Construct: ORF TRCN0000466327
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014653.1_s317c1
- Derived from:
- ccsbBroadEn_07484
- DNA Barcode:
- CTTCTATGCCATAGGGAACGGAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF144A (9781)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466327
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9781 | RNF144A | ring finger protein 144A | NM_001349182.2 | 99.8% | 99.6% | 10A>G |
| 2 | human | 9781 | RNF144A | ring finger protein 144A | NM_001349183.2 | 99.8% | 99.6% | 10A>G |
| 3 | human | 9781 | RNF144A | ring finger protein 144A | NM_001349184.1 | 99.8% | 99.6% | 10A>G |
| 4 | human | 9781 | RNF144A | ring finger protein 144A | NM_014746.6 | 99.8% | 99.6% | 10A>G |
| 5 | human | 9781 | RNF144A | ring finger protein 144A | XM_005246202.4 | 99.8% | 99.6% | 10A>G |
| 6 | human | 9781 | RNF144A | ring finger protein 144A | XM_017005401.2 | 87.7% | 82.8% | (many diffs) |
| 7 | human | 9781 | RNF144A | ring finger protein 144A | XM_017005402.2 | 87.7% | 82.8% | (many diffs) |
| 8 | human | 9781 | RNF144A | ring finger protein 144A | NM_001349185.2 | 86.5% | 85.9% | (many diffs) |
| 9 | human | 9781 | RNF144A | ring finger protein 144A | XM_017005398.2 | 86.5% | 85.9% | (many diffs) |
| 10 | human | 9781 | RNF144A | ring finger protein 144A | XM_017005400.2 | 86.5% | 85.9% | (many diffs) |
| 11 | human | 9781 | RNF144A | ring finger protein 144A | NM_001349181.2 | 85.2% | 77.7% | (many diffs) |
| 12 | human | 9781 | RNF144A | ring finger protein 144A | XM_017005403.2 | 85.1% | 84.9% | 10A>G;747_748ins129 |
| 13 | human | 9781 | RNF144A | ring finger protein 144A | NM_001349186.2 | 71.5% | 71.5% | 0_1ins249 |
| 14 | human | 9781 | RNF144A | ring finger protein 144A | XR_001739082.1 | 30.9% | (many diffs) | |
| 15 | mouse | 108089 | Rnf144a | ring finger protein 144A | NM_001081977.2 | 89.2% | 96.9% | (many diffs) |
| 16 | mouse | 108089 | Rnf144a | ring finger protein 144A | NM_080563.4 | 89.2% | 96.9% | (many diffs) |
| 17 | mouse | 108089 | Rnf144a | ring finger protein 144A | XM_006514948.3 | 89.2% | 96.9% | (many diffs) |
| 18 | mouse | 108089 | Rnf144a | ring finger protein 144A | XM_006514949.3 | 89.2% | 96.9% | (many diffs) |
| 19 | mouse | 108089 | Rnf144a | ring finger protein 144A | XM_011243807.2 | 89.2% | 96.9% | (many diffs) |
| 20 | mouse | 108089 | Rnf144a | ring finger protein 144A | XM_017314932.1 | 86.8% | 94.3% | (many diffs) |
| 21 | mouse | 108089 | Rnf144a | ring finger protein 144A | XM_017314933.1 | 86.8% | 94.3% | (many diffs) |
| 22 | mouse | 108089 | Rnf144a | ring finger protein 144A | XM_006514944.3 | 83.2% | 90.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 942
- ORF length:
- 876
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cacagcaagg taccggccca cctgggacct ggccctcgac ccgctggtgt 121 cttgcaagct ctgtcttggg gagtacccag tggagcagat gacaaccata gcccagtgcc 181 aatgcatctt ctgtactctg tgcctgaaac agtatgttga gctcttgatc aaagaaggat 241 tagaaaccgc aattagctgc ccagatgctg cctgccctaa acagggccac ctacaggaga 301 acgagattga gtgcatggtt gcagctgaaa ttatgcaaag atataaaaag ctacaatttg 361 aaagagaggt gctgtttgat ccctgtcgga cttggtgccc ggcgtccacc tgccaagctg 421 tgtgtcagct ccaggacgtg gggctgcaga ccccccagcc agtgcagtgc aaagcctgcc 481 gtatggaatt ctgctccacc tgcaaagcca gctggcaccc tggccagggc tgcccggaga 541 ccatgccgat caccttccTC CCCGGGGAGA CCAGTGCTGC TTTCAAAATG GAAGAAGATG 601 ACGCGCCCAT CAAGCGCTGC CCCAAGTGCA AAGTCTACAT CGAGCGAGAC GAAGGCTGCG 661 CGCAGATGAT GTGCAAGAAC TGCAAGCACG CCTTCTGCTG GTACTGCCTG GAGTCTCTGG 721 ACGATGATTT CCTTCTGATA CACTACGATA AGGGACCCTG CCGGAACAAG CTGGGCCACT 781 CCCGGGCATC TGTGATCTGG CATCGGACAC AGGTTGTGGG CATTTTTGCA GGATTTGGGC 841 TGCTGCTCTT GGTGGCCTCA CCTTTCCTAC TCCTGGCCAC TCCCTTTGTA CTTTGCTGCA 901 AGTGCAAGTG CAGTAAAGGT GACGACGACC CGTTACCCAC CTACCCAACT TTCTTGTACA 961 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1021 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1081 AGGACGACTT CTATGCCATA GGGAACGGAG TACGCGTTAA GTCgacaatc aacctctgga 1141 ttacaaaatt tgtgaaagat t