Construct: ORF TRCN0000466005
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000780.1_s317c1
- Derived from:
- ccsbBroadEn_11867
- DNA Barcode:
- CAACCGCAGTAGCTAGCACTAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCLO (27445)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466005
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27445 | PCLO | piccolo presynaptic cytomat... | XM_017012007.1 | 1.6% | 1.3% | 1_6561del;6660_6667delTGTAAGAC;6693_7683del |
| 2 | human | 27445 | PCLO | piccolo presynaptic cytomat... | XM_017012006.2 | 1.4% | 1.1% | 1_6561del;6660_6667delTGTAAGAC;6693_8514del |
| 3 | human | 27445 | PCLO | piccolo presynaptic cytomat... | NM_014510.3 | .8% | .6% | 1_13656del;13755_13762delTGTAAGAC;13788_14805del |
| 4 | human | 27445 | PCLO | piccolo presynaptic cytomat... | NM_033026.6 | .7% | .6% | 1_13656del;13755_13762delTGTAAGAC;13788_15426del |
| 5 | human | 27445 | PCLO | piccolo presynaptic cytomat... | XR_001744643.2 | .5% | 1_15225del;15324_15331delTGTAAGAC;15357_22942del | |
| 6 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | XM_006503586.3 | .8% | .8% | (many diffs) |
| 7 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | NM_001110796.2 | .7% | .6% | (many diffs) |
| 8 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | XM_006503584.3 | .7% | .6% | (many diffs) |
| 9 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | NM_011995.4 | .7% | .6% | (many diffs) |
| 10 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | XM_006503583.3 | .7% | .6% | (many diffs) |
| 11 | mouse | 26875 | Pclo | piccolo (presynaptic cytoma... | XM_006503582.3 | .7% | .6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 320
- ORF end:
- 443
- ORF length:
- 123
- Sequence:
-
1 TCAGCCGTCG CTTCATGTGA CTCCACGGAG TACCGGGCGC CGTCCAGGCA CCTCGATTAG 61 TTCTCGAGCT TTTGGAGTAC GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG 121 TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC 181 TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG 241 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG 301 TTTGTACAAA AAAGTTGGCA TGCAAGTATT GGAATGGAAT GGAATTCCCT TGACTTCTAA 361 AACATATGAA GAAGTTCAGA GTATCATTAG TCAGCAAAGT GGGGAAGCAG AAATATGTGG 421 ACCTCAATAT GCTATCAGAT TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA 481 GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT 541 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA ACCGCAGTAG 601 CTAGCACTAA CGACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 661 tt