Construct: ORF TRCN0000465978
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017030.1_s317c1
- Derived from:
- ccsbBroadEn_15940
- DNA Barcode:
- CCCTACCGTCAGTTCATCCGTGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHTF2 (57157)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465978
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001127359.1 | 99.9% | 99.7% | 1049G>A |
| 2 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001127360.1 | 98.7% | 98.5% | 101_102insGTTTATTAAACT;1037G>A |
| 3 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366083.1 | 75.7% | 72.4% | (many diffs) |
| 4 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366085.2 | 72.2% | 69.1% | (many diffs) |
| 5 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366082.2 | 63% | 60.5% | (many diffs) |
| 6 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366088.2 | 63% | 60.5% | (many diffs) |
| 7 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_011516423.3 | 51.5% | 49.4% | (many diffs) |
| 8 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_017012459.2 | 50.9% | 48.9% | (many diffs) |
| 9 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_011516422.3 | 49.6% | 47.7% | (many diffs) |
| 10 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366081.1 | 49.6% | 47.7% | (many diffs) |
| 11 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_006716063.4 | 49.6% | 47.6% | (many diffs) |
| 12 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366084.1 | 49.1% | 47.1% | (many diffs) |
| 13 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366087.1 | 49.1% | 47.1% | (many diffs) |
| 14 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001127357.2 | 46.7% | 44.9% | (many diffs) |
| 15 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_005250508.5 | 46.7% | 44.9% | (many diffs) |
| 16 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_005250509.4 | 46.7% | 44.9% | (many diffs) |
| 17 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001127358.2 | 46.2% | 44.4% | (many diffs) |
| 18 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366086.1 | 46.2% | 44.4% | (many diffs) |
| 19 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_020432.5 | 46.2% | 44.4% | (many diffs) |
| 20 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366089.1 | 44.7% | 43% | (many diffs) |
| 21 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_011516424.2 | 28% | 26.3% | (many diffs) |
| 22 | mouse | 68770 | Phtf2 | putative homeodomain transc... | XM_006535804.3 | 83.6% | 84.5% | (many diffs) |
| 23 | mouse | 68770 | Phtf2 | putative homeodomain transc... | XM_006535801.1 | 45.5% | 43.8% | (many diffs) |
| 24 | mouse | 68770 | Phtf2 | putative homeodomain transc... | NM_172992.3 | 41.1% | 39.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1134
- ORF length:
- 1068
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtccaaagtc acagatgcta tagtctggta tcaaaagaag attggagcat 121 atgatcaaca aatatgggaa aaatctgttg aacagagaga aatcaagttt attaaactgg 181 ggctaaggaa taaaccaaag aaaacagcac atgtgaaacc agacctcata gatgttgatc 241 ttgtaagagg gtctgcattt gcaaaggcaa agcctgaaag tccttggact tctctgacca 301 gaaagggaat tgttcgagtt gtatttttcc cctttttctt ccggtggtgg ttacaagtaa 361 catcaaaggt catctttttc tggcttcttg tcctttatct tcttcaagtt gctgcaatag 421 tattattctg ctccacttct agcccacaca gcatacctct gacagaggtg attgggccga 481 tatggctgat gctgctcctg ggaactgtgc attgccagat tgtttccaca agaacaccca 541 aacctcctct aagtacaggg ggtaaaagaa gaaggaaatt aagaaaagca gcccatttgg 601 aagtacatag ggaaggagat ggttctagta ccacagataa cacacaagag ggagcagttc 661 agaaccacgg tacaagcacc tctcacagcg ttggcactgt cttcagagat ctctggcatg 721 ctgctttctt tttatcagga tcaaagaaag caaagaattc aattgataaa tcaactgaaa 781 ctgacaatGG CTATGTATCC CTTGATGGGA AGAAGACTGT TAAAAGCGGT GAAGATGGAA 841 TACAAAACCA TGAACCTCAG TGTGAAACTA TTCGACCAGA AGAGACAGCC TGGAACACAG 901 GAACACTGAG GAATGGTCCT AGCAAAGATA CCCAAAGGAC AATAACAAAT GTCTCTGATG 961 AAGTCTCCAG TGAGGAAGGT CCTGAAACAG GATACTCATT ACGTCGTCAT GTGGACAGGA 1021 CTTCTGAAGG TGTTCTTCGG AATAGAAAGT CACACCATTA TAAGAAACAT TACCCTAATG 1081 AGGTATATAC TTTGTCCTTA AGTGTATACA TACATATCTA TTTTATATGT TACTACCCAA 1141 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1201 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1261 TATCTTGTGG AAAGGACGAC CCTACCGTCA GTTCATCCGT GCGACGCGTT AAGTCgacaa 1321 tcaacctctg gattacaaaa tttgtgaaag att