Construct: ORF TRCN0000466275
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006706.1_s317c1
- Derived from:
- ccsbBroadEn_08668
- DNA Barcode:
- GACAACTTTTGTCCTCCGTTCTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMCO4 (56935)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466275
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56935 | SMCO4 | single-pass membrane protei... | NM_020179.3 | 99.4% | 100% | 102G>A |
| 2 | human | 56935 | SMCO4 | single-pass membrane protei... | XM_011542911.2 | 35.5% | 1.3% | 1_178del;280G>A;356_495del |
| 3 | human | 56935 | SMCO4 | single-pass membrane protei... | XM_017018020.1 | 35.5% | 1.3% | 1_178del;280G>A;356_495del |
| 4 | human | 56935 | SMCO4 | single-pass membrane protei... | XM_017018019.2 | 31.5% | 2.2% | 1_241del;343G>A;419_558del |
| 5 | human | 56935 | SMCO4 | single-pass membrane protei... | XM_011542910.3 | 30.7% | 2.1% | 1_256del;358G>A;434_573del |
| 6 | human | 56935 | SMCO4 | single-pass membrane protei... | XM_011542909.1 | 30.2% | .8% | 1_265del;367G>A;443_582del |
| 7 | human | 56935 | SMCO4 | single-pass membrane protei... | XM_011542907.1 | 29.4% | .7% | 1_280del;382G>A;458_597del |
| 8 | mouse | 170748 | Smco4 | single-pass membrane protei... | NM_133214.2 | 88.7% | 96.6% | (many diffs) |
| 9 | mouse | 170748 | Smco4 | single-pass membrane protei... | XM_011242408.2 | 88.7% | 96.6% | (many diffs) |
| 10 | mouse | 170748 | Smco4 | single-pass membrane protei... | XM_011242409.2 | 88.7% | 96.6% | (many diffs) |
| 11 | mouse | 170748 | Smco4 | single-pass membrane protei... | XM_011242411.1 | 88.7% | 96.6% | (many diffs) |
| 12 | mouse | 170748 | Smco4 | single-pass membrane protei... | XM_006510043.2 | 31.7% | 34.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 142
- ORF end:
- 319
- ORF length:
- 177
- Sequence:
-
1 TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG 61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG CTAGCATCGA TTGATCAACA 121 AGTTTGTACA AAAAAGTTGG CATGCGGCAG CTCAAAGGGA AGCCCAAGAA GGAGACCTCC 181 AAGGACAAGA AGGAGCGGAA GCAAGCCATG CAGGAGGCCC GGCAGCAGAT CACTACAGTG 241 GTACTGCCCA CGCTGGCCGT GGTCGTGCTC TTGATCGTGG TGTTTGTGTA CGTGGCCACG 301 CGCCCCACCA TCACCGAGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT 361 ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA 421 AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGACAAC TTTTGTCCTC 481 CGTTCTGCAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt