Transcript: Human NM_001127358.2

Homo sapiens putative homeodomain transcription factor 2 (PHTF2), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-09
Taxon:
Homo sapiens (human)
Gene:
PHTF2 (57157)
Length:
5104
CDS:
235..2478

Additional Resources:

NCBI RefSeq record:
NM_001127358.2
NBCI Gene record:
PHTF2 (57157)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001127358.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000285225 ATAGAAAGTCACACCATTATA pLKO_005 1199 CDS 100% 15.000 10.500 N PHTF2 n/a
2 TRCN0000274460 TACTTGAGATCCTGGTAATTG pLKO_005 2734 3UTR 100% 13.200 9.240 N PHTF2 n/a
3 TRCN0000017181 CGATCAGTTGATGTAATAGTT pLKO.1 2008 CDS 100% 5.625 3.938 N PHTF2 n/a
4 TRCN0000017180 GCTAAGGAATAAACCAAAGAA pLKO.1 339 CDS 100% 5.625 3.938 N PHTF2 n/a
5 TRCN0000274404 GCTAAGGAATAAACCAAAGAA pLKO_005 339 CDS 100% 5.625 3.938 N PHTF2 n/a
6 TRCN0000017179 CCGGTTGAAGAAAGTACAGAA pLKO.1 1935 CDS 100% 4.950 3.465 N PHTF2 n/a
7 TRCN0000017178 GCCAGATTGTTTCCACAAGAA pLKO.1 671 CDS 100% 4.950 3.465 N PHTF2 n/a
8 TRCN0000274462 GCCAGATTGTTTCCACAAGAA pLKO_005 671 CDS 100% 4.950 3.465 N PHTF2 n/a
9 TRCN0000017182 GCCATATACCAGGAATAGGAT pLKO.1 1595 CDS 100% 3.000 2.100 N PHTF2 n/a
10 TRCN0000274463 GCCATATACCAGGAATAGGAT pLKO_005 1595 CDS 100% 3.000 2.100 N PHTF2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001127358.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03798 pDONR223 100% 99.4% 99.4% None 101_102insGTTTATTAAACT n/a
2 ccsbBroad304_03798 pLX_304 0% 99.4% 99.4% V5 101_102insGTTTATTAAACT n/a
3 TRCN0000474694 TTCCGATACCCTGCCCGTAGGATC pLX_317 19.9% 99.4% 99.4% V5 101_102insGTTTATTAAACT n/a
4 ccsbBroadEn_15941 pDONR223 0% 46.4% 44.6% None (many diffs) n/a
5 ccsbBroad304_15941 pLX_304 0% 46.4% 44.6% V5 (many diffs) n/a
6 TRCN0000472805 ATACAAACACTAAGAAAAATGACC pLX_317 21% 46.4% 44.6% V5 (many diffs) n/a
7 ccsbBroadEn_15940 pDONR223 0% 46.2% 44.4% None (many diffs) n/a
8 ccsbBroad304_15940 pLX_304 0% 46.2% 44.4% V5 (many diffs) n/a
9 TRCN0000465978 CCCTACCGTCAGTTCATCCGTGCG pLX_317 32.7% 46.2% 44.4% V5 (many diffs) n/a
10 ccsbBroadEn_15939 pDONR223 0% 42.3% 42.1% None (many diffs) n/a
11 ccsbBroad304_15939 pLX_304 0% 42.3% 42.1% V5 (many diffs) n/a
12 TRCN0000468673 ATTCAATCCGCAACACTGGTATCC pLX_317 48.1% 42.3% 42.1% V5 (many diffs) n/a
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