Construct: ORF TRCN0000466448
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003853.1_s317c1
- Derived from:
- ccsbBroadEn_09645
- DNA Barcode:
- TAGTGGCTGCGCGCGAACTGTACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A52 (147407)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466448
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 147407 | SLC25A52 | solute carrier family 25 me... | NM_001034172.4 | 99.8% | 99.6% | 715C>A |
| 2 | human | 92014 | SLC25A51 | solute carrier family 25 me... | NM_033412.4 | 97.7% | 96.2% | (many diffs) |
| 3 | human | 442229 | SLC25A51P1 | SLC25A51 pseudogene 1 | NR_026540.1 | 51.1% | (many diffs) | |
| 4 | human | 494141 | SLC25A51P4 | SLC25A51 pseudogene 4 | NR_026564.1 | 46.8% | (many diffs) | |
| 5 | human | 494141 | SLC25A51P4 | SLC25A51 pseudogene 4 | NR_026563.1 | 43.2% | (many diffs) | |
| 6 | human | 494141 | SLC25A51P4 | SLC25A51 pseudogene 4 | NR_026541.1 | 38.9% | (many diffs) | |
| 7 | mouse | 230125 | Slc25a51 | solute carrier family 25, m... | NM_001009949.3 | 79.9% | 85.2% | (many diffs) |
| 8 | mouse | 230125 | Slc25a51 | solute carrier family 25, m... | XM_006537835.3 | 79.9% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 960
- ORF length:
- 891
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatagattca gaagctcatg aaaagaggcc accaatactt acatcttcaa 121 aacaagatat atcacctcat attacaaatg ttggtgaaat gaagcattac ttgtgtggct 181 gctgtgcagc cttcaacaac gtcgcaatca catatcccat tcagaaggtc ctctttcggc 241 aacagctgta tggcatcaaa acccgggatg cagtacttca gttgagaagg gatggatttc 301 gaaatttgta tcgtggaatc cttcccccat tgatgcagaa gacaactacg cttgcactta 361 tgtttggtct gtatgaggat ttatcctgcc ttctccggaa gcatgtccgt gctccagagt 421 ttgcaaccca tggcgtggcg gcagtgcttg cagggacagc agaagcaatt ttcactccac 481 tggaaagagt tcagacattg cttcaaaacc acaagcatca tgacaaattt accaacactt 541 atcaggcttt caaggcactg aaatgtcatg gaattggaga gtattatcga ggcttggtgc 601 ccattctttT CCGGAATGGA CTCAGCAATG TCTTGTTTTT CGGCCTTCGA GGTCCCATTA 661 AGGAGCATCT GCCTACCGCA ACGACTCACA GTGCTCATTT GGTCAATGAT TTTATCGGTG 721 GAGGTCTATT GGGTGCCATG TTGGGATTCT TGTGTTTTCC AATTAATGTT GTAAAAACTC 781 GCATACAGTC TCAGATTGGT GGGGAATTTC AGTCTTTCCC CAAGGTTTTC CAAAAAATCT 841 GGCTGGAACG GGACAGAAAA CTGATAAATC TTTTCAGAGG TGCCCATCTG AATTACCATC 901 GGTCCCTCAT CTCTTGGGGC ATAATCAATG CAACTTATGA GTTCTTGTTA AAATTTATAT 961 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1021 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1081 TTTATATATC TTGTGGAAAG GACGATAGTG GCTGCGCGCG AACTGTACTA CGCGTTAAGT 1141 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt