Construct: ORF TRCN0000465946
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001019.1_s317c1
- Derived from:
- ccsbBroadEn_12422
- DNA Barcode:
- ATGACGTAGCCCATCTAACAAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EVA1C (59271)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465946
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 59271 | EVA1C | eva-1 homolog C | NM_001286556.2 | 99.9% | 99.7% | 56C>A |
| 2 | human | 59271 | EVA1C | eva-1 homolog C | NM_058187.5 | 99.2% | 99% | 56C>A;848_856delATGGTGAAT |
| 3 | human | 59271 | EVA1C | eva-1 homolog C | XM_017028418.1 | 99% | 98.8% | 56C>A;355_356insAGA;845_853delATGGTGAAT |
| 4 | human | 59271 | EVA1C | eva-1 homolog C | XM_006724038.3 | 99% | 98.6% | 56C>A;481_482insATG;845_853delATGGTGAAT |
| 5 | human | 59271 | EVA1C | eva-1 homolog C | XM_017028419.1 | 94.4% | 94.2% | 56C>A;776_777ins72 |
| 6 | human | 59271 | EVA1C | eva-1 homolog C | XM_005261019.4 | 87.5% | 87.4% | 56C>A;777_778ins162 |
| 7 | human | 59271 | EVA1C | eva-1 homolog C | XM_017028421.1 | 78.3% | 78.3% | 0_1ins285 |
| 8 | human | 59271 | EVA1C | eva-1 homolog C | XM_024452126.1 | 78% | 78% | 0_1ins285;70_71insAGA |
| 9 | human | 59271 | EVA1C | eva-1 homolog C | NM_001320745.2 | 77.7% | 77.7% | 0_1ins285;563_571delATGGTGAAT |
| 10 | human | 59271 | EVA1C | eva-1 homolog C | XM_011529669.3 | 77.7% | 77.7% | 0_1ins285;563_571delATGGTGAAT |
| 11 | human | 59271 | EVA1C | eva-1 homolog C | XM_017028420.1 | 77.7% | 77.7% | 0_1ins285;563_571delATGGTGAAT |
| 12 | human | 59271 | EVA1C | eva-1 homolog C | XM_024452125.1 | 77.5% | 77.5% | 0_1ins285;70_71insAGA;560_568delATGGTGAAT |
| 13 | human | 59271 | EVA1C | eva-1 homolog C | NR_135467.2 | 76.6% | (many diffs) | |
| 14 | human | 59271 | EVA1C | eva-1 homolog C | XM_017028422.1 | 75.4% | 75.1% | 56C>A;160_161ins321 |
| 15 | human | 59271 | EVA1C | eva-1 homolog C | NM_001320744.2 | 74.9% | 74.6% | 56C>A;160_161ins321;527_535delATGGTGAAT |
| 16 | human | 59271 | EVA1C | eva-1 homolog C | XM_017028423.1 | 70% | 69.6% | 56C>A;160_161ins321;455_456ins72 |
| 17 | human | 59271 | EVA1C | eva-1 homolog C | NR_104472.2 | 67.1% | (many diffs) | |
| 18 | human | 59271 | EVA1C | eva-1 homolog C | XR_001754885.1 | 34.3% | (many diffs) | |
| 19 | mouse | 70967 | Eva1c | eva-1 homolog C (C. elegans) | NM_001199210.2 | 85% | 85.9% | (many diffs) |
| 20 | mouse | 70967 | Eva1c | eva-1 homolog C (C. elegans) | XM_006523085.3 | 84.8% | 85.6% | (many diffs) |
| 21 | mouse | 70967 | Eva1c | eva-1 homolog C (C. elegans) | NM_027627.3 | 75.9% | 76.3% | (many diffs) |
| 22 | mouse | 70967 | Eva1c | eva-1 homolog C (C. elegans) | NM_001316760.1 | 66.1% | 66.5% | (many diffs) |
| 23 | mouse | 70967 | Eva1c | eva-1 homolog C (C. elegans) | XM_017317131.1 | 66.1% | 66.5% | (many diffs) |
| 24 | mouse | 70967 | Eva1c | eva-1 homolog C (C. elegans) | XM_017317132.1 | 66.1% | 66.5% | (many diffs) |
| 25 | mouse | 70967 | Eva1c | eva-1 homolog C (C. elegans) | NR_133573.1 | 40% | (many diffs) | |
| 26 | mouse | 70967 | Eva1c | eva-1 homolog C (C. elegans) | NR_133572.1 | 34.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1380
- ORF length:
- 1314
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct tctgccggga cgcgcacgcc aaccgccgac gccccagccc gtgcagcatc 121 acggcctccg ccggcaggta gagccgccgg ggcagctcct gcgcctcttc tactgcactg 181 tcctggtctg ctccaaagag atctcagcgc tcaccgactt ctctggttac ctaaccaaac 241 tcctgcaaaa ccacaccacc tatgcctgtg atggggacta tttgaatcta cagtgccctc 301 ggcattctac gataagtgtc caatcggcat tttatgggca agattaccaa atgtgtagtt 361 cccagaagcc tgcctcccag agggaagaca gcttaacctg tgtggcagcc accaccttcc 421 agaaggtgct ggacgaatgc cagaaccagc gggcctgcca cctcctggtc aatagccgtg 481 tttttggacc tgacctttgt ccaggaagca gtaaatacct cctggtctcc tttaaatgcc 541 aacctaatga attaaaaaac aaaaccgtgt gtgaagacca ggagctgaaa ctgcactgcc 601 atgaatccaa gttcctcaac atctactctg cgacctacgg caggaggacc caggaaaggg 661 acatctgctc ctccaaggca gagcggctcc cccctttcga ttgcttgtct tactcagctt 721 tgcaagtcct atcccgaagg tgctatggga agcagagatg caaaatcatc gtcaacaatc 781 accattttgg aagcccctgt ttgccaggcg tgaaaaaata cctcactgtg acctacgcat 841 gtgttcccaa gaacatactc acagcgattg atccagccat tgctaatcta aaaccttctt 901 tgaagcagaa agatggtata aacttcgacc caagcggatc gaaggttctg aggaaagatg 961 gaattcttgt tagcaactct ctggcagcct ttgcttacat tagagcccac ccggagagag 1021 ctgcccTGCT GTTCGTGTCC AGTGTCTGCA TCGGCCTGGC CCTCACACTG TGCGCCCTGG 1081 TCATCAGAGA GTCCTGTGCC AAGGACTTCC GCGACTTGCA GCTGGGGAGG GAGCAGCTGG 1141 TGCCAGGAAG TGACAAGGTC GAGGAGGACA GCGAGGATGA AGAAGAGGAG GAGGACCCCT 1201 CTGAGTCTGA TTTCCCAGGG GAACTGTCGG GGTTCTGTAG GACTTCATAT CCTATATACA 1261 GTTCCATAGA AGCTGCAGAG CTCGCAGAAA GGATTGAGCG CAGGGAGCAA ATCATTCAGG 1321 AAATATGGAT GAACAGTGGT TTGGACACCT CGCTCCCAAG AAACATGGGC CAGTTCTACT 1381 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1441 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1501 TTTATATATC TTGTGGAAAG GACGAATGAC GTAGCCCATC TAACAAATTA CGCGTTAAGT 1561 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt