Transcript: Human NM_001322234.2

Homo sapiens ribosomal protein S6 kinase A5 (RPS6KA5), transcript variant 10, mRNA.

Source:
NCBI, updated 2019-06-04
Taxon:
Homo sapiens (human)
Gene:
RPS6KA5 (9252)
Length:
26866
CDS:
821..2653

Additional Resources:

NCBI RefSeq record:
NM_001322234.2
NBCI Gene record:
RPS6KA5 (9252)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146211 GATTTGAAGGACAAACCCCT pXPR_003 GGG 716 39% 11 0.2861 RPS6KA5 RPS6KA5 77371
2 BRDN0001146427 CATGCAACTTCACAATATTG pXPR_003 GGG 863 47% 12 0.0167 RPS6KA5 RPS6KA5 77373
3 BRDN0001145521 GGCACCAGATATTGTCAGAG pXPR_003 GGG 100 5% 6 -0.1293 RPS6KA5 RPS6KA5 77372
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001322234.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000001498 CGCGGTGGAAATCATGAAGAA pLKO.1 2143 CDS 100% 4.950 6.930 N RPS6KA5 n/a
2 TRCN0000195329 CCGGATATTCTAGGATCTTCC pLKO.1 2345 CDS 100% 4.050 5.670 N RPS6KA5 n/a
3 TRCN0000001496 GCAGATTTATGTTGGAGAGAT pLKO.1 669 5UTR 100% 4.950 3.960 N RPS6KA5 n/a
4 TRCN0000001494 ACCTATGACTTGTTTGGAAAT pLKO.1 3714 3UTR 100% 10.800 7.560 N RPS6KA5 n/a
5 TRCN0000196479 GAAGCTTGTTTCAGCTGTAAG pLKO.1 1822 CDS 100% 10.800 7.560 N RPS6KA5 n/a
6 TRCN0000196345 GTGCACTGTTTAAAGCATTTG pLKO.1 3444 3UTR 100% 10.800 7.560 N RPS6KA5 n/a
7 TRCN0000196766 GTTTGGGTGTTCTAATGTATG pLKO.1 963 CDS 100% 10.800 7.560 N RPS6KA5 n/a
8 TRCN0000001497 GCTGAGAAGGTGGGAATAGAA pLKO.1 328 5UTR 100% 5.625 3.938 N RPS6KA5 n/a
9 TRCN0000001495 GCACCATTTAAGCCAGTCATT pLKO.1 1241 CDS 100% 4.950 3.465 N RPS6KA5 n/a
10 TRCN0000379468 AGCAACCTTCCACGCCTTTAA pLKO_005 2392 CDS 100% 13.200 7.920 N RPS6KA5 n/a
11 TRCN0000434454 AGACCAACCTGGGCAACATAG pLKO_005 5482 3UTR 100% 10.800 5.400 Y SULT1A1 n/a
12 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 21792 3UTR 100% 4.950 2.475 Y ORAI2 n/a
13 TRCN0000148774 CCATGTGTTCTCATTGTTCAA pLKO.1 14804 3UTR 100% 4.950 2.475 Y GLIPR1L2 n/a
14 TRCN0000162188 CCATGTGTTCTCATTGTTCAA pLKO.1 14804 3UTR 100% 4.950 2.475 Y SPC25 n/a
15 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 13554 3UTR 100% 4.950 2.475 Y ERAP2 n/a
16 TRCN0000149064 GCAGGTTTGTTACATAGGTAT pLKO.1 14655 3UTR 100% 4.950 2.475 Y GLIPR1L2 n/a
17 TRCN0000155229 GATCAAGACCATCCTGGCTAA pLKO.1 23464 3UTR 100% 4.050 2.025 Y INTS7 n/a
18 TRCN0000138140 GATTGAGACCATCCTGGCTAA pLKO.1 21771 3UTR 100% 4.050 2.025 Y LOC441087 n/a
19 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 13555 3UTR 100% 13.200 6.600 Y LIAS n/a
20 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 13067 3UTR 100% 5.625 2.813 Y KLHL30 n/a
21 TRCN0000009295 GCAGGTTTGTTACATAGGTAA pLKO.1 14655 3UTR 100% 4.950 2.475 Y OR11A1 n/a
22 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 13067 3UTR 100% 5.625 2.813 Y EID2B n/a
23 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 10474 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001322234.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02123 pDONR223 100% 44.1% 43.3% None (many diffs) n/a
2 ccsbBroad304_02123 pLX_304 0% 44.1% 43.3% V5 (many diffs) n/a
3 TRCN0000466446 TCTTTACTTTACGTCATCATTACA pLX_317 26.9% 44.1% 43.3% V5 (many diffs) n/a
4 ccsbBroadEn_14934 pDONR223 0% 44.1% 43.3% None (many diffs) n/a
5 ccsbBroad304_14934 pLX_304 0% 44.1% 43.3% V5 (many diffs) n/a
6 TRCN0000480681 TACACAATAACACTCCCGACAATC pLX_317 26.9% 44.1% 43.3% V5 (many diffs) n/a
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