Construct: ORF TRCN0000466412
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008695.1_s317c1
- Derived from:
- ccsbBroadEn_02686
- DNA Barcode:
- AAACCCCAGAACTGAAAACCACAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COPZ1 (22818)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466412
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22818 | COPZ1 | COPI coat complex subunit z... | NM_016057.3 | 100% | 100% | |
| 2 | human | 22818 | COPZ1 | COPI coat complex subunit z... | NM_001271736.2 | 95.1% | 92.9% | (many diffs) |
| 3 | human | 22818 | COPZ1 | COPI coat complex subunit z... | NM_001271735.2 | 90.3% | 90.3% | 434_435ins51 |
| 4 | human | 22818 | COPZ1 | COPI coat complex subunit z... | NM_001271734.2 | 87% | 87% | 17_18ins69 |
| 5 | human | 22818 | COPZ1 | COPI coat complex subunit z... | NR_073424.2 | 23.2% | 1_33del;202_203ins92;473_1798del | |
| 6 | human | 22818 | COPZ1 | COPI coat complex subunit z... | XR_002957300.1 | 14.7% | (many diffs) | |
| 7 | mouse | 56447 | Copz1 | coatomer protein complex, s... | NM_019817.1 | 93.2% | 100% | (many diffs) |
| 8 | mouse | 56447 | Copz1 | coatomer protein complex, s... | XM_006521211.2 | 83.9% | 87.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 597
- ORF length:
- 531
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggcgctgatt ttggaacctt ccctgtatac tgtcaaagcc atcctgattc 121 tggacaatga tggagatcga ctttttgcca agtactatga cgacacctac cccagtgtca 181 aggagcaaaa ggccttTGAG AAGAACATTT TCAACAAGAC CCATCGGACT GACAGTGAAA 241 TTGCCCTCTT GGAAGGCCTG ACAGTGGTAT ACAAAAGCAG TATAGATCTC TATTTCTATG 301 TGATTGGCAG CTCCTATGAA AATGAGCTGA TGCTTATGGC TGTTCTGAAC TGTCTCTTCG 361 ACTCATTGAG CCAGATGCTG AGGAAAAATG TAGAAAAGCG AGCACTGCTG GAGAACATGG 421 AGGGGCTGTT CTTGGCTGTG GATGAAATTG TAGATGGAGG GGTGATCCTA GAGAGTGATC 481 CCCAGCAGGT GGTACACCGG GTGGCATTAA GGGGTGAAGA TGTCCCCCTT ACGGAGCAGA 541 CCGTGTCTCA GGTGCTGCAG TCAGCCAAAG AACAGATCAA GTGGTCACTC CTTCGGTGCC 601 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 661 TCGATTCTAC GTAGTAATGA ACTAGCCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 721 ATATATCTTG TGGAAAGGAC GAAAACCCCA GAACTGAAAA CCACAGACGC GTTAAGTCga 781 caatcaacct ctggattaca aaatttgtga aagatt