GUSB pseudogene 14 NR_029426.1 21.6% 1_243del;664_1943del
20 human 2990 GUSB glucuronidase beta XM_005250297.4 20% 10% (many diffs)
21 human 2990 GUSB glucuronidase beta NM_000181.4 18.5% 8.7% (many diffs)
22 human 2990 GUSB glucuronidase beta XR_001744660.2 17.9% (many diffs)
23 human 653080 GUSBP16 GUSB pseudogene 16 NR_146391.1 17.5% 1_386del;607C>A;807_2383del
24 human 2990 GUSB glucuronidase beta XR_927461.3 16.7% (many diffs)
25 human 114899 C1QTNF3 C1q and TNF related 3 NR_146599.1 9.1% (many diffs)
26 human 11039 GUSBP14 GUSB pseudogene 14 XR_002958912.1 7.8% (many diffs)
27 human 375513 GUSBP4 GUSB pseudogene 4 NR_132999.1 7.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
489
ORF length:
420
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggataggagc aaccctgtaa aaccagcctt agactatttt tcaaacaggc
121tggtgaatta ccagatctcc gtcaagtgca gtaacCAGTT CAAGTTGGAA GTGTGTCTTT
181TGAATGCAGA AAACAAAGTC GTGGACAACC AGGCTGGGAC CCAGGGCCAG CTGAAGGTGC
241TGGGTGCCAA CCTCTGGTGG CCGTACCTGA TGCACGAACA CCCCGCCTAC CTGTACTCGT
301GGGAGGATGG TGATTGCTCA CACCAAAGCC TTGGACCCCT CCCAGCCTGT GACCTTTGTG
361ACCAACTCCA CCTACGCAGC AGACAAGGGG GCTCTGTATG TGGATGTGAT CCGTGTGAAC
421AGCTACTACT CTTGGTATCG CAACTACGGG CACCTGGAGT TGATTCAGCT GCAGCTGGCC
481GCCCAGTTTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
541CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
601TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCTGAC ATAGAGGTGG GACTACAGAC
661GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)