Construct: ORF TRCN0000465965
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002995.1_s317c1
- Derived from:
- ccsbBroadEn_04559
- DNA Barcode:
- CTGTAATCCTACAAACCTTTAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YTHDC1 (91746)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465965
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91746 | YTHDC1 | YTH domain containing 1 | NM_001031732.4 | 99.9% | 100% | 198T>A |
| 2 | human | 91746 | YTHDC1 | YTH domain containing 1 | NM_001330698.2 | 98.8% | 98.9% | 198T>A;1718_1741del |
| 3 | human | 91746 | YTHDC1 | YTH domain containing 1 | NM_133370.4 | 97.4% | 97.5% | 198T>A;971_972ins54 |
| 4 | human | 91746 | YTHDC1 | YTH domain containing 1 | XM_005265708.3 | 96.4% | 96.4% | 198T>A;971_972ins54;1664_1687del |
| 5 | human | 91746 | YTHDC1 | YTH domain containing 1 | XM_017008832.2 | 95.4% | 94.1% | (many diffs) |
| 6 | human | 91746 | YTHDC1 | YTH domain containing 1 | XM_005265707.3 | 94.4% | 93.1% | (many diffs) |
| 7 | human | 91746 | YTHDC1 | YTH domain containing 1 | XM_017008833.2 | 92.9% | 91.7% | (many diffs) |
| 8 | human | 91746 | YTHDC1 | YTH domain containing 1 | XR_001741346.2 | 46.6% | (many diffs) | |
| 9 | human | 91746 | YTHDC1 | YTH domain containing 1 | XR_001741347.2 | 46.3% | (many diffs) | |
| 10 | human | 91746 | YTHDC1 | YTH domain containing 1 | XR_001741348.2 | 45.4% | (many diffs) | |
| 11 | mouse | 231386 | Ythdc1 | YTH domain containing 1 | NM_001347375.1 | 92.7% | 96.8% | (many diffs) |
| 12 | mouse | 231386 | Ythdc1 | YTH domain containing 1 | NM_177680.3 | 91.7% | 95.7% | (many diffs) |
| 13 | mouse | 231386 | Ythdc1 | YTH domain containing 1 | XM_006534864.1 | 91.2% | 95.2% | (many diffs) |
| 14 | mouse | 231386 | Ythdc1 | YTH domain containing 1 | NM_001347376.1 | 90.2% | 94.3% | (many diffs) |
| 15 | mouse | 231386 | Ythdc1 | YTH domain containing 1 | XM_006534863.2 | 90.2% | 94.2% | (many diffs) |
| 16 | mouse | 231386 | Ythdc1 | YTH domain containing 1 | XM_017320843.1 | 89.2% | 93.3% | (many diffs) |
| 17 | mouse | 231386 | Ythdc1 | YTH domain containing 1 | XM_006534866.1 | 88.7% | 92.8% | (many diffs) |
| 18 | mouse | 231386 | Ythdc1 | YTH domain containing 1 | XM_006534865.2 | 87.8% | 91.8% | (many diffs) |
| 19 | mouse | 231386 | Ythdc1 | YTH domain containing 1 | XM_006534867.3 | 82.8% | 84.8% | (many diffs) |
| 20 | mouse | 231386 | Ythdc1 | YTH domain containing 1 | XR_001784676.1 | 44.7% | (many diffs) | |
| 21 | mouse | 231386 | Ythdc1 | YTH domain containing 1 | XR_389269.3 | 41.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2247
- ORF length:
- 2181
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctgacagt cgggaggaga aagatggaga acttaatgtt ctggatgata 121 ttttaactga agtaccagaa caagatgatg aactgtataa tccagagagt gaacaagata 181 aaaatgagaa aaagggatca aaaagaaaaa gtgatcgaat ggaatctact gataccaaac 241 gacaaaagcc ttctgtccat tcaagacaac tggtttctaa gccactgagc tcatctgtta 301 gcaataacaa aagaatagtt agtacaaaag gaaagtcagc cacagagtat aaaaatgagg 361 aatatcaaag atctgaaaga aacaagcgtc tagatgctga tcggaaaatt cgtctatcaa 421 gtagtgcctc cagagaacct tataagaatc aacctgaaaa aacctgtgtc cggaaaaggg 481 atcctgaaag gagggccaaa tctcctacgc cagatggttc tgagagaatt gggcttgaag 541 tggatagacg tgcaagcaga tccagccagt cttctaagga agaagtgaac tctgaagaat 601 atggctctga ccatgagact ggcagcagtg gttcttctga tgagcaaggg aacaacactg 661 agaatgagga ggaaggagtg gaagaagatg tggaggaaga tgaagaagta gaagaagatg 721 cagaagaaga tgaagaggtg gatgaagatg gagaggagga ggaggaagag gaggaggagg 781 aagaggagga ggaggaggag gaagaagaag aatatgaaca ggatgagaga gaccagaaag 841 aggagggaaa tgattatgac actcgaagtg aggccagtga ctctggttct gaatctgttt 901 ccttcacaga tgggtctgtc agatctggtt caggcacaga tggatcagat gagaaaaaga 961 aggaaaggaa gagagctaga ggcatatctc caattgtttt tgatagaagt ggaagctctg 1021 catcagagtc atatgcaggt tcagaaaaga agcatgagaa attatcatct tccgttcgtg 1081 ctgtccgaaa agatcaaacc agtaaactca aatatgtgct tcaagatgca agatttttcc 1141 tcataaagag taacaaccat gagaatgtgt ctcttgccaa agcgaagggt gtatggtcca 1201 cgctccctgt aaatgagaag aaattaaatc ttgcatttag atctgcaagg agtgttatct 1261 taatattttc tgtcagagag agtggaaaat ttcaagggtt tgcaagactt tcttcagaat 1321 cacatcacgg aggatctcct atacactggg tgcttccagc aggaatgagt gctaaaatgc 1381 tgggaggtgt ctttaaaatt gactggattt gcaggcgtga attacccttc actaagtcgg 1441 ctcatctcac caatccttgg aatgaacata aaccagtaaa gatcggacgt gatggacagg 1501 aaattgaact tgaatgtgga acccagcttt gtcttctgtt tccccccgat gaaagtattg 1561 acttgtatca ggtcattcat aaaatgcgtc acaagagaag aatgcattct cagccccgat 1621 cacgaggacg tccatcccgt cgagaaccag tccgggatgt gggaaggcgt cgaccagaag 1681 attatgatat tcataacagc agaaagaaac caaggattga ctatccccct gagtttcacc 1741 agagaccagg gtatttaaag gatccacgat accaggaagt ggacagacga ttttcaggag 1801 ttcgccgaga tgtgttttta aatgggtcct acaatgatta tgtgagggaa tttcataaca 1861 tgggaccacc accaccttgg caaggaatgc ccccttaccc aggaatggaa caacctccac 1921 accatcctta ctatcagcac catgctccac ctcctcaagc tcaTCCCCCT TACTCAGGAC 1981 ATCATCCAGT ACCACATGAA GCAAGATACA GAGATAAACG AGTACATGAT TATGATATGA 2041 GGGTGGATGA TTTCCTTCGT CGCACACAAG CTGTTGTCAG TGGCCGGAGA AGTAGACCCC 2101 GTGAAAGAGA CCGGGAACGA GAGCGAGACC GCCCTAGAGA TAACAGACGA GACAGAGAGC 2161 GAGATAGAGG ACGTGATAGA GAAAGAGAAA GAGAGCGATT ATGTGATCGA GACAGAGACC 2221 GAGGGGAGAG AGGTCGATAT AGAAGATACC CAACTTTCTT GTACAAAGTG GTTGATATCG 2281 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2341 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACTGTAATC 2401 CTACAAACCT TTAATCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2461 aagatt