Construct: ORF TRCN0000465904
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008365.1_s317c1
- Derived from:
- ccsbBroadEn_04573
- DNA Barcode:
- AGTACCGCGTCTGTCCTGGATTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS36 (92259)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465904
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92259 | MRPS36 | mitochondrial ribosomal pro... | NM_033281.6 | 100% | 100% | |
| 2 | mouse | 66128 | Mrps36 | mitochondrial ribosomal pro... | NM_025369.3 | 84.7% | 83.4% | (many diffs) |
| 3 | mouse | 66128 | Mrps36 | mitochondrial ribosomal pro... | NM_001190264.1 | 83.4% | 83.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 399
- ORF length:
- 309
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGATGGGCAG CAAGATGGCG TCTGCTAGTA 121 GGGTCGTTCA GGTAGTCAAA CCACACACTC CATTAATAAG GTTTCCTGAC AGAAGAGACA 181 ATCCTAAACC CAATGTATCA GAAGCTTTGA GATCAGCAGG GCTACCATCT CACTCTTCTG 241 TAATTTCACA ACATTCTAAA GGAAGTAAAT CACCAGATTT GCTGATGTAT CAGGGTCCAC 301 CAGACACTGC AGAAATAATA AAAACATTAC CTCAGAAATA CAGAAGGAAA CTTGTGTCTC 361 AAGAAGAAAT GGAATTTATC CAACGTGGAG GTCCTGAATA CCCAACTTTC TTGTACAAAG 421 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 481 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 541 ACGAAGTACC GCGTCTGTCC TGGATTCAAC GCGTTAAGTC gacaatcaac ctctggatta 601 caaaatttgt gaaagatt