Construct: ORF TRCN0000466235
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011507.1_s317c1
- Derived from:
- ccsbBroadEn_13170
- DNA Barcode:
- TGCCGGTGGAACGACGATTACGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCLT1 (132320)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466235
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 132320 | SCLT1 | sodium channel and clathrin... | NM_001300897.2 | 100% | 100% | |
| 2 | human | 132320 | SCLT1 | sodium channel and clathrin... | NM_001300898.2 | 59.7% | 59.5% | 235_236delAAinsGC;238_393del |
| 3 | human | 132320 | SCLT1 | sodium channel and clathrin... | XM_017007718.2 | 11.9% | 11.8% | 235_236delAAinsGC;238_1971del |
| 4 | human | 132320 | SCLT1 | sodium channel and clathrin... | XM_017007717.2 | 11.4% | 11.4% | 235_236delAAinsGC;238_2052del |
| 5 | human | 132320 | SCLT1 | sodium channel and clathrin... | NM_144643.4 | 11.3% | 11.3% | 235_236delAAinsGC;238_2064del |
| 6 | human | 132320 | SCLT1 | sodium channel and clathrin... | XM_017007716.2 | 11.3% | 11.2% | 235_236delAAinsGC;238_2076del |
| 7 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_001741126.2 | 10.1% | 1_477del;712_713delAAinsGC;715_2318del | |
| 8 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_001741131.2 | 9.3% | 1_477del;712_713delAAinsGC;715_2505del | |
| 9 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_001741129.2 | 9.1% | 1_477del;712_713delAAinsGC;715_2576del | |
| 10 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_427528.3 | 8.2% | 1_477del;712_713delAAinsGC;715_2835del | |
| 11 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_001741128.2 | 8.2% | 1_477del;712_713delAAinsGC;715_2847del | |
| 12 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_001741130.2 | 8% | 1_477del;712_713delAAinsGC;715_2931del | |
| 13 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_002959708.1 | 7.7% | 1_477del;712_713delAAinsGC;715_3025del | |
| 14 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_001741127.2 | 7.4% | 1_477del;712_713delAAinsGC;715_3139del | |
| 15 | human | 132320 | SCLT1 | sodium channel and clathrin... | XR_938689.2 | 7.3% | 1_477del;712_713delAAinsGC;715_3218del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 327
- ORF length:
- 237
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCTGCAGA AATCGACTTT CTGAGAGAGC 121 AAAATCGAAG ACTAAATGAA GATTTTAGGC GGTATCAAAT GGAAAGTTTT TCCAAATATT 181 CATCTGTACA GAAAGCTGTC TGCCAAGGAG AAGGAGACGA CACATTTGAA AACCTAGTAT 241 TTGACCAAAG CTTTTTAGCT CCTCTTGTTA CTGAGTATGA TAAACACCTA GGAGAACTAA 301 ATGGGCAGCT GAAATATTAC CAGGCATACC CAACTTTCTT GTACAAAGTG GTTGATATCG 361 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 421 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATGCCGGTG 481 GAACGACGAT TACGGGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 541 aagatt