Construct: ORF TRCN0000466101
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011545.1_s317c1
- Derived from:
- ccsbBroadEn_01399
- DNA Barcode:
- TAATCCGTTCGAACCTTCCAGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RLN1 (6013)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466101
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6013 | RLN1 | relaxin 1 | NM_006911.4 | 100% | 100% | |
| 2 | human | 6019 | RLN2 | relaxin 2 | NM_134441.3 | 90.4% | 82.1% | (many diffs) |
| 3 | human | 6013 | RLN1 | relaxin 1 | XM_024447637.1 | 81.6% | 73.5% | (many diffs) |
| 4 | human | 6019 | RLN2 | relaxin 2 | XM_024447646.1 | 81.6% | 73.5% | (many diffs) |
| 5 | human | 6019 | RLN2 | relaxin 2 | XM_024447647.1 | 81.6% | 73.5% | (many diffs) |
| 6 | human | 6013 | RLN1 | relaxin 1 | XM_024447638.1 | 77.1% | 70.2% | (many diffs) |
| 7 | human | 6013 | RLN1 | relaxin 1 | XM_017015007.1 | 70.2% | 61.6% | (many diffs) |
| 8 | human | 6013 | RLN1 | relaxin 1 | XM_017015008.1 | 66.1% | 63.6% | (many diffs) |
| 9 | human | 6013 | RLN1 | relaxin 1 | XM_017015009.1 | 55.7% | 41.5% | (many diffs) |
| 10 | human | 6013 | RLN1 | relaxin 1 | XM_024447642.1 | 53.5% | 53.5% | 0_1ins258 |
| 11 | human | 6013 | RLN1 | relaxin 1 | XM_024447643.1 | 53.5% | 53.5% | 0_1ins258 |
| 12 | human | 6013 | RLN1 | relaxin 1 | XM_024447644.1 | 53.5% | 53.5% | 0_1ins258 |
| 13 | human | 6013 | RLN1 | relaxin 1 | XM_024447645.1 | 53.5% | 53.5% | 0_1ins258 |
| 14 | human | 6013 | RLN1 | relaxin 1 | XM_017015010.1 | 50.9% | 39.2% | (many diffs) |
| 15 | human | 6019 | RLN2 | relaxin 2 | NM_001329191.1 | 46.9% | 41% | (many diffs) |
| 16 | human | 6013 | RLN1 | relaxin 1 | XM_024447639.1 | 46.9% | 41% | (many diffs) |
| 17 | human | 6013 | RLN1 | relaxin 1 | XM_024447640.1 | 46.9% | 41% | (many diffs) |
| 18 | human | 6013 | RLN1 | relaxin 1 | XM_024447641.1 | 46.9% | 41% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 621
- ORF length:
- 555
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tcgcctgttc ttgttccacc tgctagaatt ctgtttacta ctgaaccaat 121 tttccagagc agtcgcggcc aaatggaagg acgatgttat taaattatgc ggccgcgaat 181 tagttcgcgc gcagattgcc atttgcggca tgagcacctg gagcaaaagg tctctgagcc 241 aggaagatgc tcctcagaca cctagaccag tggcagaaat tgtaccaTCC TTCATCAACA 301 AAGATACAGA AACTATAATT ATCATGTTGG AATTCATTGC TAATTTGCCA CCGGAGCTGA 361 AGGCAGCCCT ATCTGAGAGG CAACCATCAT TACCAGAGCT ACAGCAGTAT GTACCTGCAT 421 TAAAGGATTC CAATCTTAGC TTTGAAGAAT TTAAGAAACT TATTCGCAAT AGGCAAAGTG 481 AAGCCGCAGA CAGCAATCCT TCAGAATTAA AATACTTAGG CTTGGATACT CATTCTCAAA 541 AAAAGAGACG ACCCTACGTG GCACTGTTTG AGAAATGTTG CCTAATTGGT TGTACCAAAA 601 GGTCTCTTGC TAAATATTGC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 661 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCCTTGAAAG 721 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATAATCCGT TCGAACCTTC 781 CAGGAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt