Construct: ORF TRCN0000466022
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010533.1_s317c1
- Derived from:
- ccsbBroadEn_16065
- DNA Barcode:
- TTACCAAGGATTGAGGAACCATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR20 (91833)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466022
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91833 | WDR20 | WD repeat domain 20 | NM_001242415.2 | 100% | 100% | |
| 2 | human | 91833 | WDR20 | WD repeat domain 20 | XM_011537352.3 | 87.8% | 85.5% | (many diffs) |
| 3 | human | 91833 | WDR20 | WD repeat domain 20 | XM_011537353.3 | 85.9% | 85% | (many diffs) |
| 4 | human | 91833 | WDR20 | WD repeat domain 20 | NM_001320130.2 | 79.4% | 75.8% | (many diffs) |
| 5 | human | 91833 | WDR20 | WD repeat domain 20 | NM_001353679.2 | 79.4% | 75.3% | (many diffs) |
| 6 | human | 91833 | WDR20 | WD repeat domain 20 | XM_011537356.3 | 74.9% | 71.4% | (many diffs) |
| 7 | human | 91833 | WDR20 | WD repeat domain 20 | XM_011537358.3 | 71% | 69.5% | (many diffs) |
| 8 | human | 91833 | WDR20 | WD repeat domain 20 | NM_001353680.2 | 48.1% | 45.1% | (many diffs) |
| 9 | human | 91833 | WDR20 | WD repeat domain 20 | NM_001242414.2 | 43.5% | 42.5% | (many diffs) |
| 10 | human | 91833 | WDR20 | WD repeat domain 20 | NM_001353681.2 | 43.5% | 42.5% | (many diffs) |
| 11 | human | 91833 | WDR20 | WD repeat domain 20 | NM_144574.4 | 31.7% | 26.4% | (many diffs) |
| 12 | human | 91833 | WDR20 | WD repeat domain 20 | NM_001242418.2 | 31.1% | 25.9% | (many diffs) |
| 13 | human | 91833 | WDR20 | WD repeat domain 20 | NM_181291.3 | 31.1% | 25.9% | (many diffs) |
| 14 | human | 91833 | WDR20 | WD repeat domain 20 | NM_001242417.2 | 31% | 28.8% | (many diffs) |
| 15 | human | 91833 | WDR20 | WD repeat domain 20 | NM_001353657.2 | 30.9% | 25.8% | (many diffs) |
| 16 | human | 91833 | WDR20 | WD repeat domain 20 | NM_001353656.2 | 30.5% | 25.5% | (many diffs) |
| 17 | human | 91833 | WDR20 | WD repeat domain 20 | XM_017021767.1 | 30.4% | 25.4% | (many diffs) |
| 18 | human | 91833 | WDR20 | WD repeat domain 20 | NM_001330228.2 | 30.4% | 28.2% | (many diffs) |
| 19 | human | 91833 | WDR20 | WD repeat domain 20 | XM_011537340.3 | 30.3% | 28.1% | (many diffs) |
| 20 | human | 91833 | WDR20 | WD repeat domain 20 | XM_017021785.1 | 30.3% | 28.1% | (many diffs) |
| 21 | human | 91833 | WDR20 | WD repeat domain 20 | XM_011537339.2 | 30% | 27.9% | (many diffs) |
| 22 | human | 91833 | WDR20 | WD repeat domain 20 | XM_011537341.2 | 29.9% | 25% | (many diffs) |
| 23 | human | 91833 | WDR20 | WD repeat domain 20 | XM_011537338.1 | 29.9% | 27.8% | (many diffs) |
| 24 | human | 91833 | WDR20 | WD repeat domain 20 | XM_011537337.2 | 29.8% | 27.7% | (many diffs) |
| 25 | human | 91833 | WDR20 | WD repeat domain 20 | XM_011537336.2 | 29.7% | 27.6% | (many diffs) |
| 26 | human | 91833 | WDR20 | WD repeat domain 20 | XM_011537335.2 | 29.3% | 27.3% | (many diffs) |
| 27 | human | 91833 | WDR20 | WD repeat domain 20 | NR_038354.2 | 25.1% | (many diffs) | |
| 28 | human | 91833 | WDR20 | WD repeat domain 20 | XR_001750596.2 | 23% | (many diffs) | |
| 29 | human | 91833 | WDR20 | WD repeat domain 20 | XM_011537343.1 | 18.6% | 15.8% | (many diffs) |
| 30 | mouse | 69641 | Wdr20 | WD repeat domain 20 | NM_001347428.1 | 77.1% | 74.3% | (many diffs) |
| 31 | mouse | 69641 | Wdr20 | WD repeat domain 20 | NM_027149.3 | 31% | 26.1% | (many diffs) |
| 32 | mouse | 69641 | Wdr20 | WD repeat domain 20 | XM_006516204.3 | 30.4% | 25.5% | (many diffs) |
| 33 | mouse | 69641 | Wdr20 | WD repeat domain 20 | XM_006516203.3 | 30.2% | 25.4% | (many diffs) |
| 34 | mouse | 70948 | Wdr20rt | WD repeat domain 20, retrogene | NM_027614.1 | 30.1% | 26% | (many diffs) |
| 35 | mouse | 69641 | Wdr20 | WD repeat domain 20 | XR_872718.2 | 21.4% | (many diffs) | |
| 36 | mouse | 69641 | Wdr20 | WD repeat domain 20 | XR_001780476.1 | 15.9% | (many diffs) | |
| 37 | mouse | 69641 | Wdr20 | WD repeat domain 20 | XR_001780475.1 | 14.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 651
- ORF length:
- 585
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gacggaggga ggagggaagg agatgaacga gattaagacc caattcacca 121 cccgggaagg tctgtacaag ctgctgccgc actcggagta cagccggccc aaccgggtgc 181 ccttcaactc gcagggatcc aaccctgtcc gcgtctcctt cgtaaacctc aacgaccagt 241 ctggcaacgg cgaccgccTC TGCTTCAATG TGGGCCGGGA GCTGTACTTC TATATCTACA 301 AGGGGGTCCG CAAGGCTGCT GACTTGAGTA AACCAATAGA TAAAAGGATA TACAAAGGAA 361 CACAGCCTAC TTGTCATGAC TTCAACCACC TAACAGCCAC AGCAGAAAGT GTCTCTCTCC 421 TAGTGGGCTT TTCCGCAGGC CAAGTCCAGC TTATAGACCC AATCAAAAAA GAAACTAGCA 481 AACTTTTTAA TGAGGAAAAC TCTTGTCAGC ACTTGTGGAA GGTGGATTGG AATGAAGAAA 541 GACAGAATGA AGGTAGCAAG ACCAGTGAGG AGGCTCTAGT AACTGTCCAG CCGGCAGAGC 601 ACTTCTGCAG GCAGGAGGAC AGGATGCAAG GTGTTCTCCA AGACCAGAAC TACCCAACTT 661 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 721 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 781 CTTGTGGAAA GGACGATTAC CAAGGATTGA GGAACCATCT ACGCGTTAAG TCgacaatca 841 acctctggat tacaaaattt gtgaaagatt