Construct: ORF TRCN0000466123
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000171.1_s317c1
- Derived from:
- ccsbBroadEn_10679
- DNA Barcode:
- CATAATTGTGCCATGTGGCCTGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANXA4 (307)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466123
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 307 | ANXA4 | annexin A4 | NM_001320700.2 | 100% | 100% | |
| 2 | human | 307 | ANXA4 | annexin A4 | NM_001153.5 | 93.1% | 93.1% | 239_304del |
| 3 | human | 307 | ANXA4 | annexin A4 | NM_001320698.2 | 93.1% | 93.1% | 239_304del |
| 4 | human | 307 | ANXA4 | annexin A4 | NM_001365496.2 | 93.1% | 93.1% | 239_304del |
| 5 | human | 307 | ANXA4 | annexin A4 | XM_017003943.1 | 93.1% | 93.1% | 239_304del |
| 6 | human | 307 | ANXA4 | annexin A4 | XM_024452834.1 | 93.1% | 93.1% | 239_304del |
| 7 | human | 307 | ANXA4 | annexin A4 | XM_024452835.1 | 93.1% | 93.1% | 239_304del |
| 8 | human | 307 | ANXA4 | annexin A4 | NM_001320702.2 | 77.3% | 75.2% | (many diffs) |
| 9 | human | 307 | ANXA4 | annexin A4 | XM_017003944.1 | 77.3% | 75.2% | (many diffs) |
| 10 | human | 307 | ANXA4 | annexin A4 | XR_001738734.2 | 31.8% | (many diffs) | |
| 11 | mouse | 11746 | Anxa4 | annexin A4 | NM_001331120.1 | 79.9% | 85.3% | (many diffs) |
| 12 | mouse | 11746 | Anxa4 | annexin A4 | NM_013471.2 | 79.9% | 85.3% | (many diffs) |
| 13 | mouse | 11746 | Anxa4 | annexin A4 | XM_006505397.2 | 79.9% | 85.3% | (many diffs) |
| 14 | mouse | 11746 | Anxa4 | annexin A4 | XM_006505398.2 | 79.9% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 963
- ORF length:
- 897
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc catggcaacc aaaggaggta ctgtcaaagc tgcttcagga ttcaatgcca 121 tggaagatgc ccagaccctg aggaaggcca tgaaagggct cggcaccgat gaagacgcca 181 ttattagcgt ccttgcctac cgcaacaccg cccagcgcca ggagatcagg acagcctaca 241 agagcaccat cggcagggac ttgatagacg acctgaagtc agaactgagt ggcaacttcg 301 agcagggagc cggcactgat gagggctgcc taattgagat cctggcctcc cggacccctg 361 aggagatccg gcgcataagc caaacctacc agcagcaata tggacggagc cttgaagatg 421 acattcgctc tgacacatcg ttcatgttcc agcgagtgct ggtgtctctg tcagctggtg 481 ggagggatga aggaaattat ctggacgatg ctctcgtgag acaggatgcc caggacctgt 541 atgaggctGG AGAGAAGAAA TGGGGGACAG ATGAGGTGAA ATTTCTAACT GTTCTCTGTT 601 CCCGGAACCG AAATCACCTG TTGCATGTGT TTGATGAATA CAAAAGGATA TCACAGAAGG 661 ATATTGAACA GAGTATTAAA TCTGAAACAT CTGGTAGCTT TGAAGATGCT CTGCTGGCTA 721 TAGTAAAGTG CATGAGGAAC AAATCTGCAT ATTTTGCTGA AAAGCTCTAT AAATCGATGA 781 AGGGCTTGGG CACCGATGAT AACACCCTCA TCAGAGTGAT GGTTTCTCGA GCAGAAATTG 841 ACATGTTGGA TATCCGGGCA CACTTCAAGA GACTCTATGG AAAGTCTCTG TACTCGTTCA 901 TCAAGGGTGA CACATCTGGA GACTACAGGA AAGTACTGCT TGTTCTCTGT GGAGGAGATG 961 ATTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1021 TCGGTCTCGA TTCTACGTAG TAATGAACTA GCCCGTAACT TGAAAGTATT TCGATTTCTT 1081 GGCTTTATAT ATCTTGTGGA AAGGACGACA TAATTGTGCC ATGTGGCCTG CGACGCGTTA 1141 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt