Construct: ORF TRCN0000466053

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011643.3_s317c1
Derived from:
ccsbBroadEn_13939
DNA Barcode:
ACTCATTGTGTTAAGAACACACCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MOK (5891)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466053
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5891 MOK MOK protein kinase XM_017021556.2 84.8% 84.9% 304_305insAAA;693_813delinsG
2 human 5891 MOK MOK protein kinase XM_006720226.3 78.8% 78.8% 304_305insAAA;694_876del
3 human 5891 MOK MOK protein kinase XM_006720225.3 78.5% 78.5% 304_305insAAA;694_879del
4 human 5891 MOK MOK protein kinase XM_017021555.2 78.2% 78.2% 304_305insAAA;409_410insAGC;691_876del
5 human 5891 MOK MOK protein kinase XM_017021557.2 68.6% 68.6% 122_123ins90;214_215insAAA;604_786del
6 human 5891 MOK MOK protein kinase XM_017021558.1 68.2% 68.2% (many diffs)
7 human 5891 MOK MOK protein kinase XM_011537052.2 64.7% 64.7% 304_305insAAA;694_1068del
8 human 5891 MOK MOK protein kinase NM_014226.3 55% 55% 304_305insAAA;694_1257del
9 human 5891 MOK MOK protein kinase NM_001330234.2 54.7% 54.7% 304_305insAAA;409_410insAGC;691_1254del
10 human 5891 MOK MOK protein kinase NM_001272011.2 47.8% 47.8% 122_123ins90;214_215insAAA;604_1167del
11 human 5891 MOK MOK protein kinase NM_001353827.2 47.6% 47.6% (many diffs)
12 human 5891 MOK MOK protein kinase XM_017021552.1 40.7% 39.8% (many diffs)
13 human 5891 MOK MOK protein kinase NM_001353828.2 39.7% 39.7% 0_1ins192;112_113insAAA;502_1065del
14 human 5891 MOK MOK protein kinase XM_024449681.1 39.2% 38.3% (many diffs)
15 human 5891 MOK MOK protein kinase XR_429323.3 38.5% 1_223del;527_528insAAA;917_1795del
16 human 5891 MOK MOK protein kinase XR_001750492.2 38.3% (many diffs)
17 human 5891 MOK MOK protein kinase XR_429320.3 37.7% 1_223del;527_528insAAA;917_1832del
18 human 5891 MOK MOK protein kinase XR_429322.3 36.3% 1_223del;527_528insAAA;917_1904del
19 human 5891 MOK MOK protein kinase NR_148552.2 33.5% (many diffs)
20 human 5891 MOK MOK protein kinase XR_943504.2 32.7% 1_223del;527_528insAAA;917_2111del
21 human 5891 MOK MOK protein kinase NM_001353829.2 30% 30% 0_1ins318;379_942del
22 human 5891 MOK MOK protein kinase NM_001353830.2 29.7% 29.7% 0_1ins318;94_95insAGC;376_939del
23 human 5891 MOK MOK protein kinase NR_148554.2 23.2% (many diffs)
24 human 5891 MOK MOK protein kinase NR_148553.2 21.9% (many diffs)
25 human 5891 MOK MOK protein kinase NR_148556.2 20.7% (many diffs)
26 human 5891 MOK MOK protein kinase NR_148555.2 19.3% (many diffs)
27 human 5891 MOK MOK protein kinase NM_001353831.2 11.6% 11.6% 0_1ins549;148_711del
28 mouse 26448 Mok MOK protein kinase XM_006515894.3 86.6% 93.2% (many diffs)
29 mouse 26448 Mok MOK protein kinase XM_006515889.2 56.8% 61.1% (many diffs)
30 mouse 26448 Mok MOK protein kinase XM_006515886.2 51% 54.9% (many diffs)
31 mouse 26448 Mok MOK protein kinase XM_006515885.2 49.2% 52.9% (many diffs)
32 mouse 26448 Mok MOK protein kinase NM_011973.2 49% 52.7% (many diffs)
33 mouse 26448 Mok MOK protein kinase XM_006515887.3 44.9% 48.4% (many diffs)
34 mouse 26448 Mok MOK protein kinase XM_006515893.2 33.6% 35.8% (many diffs)
35 mouse 26448 Mok MOK protein kinase XM_017315070.1 30.9% 33.4% (many diffs)
36 mouse 26448 Mok MOK protein kinase XM_017315071.1 30.9% 33.4% (many diffs)
37 mouse 26448 Mok MOK protein kinase XM_006515890.3 29.3% 31.1% (many diffs)
38 mouse 26448 Mok MOK protein kinase XM_006515891.2 28.9% 30.8% (many diffs)
39 mouse 26448 Mok MOK protein kinase XM_006515892.2 26.2% 28.7% (many diffs)
40 mouse 26448 Mok MOK protein kinase XM_011244115.2 26.2% 28.7% (many diffs)
41 mouse 26448 Mok MOK protein kinase XM_011244116.1 26.2% 28.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
762
ORF length:
696
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa gaactataaa gcaattggca aaataggaga gggaacgttt tctgaagtta
121tgaagatgca aagcctgaga gatggaaact actatgcatg taaacaaatg aagcagcgct
181ttgaaagtat tgagcaagtc aacaacctac gagagatcca agcactgagg cgcctgaatc
241cgcacccaaa cattcttatg ttgcatgaag tggtttttga cagaaaatct ggttctcttg
301cactaatatg tgaacttatg gacatgaata tttatgagct aatacgaggg agaagatacc
361cattatcaga aaaaaaaaaa attatgcact atatgtacca gttatgtaag tccctggatc
421atattcacag aaatggaata tttCACAGAG ATGTAAAACC AGAAAATATA CTAATAAAGC
481AGGATGTCCT GAAATTAGGG GACTTTGGCT CCTGCCGGAG TGTCTATTCC AAGCAGCCGT
541ACACGGAATA CATCTCCACC CGCTGGTACC GGGCCCCGGA GTGTCTCCTC ACTGATGGGT
601TCTACACGTA CAAGATGGAC CTGTGGAGCG CCGGCTGTGT GTTCTACGAG ATCGCCAGTC
661TGCAGCCCCT CTTTCCTGGA GTAAATGAAC TGGACCAAAT CTCAAAAATC CACGATGTCA
721TCGGCACACC CGCTCAGAAG ATCCTCACCA AGTTCAAACA GTACCCAACT TTCTTGTACA
781AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
841AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
901AGGACGAACT CATTGTGTTA AGAACACACC TACGCGTTAA GTCgacaatc aacctctgga
961ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)