Construct: ORF TRCN0000466482
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018797.1_s317c1
- Derived from:
- ccsbBroadEn_15321
- DNA Barcode:
- CTGGCTGCCAGCGCATTTACCTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- STRADBP1 (389599)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466482
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55437 | STRADB | STE20 related adaptor beta | NM_018571.6 | 91.3% | 32.8% | (many diffs) |
| 2 | human | 55437 | STRADB | STE20 related adaptor beta | NM_001206864.2 | 81.6% | 36.3% | (many diffs) |
| 3 | human | 55437 | STRADB | STE20 related adaptor beta | XM_017004438.1 | 67.3% | 8.1% | (many diffs) |
| 4 | human | 55437 | STRADB | STE20 related adaptor beta | XM_005246669.2 | 59.1% | 47.9% | (many diffs) |
| 5 | mouse | 227154 | Stradb | STE20-related kinase adapto... | NM_172656.5 | 82.6% | 29.8% | (many diffs) |
| 6 | mouse | 227154 | Stradb | STE20-related kinase adapto... | XR_373251.2 | 37.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 606
- ORF length:
- 537
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaaaacaag tctgaaacca gtatccatca atacttggtc gaagagccaa 121 ccctttcctg gtcgcctcca tccactagag ccagtgaagt agtatgttct gccaacgttt 181 ctcactacga gctccaagta gaaataggaa gaggatttga caagttgact tctgtccatc 241 tagcacggca tactcctacg ggaacgctgg taactacaaa aattacaaat ctggaaaacg 301 gcaataaaga acgcctgaaa gttttacaga aagccatgat tctatcccac tttttccggc 361 atcccaatat tacaacttat tggacagttt tcactgttgg cagctggctt tgggttattt 421 ctccatttat ggcctatggt tcagcaagac aactcttgag gacctatttt cctgaaggaa 481 tgagtaaaac tttaataaga aacattctct ttggagcagt gaggaggatt gaactatctc 541 tacccaatat ggctgtattc acaggagaat taaagccagc catatcctca tttctggtga 601 tggcctagtg accctctctg gcctgtccca tctgcatagt ttggttaagc atggacagag 661 gcatagggct gtgtatgatt tcccacagtt cagcacatta gtgcagcagc catggctgag 721 tccagaacta ctgagacagg atttacatgg gtataatgtg aagtcagata tttacggtgt 781 tgggattaca acatgtgaat tagccagtgg gcaggtgcct ttccaggacg tgcatagaac 841 tcagatgctg ttaacagaaa ctgaaaggtc ccccttatag cccattggat atcagtattt 901 tccctcaatc agaatccaaa atgaaaaatt cccggtcagg tgtagactct gggattggag 961 caagtgtgct tgtctccagt ggaactcaca cagtaaatag tgaccgatta cacacaccat 1021 cctcaaaaaC CTTCTCTCCT GCCTTCTTTA GCTGGGTACA GCTGTGTTTG CAACAAGATC 1081 CGGAGAAAAG GCCATCAGCA AGCAGTTTAT TGTCCCATGT TTTCTTCAAA CAGATGAAAG 1141 AAGAAAGCCA GGATTCGGTA CTTTCACTGT TGCCTCCTGC TTATAACAAG CCATCAATAT 1201 CACTGCCTCC GGTGTTACCT TGGACTGAGC CAGAATGTGG TTTTCCTGAT GAAAAAGATT 1261 CATATTGGGA ATTCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1321 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1381 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTGGCTGCCA GCGCATTTAC 1441 CTTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt