Construct: ORF TRCN0000466117
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011340.1_s317c1
- Derived from:
- ccsbBroadEn_11825
- DNA Barcode:
- CGATGCGGTCTTCATCTAAGGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LHX6 (26468)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466117
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26468 | LHX6 | LIM homeobox 6 | NM_001242334.2 | 100% | 100% | |
| 2 | human | 26468 | LHX6 | LIM homeobox 6 | NM_001348190.2 | 92.8% | 90.4% | (many diffs) |
| 3 | human | 26468 | LHX6 | LIM homeobox 6 | NM_014368.5 | 92.6% | 92.6% | 1_87del |
| 4 | human | 26468 | LHX6 | LIM homeobox 6 | XM_011518522.3 | 89.6% | 87.1% | (many diffs) |
| 5 | human | 26468 | LHX6 | LIM homeobox 6 | NM_001242333.2 | 88.5% | 86.1% | (many diffs) |
| 6 | human | 26468 | LHX6 | LIM homeobox 6 | NM_199160.4 | 86% | 83.7% | (many diffs) |
| 7 | human | 26468 | LHX6 | LIM homeobox 6 | XM_011518521.2 | 83.3% | 81% | (many diffs) |
| 8 | human | 26468 | LHX6 | LIM homeobox 6 | XM_005251916.3 | 51.1% | 50.7% | (many diffs) |
| 9 | human | 26468 | LHX6 | LIM homeobox 6 | NM_001242335.1 | 48.4% | 48.4% | 0_1ins561 |
| 10 | mouse | 16874 | Lhx6 | LIM homeobox protein 6 | NM_001083126.1 | 92.2% | 98.3% | (many diffs) |
| 11 | mouse | 16874 | Lhx6 | LIM homeobox protein 6 | XM_006497744.3 | 92.2% | 98.3% | (many diffs) |
| 12 | mouse | 16874 | Lhx6 | LIM homeobox protein 6 | NM_008500.2 | 85.4% | 91% | (many diffs) |
| 13 | mouse | 16874 | Lhx6 | LIM homeobox protein 6 | NM_001083127.1 | 84.5% | 88.8% | (many diffs) |
| 14 | mouse | 16874 | Lhx6 | LIM homeobox protein 6 | XM_017315869.1 | 84.5% | 88.8% | (many diffs) |
| 15 | mouse | 16874 | Lhx6 | LIM homeobox protein 6 | NM_001083125.1 | 78.4% | 82.3% | (many diffs) |
| 16 | mouse | 16874 | Lhx6 | LIM homeobox protein 6 | XR_374064.3 | 48.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1158
- ORF length:
- 1089
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcccagcca gggtccggct gcaaagcgac cacccgctgt cttgaaggga 121 ccgcgccgcc cgccatggct cagtctgacg ccgaggccct ggcaggagct ctggacaagg 181 acgagggtca ggcctcccca tgtacgccca gcacgccatc tgtctgctca ccgccctctg 241 ccgcctcctc cgtgccgtct gcaggcaaga acatctgctc cagctgcggc ctcgagatcc 301 tggaccgata tctgctcaag gtcaacaacc tcatctggca cgtgcggtgc ctcgagtgct 361 ccgtgtgtcg cacgtcgctg aggcagcaga acagctgcta catcaagaac aaggagatct 421 tctgcaagat ggactacttc agccgattcg ggaccaagtg tgcccggtgc ggccgacaga 481 tctacgccag cgactgggtg cggagagctc gcggcaacgc ctaccacctg gcctgcttcg 541 cctgcttctc gtgcaagcgc cagctgtcca ctggtgagga gttcggcctg gtcgaggaga 601 aggtgctctg ccgcatccac tacgacacca tgattgagaa cctcaagagg gccgccgaga 661 acgggaacgg cctcacgttg gagggggcag tgccctcgga acaggacagt caacccaagc 721 cggccaagcg cgcgcggacg tccttcaccg cggaacagct gcaggttatg caggcgcagt 781 tcgcgcagga caacaacccc gacgctcaga cgctgcagaa gctggcggac atgacgggcc 841 TCAGCCGGAG AGTCATCCAG GTGTGGTTTC AAAACTGCCG GGCGCGTCAT AAAAAGCACA 901 CGCCGCAACA CCCAGTGCCG CCCTCGGGGG CGCCCCCGTC CCGCCTTCCC TCCGCCCTGT 961 CCGACGACAT CCACTACACC CCGTTCAGCA GCCCCGAGCG GGCGCGCATG GTCACCCTGC 1021 ACGGCTACAT TGAGAGTCAG GTACAGTGCG GGCAGGTGCA CTGCCGGCTG CCTTACACCG 1081 CACCCCCCGT CCACCTCAAA GCCGATATGG ATGGGCCGCT CTCCAACCGG GGTGAGAAGG 1141 TCATCCTTTT TCAGTACTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1201 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1261 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACGATGCG GTCTTCATCT 1321 AAGGATCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt