Construct: ORF TRCN0000465995
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008732.1_s317c1
- Derived from:
- ccsbBroadEn_10389
- DNA Barcode:
- TCCAACACTCCGTGGGTCTAGACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465995
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | NM_174919.3 | 24.7% | 23.1% | (many diffs) |
| 2 | human | 440461 | ARHGAP27P2 | Rho GTPase activating prote... | NR_027283.1 | 13% | 1_261del;478_1660del | |
| 3 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XM_011524473.2 | 11% | 10.3% | (many diffs) |
| 4 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XM_006721750.4 | 10.5% | 9.9% | (many diffs) |
| 5 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XM_017024313.1 | 10.3% | 9.6% | (many diffs) |
| 6 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XM_011524471.1 | 7.5% | 7% | (many diffs) |
| 7 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XM_006721747.2 | 7.4% | 7% | (many diffs) |
| 8 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XM_011524470.2 | 7.4% | 7% | (many diffs) |
| 9 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | NM_001282290.1 | 7.3% | 6.8% | (many diffs) |
| 10 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XM_006721745.2 | 7.3% | 6.8% | (many diffs) |
| 11 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XM_011524469.1 | 7.2% | 6.7% | (many diffs) |
| 12 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XM_011524468.1 | 7% | 6.6% | (many diffs) |
| 13 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XM_017024311.1 | 7% | 6.6% | (many diffs) |
| 14 | human | 146880 | ARHGAP27P1 | Rho GTPase activating prote... | NR_027487.2 | 6.8% | (many diffs) | |
| 15 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XR_429875.3 | 6.6% | (many diffs) | |
| 16 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XR_001752447.1 | 6.3% | (many diffs) | |
| 17 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XR_429876.3 | 6.3% | (many diffs) | |
| 18 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XR_001752448.1 | 6.2% | (many diffs) | |
| 19 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XR_429873.3 | 6.2% | (many diffs) | |
| 20 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XR_001752446.1 | 6.1% | (many diffs) | |
| 21 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XR_934416.2 | 4.2% | (many diffs) | |
| 22 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XR_934417.3 | 4.1% | (many diffs) | |
| 23 | human | 201176 | ARHGAP27 | Rho GTPase activating prote... | XR_002957982.1 | 3.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 306
- ORF length:
- 216
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCCGGCGTA TGTGGAGGGG GATGTCTACC 121 TGGTGGTGGA GCACCCCTTC GAGTATACCC GCAAGGACTG GCGCCGCGTG GCCATCTGGC 181 CCAATGAGCG CTACTGGCTG CTGCGGCGCA GCACGGAGCA CTGGTGGCAC GTGCGGCGCG 241 AGCCTGGCGG CCACCCCTTC TACCTGCCCG CGCAGTACGT GCGCGAGCTG CCTGCGCTGG 301 GCAAATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 361 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 421 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCCAACACT CCGTGGGTCT AGACTACGCG 481 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt