Construct: ORF TRCN0000466199
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004705.1_s317c1
- Derived from:
- ccsbBroadEn_06884
- DNA Barcode:
- GAATTTGCATACAAAGTAATAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL23A (6147)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466199
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6147 | RPL23A | ribosomal protein L23a | NM_000984.6 | 99.7% | 100% | 462G>A |
| 2 | human | 56969 | RPL23AP32 | ribosomal protein L23a pseu... | NR_002229.2 | 67.6% | (many diffs) | |
| 3 | human | 285830 | HLA-F-AS1 | HLA-F antisense RNA 1 | NR_026973.1 | 60.8% | (many diffs) | |
| 4 | human | 649946 | RPL23AP64 | ribosomal protein L23a pseu... | NR_003040.2 | 58.6% | (many diffs) | |
| 5 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_000029.3 | 34.4% | (many diffs) | |
| 6 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_024529.1 | 30.5% | (many diffs) | |
| 7 | human | 284942 | RPL23AP82 | ribosomal protein L23a pseu... | NR_026982.1 | 29.2% | (many diffs) | |
| 8 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_024528.1 | 28.6% | (many diffs) | |
| 9 | human | 284942 | RPL23AP82 | ribosomal protein L23a pseu... | NR_026981.1 | 27.8% | (many diffs) | |
| 10 | human | 285830 | HLA-F-AS1 | HLA-F antisense RNA 1 | NR_026972.1 | 22.1% | (many diffs) | |
| 11 | human | 388574 | RPL23AP87 | ribosomal protein L23a pseu... | NR_029406.1 | 18.7% | (many diffs) | |
| 12 | human | 644128 | RPL23AP53 | ribosomal protein L23a pseu... | NR_003572.2 | 7.1% | (many diffs) | |
| 13 | mouse | 268449 | Rpl23a | ribosomal protein L23A | NM_207523.2 | 89.5% | 100% | (many diffs) |
| 14 | mouse | 108168233 | LOC108168233 | 60S ribosomal protein L23a ... | XR_001781614.1 | 73.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 534
- ORF length:
- 468
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gccgaaagcg aagaaggaag ctcctgcccc tcctaaagct gaagccaaag 121 cgaaggcttt aaaggccaag aaggcagtgt tgaaaggtgt ccacagccac aaaaagaaga 181 agatccgcac gtcacccacc ttccggcggc cgaagacact gcgactccgg agacagccca 241 aatatccTCG GAAGAGCGCT CCCAGGAGAA ACAAGCTTGA CCACTATGCT ATCATCAAGT 301 TTCCGCTGAC CACTGAGTCT GCCATGAAGA AGATAGAAGA CAACAACACA CTTGTGTTCA 361 TTGTGGATGT TAAAGCCAAC AAGCACCAGA TTAAACAGGC TGTGAAGAAG CTGTATGACA 421 TTGATGTGGC CAAGGTCAAC ACCCTGATTC GGCCTGATGG AGAGAAGAAG GCATATGTTC 481 GACTGGCTCC TGATTACGAT GCTTTGGATG TTGCCAACAA AATTGGAATC ATCTACCCAA 541 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 601 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 661 TATCTTGTGG AAAGGACGAG AATTTGCATA CAAAGTAATA GGCACGCGTT AAGTCgacaa 721 tcaacctctg gattacaaaa tttgtgaaag att