Transcript: Human XM_011537353.3

PREDICTED: Homo sapiens WD repeat domain 20 (WDR20), transcript variant X25, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
WDR20 (91833)
Length:
2294
CDS:
67..642

Additional Resources:

NCBI RefSeq record:
XM_011537353.3
NBCI Gene record:
WDR20 (91833)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011537353.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000136913 CGAGAAAGATCACAAGCGAAA pLKO.1 1358 3UTR 100% 4.050 5.670 N WDR20 n/a
2 TRCN0000138946 GTGTCACGTATCGGTTTGGTT pLKO.1 1027 3UTR 100% 3.000 4.200 N WDR20 n/a
3 TRCN0000138371 CGTAAGTGTCACGTATCGGTT pLKO.1 1022 3UTR 100% 2.640 3.696 N WDR20 n/a
4 TRCN0000137201 GCGCTCCTGTATTAGACTATT pLKO.1 1906 3UTR 100% 13.200 10.560 N WDR20 n/a
5 TRCN0000184252 GTAAACCTCAACGACCAGTCT pLKO.1 223 CDS 100% 2.640 2.112 N Wdr20 n/a
6 TRCN0000138658 CCTTGTTAGAGCCGCTGATAT pLKO.1 1498 3UTR 100% 13.200 9.240 N WDR20 n/a
7 TRCN0000134401 GAACGAGATTAAGACCCAATT pLKO.1 96 CDS 100% 10.800 7.560 N WDR20 n/a
8 TRCN0000136764 CAATGTGTGTTCAGCACAGAT pLKO.1 2050 3UTR 100% 4.950 3.465 N WDR20 n/a
9 TRCN0000137293 GCACCAGATCTAGAACTTGAA pLKO.1 1651 3UTR 100% 4.950 3.465 N WDR20 n/a
10 TRCN0000134589 GCATGCCATGTAATTCTGAAT pLKO.1 1786 3UTR 100% 4.950 3.465 N WDR20 n/a
11 TRCN0000198907 GCTGCACCAGATCTAGAACTT pLKO.1 1648 3UTR 100% 4.950 3.465 N Wdr20rt n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011537353.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_16065 pDONR223 0% 85.9% 85% None (many diffs) n/a
2 ccsbBroad304_16065 pLX_304 0% 85.9% 85% V5 (many diffs) n/a
3 TRCN0000466022 TTACCAAGGATTGAGGAACCATCT pLX_317 66.6% 85.9% 85% V5 (many diffs) n/a
4 ccsbBroadEn_09322 pDONR223 100% 29.6% 25.7% None (many diffs) n/a
5 ccsbBroad304_09322 pLX_304 0% 29.6% 25.7% V5 (many diffs) n/a
6 TRCN0000471560 TTCCTTGAGCATGCGAAAGCAATC pLX_317 25.1% 29.6% 25.7% V5 (many diffs) n/a
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