Construct: ORF TRCN0000466327

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014653.1_s317c1
Derived from:
ccsbBroadEn_07484
DNA Barcode:
CTTCTATGCCATAGGGAACGGAGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RNF144A (9781)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466327
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9781 RNF144A ring finger protein 144A NM_001349182.2 99.8% 99.6% 10A>G
2 human 9781 RNF144A ring finger protein 144A NM_001349183.2 99.8% 99.6% 10A>G
3 human 9781 RNF144A ring finger protein 144A NM_001349184.1 99.8% 99.6% 10A>G
4 human 9781 RNF144A ring finger protein 144A NM_014746.6 99.8% 99.6% 10A>G
5 human 9781 RNF144A ring finger protein 144A XM_005246202.4 99.8% 99.6% 10A>G
6 human 9781 RNF144A ring finger protein 144A XM_017005401.2 87.7% 82.8% (many diffs)
7 human 9781 RNF144A ring finger protein 144A XM_017005402.2 87.7% 82.8% (many diffs)
8 human 9781 RNF144A ring finger protein 144A NM_001349185.2 86.5% 85.9% (many diffs)
9 human 9781 RNF144A ring finger protein 144A XM_017005398.2 86.5% 85.9% (many diffs)
10 human 9781 RNF144A ring finger protein 144A XM_017005400.2 86.5% 85.9% (many diffs)
11 human 9781 RNF144A ring finger protein 144A NM_001349181.2 85.2% 77.7% (many diffs)
12 human 9781 RNF144A ring finger protein 144A XM_017005403.2 85.1% 84.9% 10A>G;747_748ins129
13 human 9781 RNF144A ring finger protein 144A NM_001349186.2 71.5% 71.5% 0_1ins249
14 human 9781 RNF144A ring finger protein 144A XR_001739082.1 30.9% (many diffs)
15 mouse 108089 Rnf144a ring finger protein 144A NM_001081977.2 89.2% 96.9% (many diffs)
16 mouse 108089 Rnf144a ring finger protein 144A NM_080563.4 89.2% 96.9% (many diffs)
17 mouse 108089 Rnf144a ring finger protein 144A XM_006514948.3 89.2% 96.9% (many diffs)
18 mouse 108089 Rnf144a ring finger protein 144A XM_006514949.3 89.2% 96.9% (many diffs)
19 mouse 108089 Rnf144a ring finger protein 144A XM_011243807.2 89.2% 96.9% (many diffs)
20 mouse 108089 Rnf144a ring finger protein 144A XM_017314932.1 86.8% 94.3% (many diffs)
21 mouse 108089 Rnf144a ring finger protein 144A XM_017314933.1 86.8% 94.3% (many diffs)
22 mouse 108089 Rnf144a ring finger protein 144A XM_006514944.3 83.2% 90.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
942
ORF length:
876
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac cacagcaagg taccggccca cctgggacct ggccctcgac ccgctggtgt
121cttgcaagct ctgtcttggg gagtacccag tggagcagat gacaaccata gcccagtgcc
181aatgcatctt ctgtactctg tgcctgaaac agtatgttga gctcttgatc aaagaaggat
241tagaaaccgc aattagctgc ccagatgctg cctgccctaa acagggccac ctacaggaga
301acgagattga gtgcatggtt gcagctgaaa ttatgcaaag atataaaaag ctacaatttg
361aaagagaggt gctgtttgat ccctgtcgga cttggtgccc ggcgtccacc tgccaagctg
421tgtgtcagct ccaggacgtg gggctgcaga ccccccagcc agtgcagtgc aaagcctgcc
481gtatggaatt ctgctccacc tgcaaagcca gctggcaccc tggccagggc tgcccggaga
541ccatgccgat caccttccTC CCCGGGGAGA CCAGTGCTGC TTTCAAAATG GAAGAAGATG
601ACGCGCCCAT CAAGCGCTGC CCCAAGTGCA AAGTCTACAT CGAGCGAGAC GAAGGCTGCG
661CGCAGATGAT GTGCAAGAAC TGCAAGCACG CCTTCTGCTG GTACTGCCTG GAGTCTCTGG
721ACGATGATTT CCTTCTGATA CACTACGATA AGGGACCCTG CCGGAACAAG CTGGGCCACT
781CCCGGGCATC TGTGATCTGG CATCGGACAC AGGTTGTGGG CATTTTTGCA GGATTTGGGC
841TGCTGCTCTT GGTGGCCTCA CCTTTCCTAC TCCTGGCCAC TCCCTTTGTA CTTTGCTGCA
901AGTGCAAGTG CAGTAAAGGT GACGACGACC CGTTACCCAC CTACCCAACT TTCTTGTACA
961AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1021AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1081AGGACGACTT CTATGCCATA GGGAACGGAG TACGCGTTAA GTCgacaatc aacctctgga
1141ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)