Transcript: Mouse XR_001780476.1

PREDICTED: Mus musculus WD repeat domain 20 (Wdr20), transcript variant X9, misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Wdr20 (69641)
Length:
3337
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001780476.1
NBCI Gene record:
Wdr20 (69641)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_001780476.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000297624 CACGAGGAACCCTCTCCTTAA pLKO_005 1614 3UTR 100% 10.800 15.120 N Wdr20 n/a
2 TRCN0000279180 AGTGGGAACATGTACTTATAT pLKO_005 1501 3UTR 100% 15.000 10.500 N Wdr20 n/a
3 TRCN0000179270 GAGACCAGCAAGCTGTTTAAT pLKO.1 1396 3UTR 100% 15.000 10.500 N Wdr20 n/a
4 TRCN0000279113 TCAGTACACATGGGTATTAAT pLKO_005 3104 3UTR 100% 15.000 10.500 N Wdr20 n/a
5 TRCN0000134589 GCATGCCATGTAATTCTGAAT pLKO.1 2832 3UTR 100% 4.950 3.465 N WDR20 n/a
6 TRCN0000184353 GAAAGAGACCAGCAAGCTGTT pLKO.1 1392 3UTR 100% 4.050 2.835 N Wdr20 n/a
7 TRCN0000195851 CATGACTTCAACCTCCTCACA pLKO.1 1300 3UTR 100% 2.640 1.848 N Wdr20 n/a
8 TRCN0000279179 CTCAAGAGCAAGGACACATAC pLKO_005 2172 3UTR 100% 10.800 6.480 N Wdr20 n/a
9 TRCN0000134401 GAACGAGATTAAGACCCAATT pLKO.1 826 3UTR 100% 10.800 6.480 N WDR20 n/a
10 TRCN0000216627 GATAAACTGAACCTAGTTACT pLKO.1 2467 3UTR 100% 4.950 2.970 N Wdr20 n/a
11 TRCN0000183463 GCTCTACTTCTATATCTACAA pLKO.1 1012 3UTR 100% 4.950 2.970 N Wdr20 n/a
12 TRCN0000278905 GCTCTACTTCTATATCTACAA pLKO_005 1012 3UTR 100% 4.950 2.970 N Wdr20 n/a
13 TRCN0000184252 GTAAACCTCAACGACCAGTCT pLKO.1 953 3UTR 100% 2.640 1.584 N Wdr20 n/a
14 TRCN0000198907 GCTGCACCAGATCTAGAACTT pLKO.1 2702 3UTR 100% 4.950 2.475 Y Wdr20rt n/a
15 TRCN0000137293 GCACCAGATCTAGAACTTGAA pLKO.1 2705 3UTR 100% 4.950 2.475 Y WDR20 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001780476.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09322 pDONR223 100% 47.8% None (many diffs) n/a
2 ccsbBroad304_09322 pLX_304 0% 47.8% V5 (many diffs) n/a
3 TRCN0000471560 TTCCTTGAGCATGCGAAAGCAATC pLX_317 25.1% 47.8% V5 (many diffs) n/a
4 ccsbBroadEn_16065 pDONR223 0% 15.9% None (many diffs) n/a
5 ccsbBroad304_16065 pLX_304 0% 15.9% V5 (many diffs) n/a
6 TRCN0000466022 TTACCAAGGATTGAGGAACCATCT pLX_317 66.6% 15.9% V5 (many diffs) n/a
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