Construct: ORF TRCN0000466446
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002968.3_s317c1
- Derived from:
- ccsbBroadEn_02123
- DNA Barcode:
- TCTTTACTTTACGTCATCATTACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS6KA5 (9252)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466446
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9252 | RPS6KA5 | ribosomal protein S6 kinase A5 | NM_182398.2 | 100% | 100% | |
| 2 | human | 9252 | RPS6KA5 | ribosomal protein S6 kinase A5 | NM_001322229.2 | 69% | 68.9% | 1645_1648delAATTinsG;1651_2385del |
| 3 | human | 9252 | RPS6KA5 | ribosomal protein S6 kinase A5 | NM_004755.4 | 68.4% | 68.3% | 1645_1648delAATTinsG;1651_2406del |
| 4 | human | 9252 | RPS6KA5 | ribosomal protein S6 kinase A5 | NM_001322236.2 | 64.9% | 64.8% | 618_619ins84;1561_1564delAATTinsG;1567_2322del |
| 5 | human | 9252 | RPS6KA5 | ribosomal protein S6 kinase A5 | NM_001322228.2 | 63.9% | 63.8% | 510_511ins108;1537_1540delAATTinsG;1543_2298del |
| 6 | human | 9252 | RPS6KA5 | ribosomal protein S6 kinase A5 | NM_001322233.2 | 61.6% | 61.5% | 951_952ins162;1483_1486delAATTinsG;1489_2244del |
| 7 | human | 9252 | RPS6KA5 | ribosomal protein S6 kinase A5 | NM_001322232.2 | 59.3% | 59.2% | 173_174ins219;1426_1429delAATTinsG;1432_2187del |
| 8 | human | 9252 | RPS6KA5 | ribosomal protein S6 kinase A5 | NM_001322235.2 | 58.5% | 58.4% | 0_1ins237;1408_1411delAATTinsG;1414_2169del |
| 9 | human | 9252 | RPS6KA5 | ribosomal protein S6 kinase A5 | NM_001322237.2 | 58.5% | 58.4% | 0_1ins237;1408_1411delAATTinsG;1414_2169del |
| 10 | human | 9252 | RPS6KA5 | ribosomal protein S6 kinase A5 | NM_001322234.2 | 44.1% | 43.3% | (many diffs) |
| 11 | human | 9252 | RPS6KA5 | ribosomal protein S6 kinase A5 | NM_001322238.2 | 41.1% | 41% | 0_1ins657;988_991delAATTinsG;994_1749del |
| 12 | human | 9252 | RPS6KA5 | ribosomal protein S6 kinase A5 | XM_017021786.2 | 41.1% | 41% | 0_1ins657;988_991delAATTinsG;994_1749del |
| 13 | human | 9252 | RPS6KA5 | ribosomal protein S6 kinase A5 | NM_001322231.2 | 38.6% | 35.3% | (many diffs) |
| 14 | human | 9252 | RPS6KA5 | ribosomal protein S6 kinase A5 | NM_001322230.2 | 35% | 34.9% | (many diffs) |
| 15 | human | 9252 | RPS6KA5 | ribosomal protein S6 kinase A5 | NM_001322227.2 | 33.3% | 33.2% | 0_1ins843;802_805delAATTinsG;808_1563del |
| 16 | mouse | 73086 | Rps6ka5 | ribosomal protein S6 kinase... | NM_001330702.1 | 62.1% | 66.5% | (many diffs) |
| 17 | mouse | 73086 | Rps6ka5 | ribosomal protein S6 kinase... | XM_006516275.3 | 59.5% | 63.5% | (many diffs) |
| 18 | mouse | 73086 | Rps6ka5 | ribosomal protein S6 kinase... | NM_153587.3 | 57.4% | 61.5% | (many diffs) |
| 19 | mouse | 73086 | Rps6ka5 | ribosomal protein S6 kinase... | XM_006516276.3 | 37.1% | 39.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1713
- ORF length:
- 1647
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaggagggt ggcagcagcg gcggcgccgc ggggaccagc gcggacggcg 121 gcgacggagg agagcagctc ctcactgtca agcacgagct gcggactgct aatttgacag 181 gacatgctga gaaggtggga atagaaaatt ttgagctcct gaaggtccta ggaactggag 241 cttatggaaa agtatttcta gttcgtaaaa taagtggcca tgatactgga aagctgtatg 301 ccatgaaagt tttgaaaaag gcaacaatcg ttcaaaaggc caaaaccaca gagcatacaa 361 ggacagaacg acaagtcctg gaacacatta ggcagtcgcc atttttggta acattacatt 421 atgctttcca gacagaaacc aaacttcatc tcattttaga ttatataaat ggtggtgaac 481 tttttactca tctttctcaa agagagcgtt tcacagagca tgaggtgcag atttatgttg 541 gagagattgt gcttgccctc gaacatctcc acaagttggg gattatatat cgtgatatta 601 agcttgagaa tattctactt gattctaatg gccatgtggt gctgacagat tttggtctga 661 gtaaggagtt tgtggctgat gaaactgaaa gagcatattc cttttgtgga actattgaat 721 acatggcacc agatattgtc agagggggag attcaggaca tgacaaggca gttgactggt 781 ggagtttggg tgttctaatg tatgaattac taactggagc atctcctttc actgttgatg 841 gagaaaaaaa ttcccaagct gagatatcta ggagaatatt aaaaagtgag cctccatatc 901 cccaagaaat gagtgcttta gcgaaagacc taattcagcg tcttttgatg aaagatccca 961 agaagagatt gggatgtggt ccacgtgatg cagatgaaat caaagaacat ctcttctttc 1021 agaaaataaa ttgggatgat ttagccgcca aaaaagtgcc tgcaccattt aagccagtca 1081 ttcgagatga attagatgtg agtaactttg cagaagagtt cacagaaatg gatcccactt 1141 attctcccgc agccctgccc cagagttctg agaagctgtt tcagggctat tcctttgttg 1201 ctccttccat cctattcaag cgtaatgcag ctgtcataga ccctcttcag tttcacatgg 1261 gagttgaacg tcctGGAGTG ACAAATGTTG CCAGGAGTGC AATGATGAAG GACTCTCCAT 1321 TCTATCAACA CTATGACCTA GATTTGAAGG ACAAACCCCT GGGAGAAGGT AGTTTTTCAA 1381 TTTGTCGAAA GTGTGTGCAT AAAAAAAGTA ACCAAGCTTT TGCAGTCAAA ATAATCAGCA 1441 AAAGGATGGA AGCCAATACT CAAAAGGAAA TAACAGCTCT GAAACTCTGT GAAGGACACC 1501 CCAATATTGT GAAGTTGCAT GAAGTTTTTC ATGATCAGCT TCACACGTTT CTAGTGATGG 1561 AACTTCTGAA TGGAGGAGAA CTGTTTGAGC GCATTAAGAA AAAGAAGCAC TTCAGTGAGA 1621 CGGAAGCCAG CTACATCATG AGGAAGCTTG TTTCAGCTGT AAGCCACATG CATGATGTTG 1681 GAGTGGTGCA CAGGGATCTG AAACCTGAGG TATACCCAAC TTTCTTGTAC AAAGTGGTTG 1741 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1801 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 1861 TTTACTTTAC GTCATCATTA CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 1921 ttgtgaaaga tt