Construct: ORF TRCN0000466446

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002968.3_s317c1
Derived from:
ccsbBroadEn_02123
DNA Barcode:
TCTTTACTTTACGTCATCATTACA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RPS6KA5 (9252)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466446
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_182398.2 100% 100%
2 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322229.2 69% 68.9% 1645_1648delAATTinsG;1651_2385del
3 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_004755.4 68.4% 68.3% 1645_1648delAATTinsG;1651_2406del
4 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322236.2 64.9% 64.8% 618_619ins84;1561_1564delAATTinsG;1567_2322del
5 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322228.2 63.9% 63.8% 510_511ins108;1537_1540delAATTinsG;1543_2298del
6 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322233.2 61.6% 61.5% 951_952ins162;1483_1486delAATTinsG;1489_2244del
7 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322232.2 59.3% 59.2% 173_174ins219;1426_1429delAATTinsG;1432_2187del
8 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322235.2 58.5% 58.4% 0_1ins237;1408_1411delAATTinsG;1414_2169del
9 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322237.2 58.5% 58.4% 0_1ins237;1408_1411delAATTinsG;1414_2169del
10 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322234.2 44.1% 43.3% (many diffs)
11 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322238.2 41.1% 41% 0_1ins657;988_991delAATTinsG;994_1749del
12 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 XM_017021786.2 41.1% 41% 0_1ins657;988_991delAATTinsG;994_1749del
13 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322231.2 38.6% 35.3% (many diffs)
14 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322230.2 35% 34.9% (many diffs)
15 human 9252 RPS6KA5 ribosomal protein S6 kinase A5 NM_001322227.2 33.3% 33.2% 0_1ins843;802_805delAATTinsG;808_1563del
16 mouse 73086 Rps6ka5 ribosomal protein S6 kinase... NM_001330702.1 62.1% 66.5% (many diffs)
17 mouse 73086 Rps6ka5 ribosomal protein S6 kinase... XM_006516275.3 59.5% 63.5% (many diffs)
18 mouse 73086 Rps6ka5 ribosomal protein S6 kinase... NM_153587.3 57.4% 61.5% (many diffs)
19 mouse 73086 Rps6ka5 ribosomal protein S6 kinase... XM_006516276.3 37.1% 39.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1713
ORF length:
1647
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga ggaggagggt ggcagcagcg gcggcgccgc ggggaccagc gcggacggcg
121gcgacggagg agagcagctc ctcactgtca agcacgagct gcggactgct aatttgacag
181gacatgctga gaaggtggga atagaaaatt ttgagctcct gaaggtccta ggaactggag
241cttatggaaa agtatttcta gttcgtaaaa taagtggcca tgatactgga aagctgtatg
301ccatgaaagt tttgaaaaag gcaacaatcg ttcaaaaggc caaaaccaca gagcatacaa
361ggacagaacg acaagtcctg gaacacatta ggcagtcgcc atttttggta acattacatt
421atgctttcca gacagaaacc aaacttcatc tcattttaga ttatataaat ggtggtgaac
481tttttactca tctttctcaa agagagcgtt tcacagagca tgaggtgcag atttatgttg
541gagagattgt gcttgccctc gaacatctcc acaagttggg gattatatat cgtgatatta
601agcttgagaa tattctactt gattctaatg gccatgtggt gctgacagat tttggtctga
661gtaaggagtt tgtggctgat gaaactgaaa gagcatattc cttttgtgga actattgaat
721acatggcacc agatattgtc agagggggag attcaggaca tgacaaggca gttgactggt
781ggagtttggg tgttctaatg tatgaattac taactggagc atctcctttc actgttgatg
841gagaaaaaaa ttcccaagct gagatatcta ggagaatatt aaaaagtgag cctccatatc
901cccaagaaat gagtgcttta gcgaaagacc taattcagcg tcttttgatg aaagatccca
961agaagagatt gggatgtggt ccacgtgatg cagatgaaat caaagaacat ctcttctttc
1021agaaaataaa ttgggatgat ttagccgcca aaaaagtgcc tgcaccattt aagccagtca
1081ttcgagatga attagatgtg agtaactttg cagaagagtt cacagaaatg gatcccactt
1141attctcccgc agccctgccc cagagttctg agaagctgtt tcagggctat tcctttgttg
1201ctccttccat cctattcaag cgtaatgcag ctgtcataga ccctcttcag tttcacatgg
1261gagttgaacg tcctGGAGTG ACAAATGTTG CCAGGAGTGC AATGATGAAG GACTCTCCAT
1321TCTATCAACA CTATGACCTA GATTTGAAGG ACAAACCCCT GGGAGAAGGT AGTTTTTCAA
1381TTTGTCGAAA GTGTGTGCAT AAAAAAAGTA ACCAAGCTTT TGCAGTCAAA ATAATCAGCA
1441AAAGGATGGA AGCCAATACT CAAAAGGAAA TAACAGCTCT GAAACTCTGT GAAGGACACC
1501CCAATATTGT GAAGTTGCAT GAAGTTTTTC ATGATCAGCT TCACACGTTT CTAGTGATGG
1561AACTTCTGAA TGGAGGAGAA CTGTTTGAGC GCATTAAGAA AAAGAAGCAC TTCAGTGAGA
1621CGGAAGCCAG CTACATCATG AGGAAGCTTG TTTCAGCTGT AAGCCACATG CATGATGTTG
1681GAGTGGTGCA CAGGGATCTG AAACCTGAGG TATACCCAAC TTTCTTGTAC AAAGTGGTTG
1741ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1801GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC
1861TTTACTTTAC GTCATCATTA CAACGCGTTA AGTCgacaat caacctctgg attacaaaat
1921ttgtgaaaga tt

Download FASTA (ORF) (Full)