Construct: ORF TRCN0000466507
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009620.1_s317c1
- Derived from:
- ccsbBroadEn_03688
- DNA Barcode:
- TTTCTGGTAGCCAAGGGACACTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB5IF (55969)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466507
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55969 | RAB5IF | RAB5 interacting factor | NM_001199534.2 | 99.7% | 99.2% | 407C>T |
| 2 | human | 55969 | RAB5IF | RAB5 interacting factor | NM_018840.5 | 92% | 87.5% | 347_348ins28;379C>T;384_387delTGAC |
| 3 | human | 55969 | RAB5IF | RAB5 interacting factor | NM_001374178.1 | 85.9% | 85.4% | (many diffs) |
| 4 | human | 55969 | RAB5IF | RAB5 interacting factor | NM_199483.3 | 68.3% | 57.1% | (many diffs) |
| 5 | human | 100527943 | TGIF2-RAB5IF | TGIF2-RAB5IF readthrough | NM_001199535.2 | 68.1% | 53.7% | (many diffs) |
| 6 | human | 55969 | RAB5IF | RAB5 interacting factor | NR_026562.4 | 31.6% | (many diffs) | |
| 7 | human | 55969 | RAB5IF | RAB5 interacting factor | NR_164351.1 | 31% | (many diffs) | |
| 8 | human | 55969 | RAB5IF | RAB5 interacting factor | NR_164350.1 | 28.1% | (many diffs) | |
| 9 | mouse | 67388 | 1110008F13Rik | RIKEN cDNA 1110008F13 gene | NM_026124.3 | 85.3% | 86.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 477
- ORF length:
- 411
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cggcgggcgg cggaaggagg agccgccTCA GCCGCAGCTG GCCAACGGGG 121 CCCTCAAAGT CTCCGTCTGG AGTAAGGTGC TGCGGAGCGA CGCGGCCTGG GAGGATAAGG 181 ATGAATTTTT AGATGTGATC TACTGGTTCC GACAGATCAT TGCTGTGGTC CTGGGTGTCA 241 TTTGGGGAGT TTTGCCATTA CGAGGGTTCT TGGGAATAGC AGGATTCTGC CTGATCAATG 301 CAGGAGTCCT GTACCTCTAC TTCAGCAATT ACCTACAGAT TGATGAGGAA GAATATGGTG 361 GCACGTGGGA GCTCACGAAG GAAGGGTTTA TGACCTCTTT TGCCTTGTTC ATGGTATGTG 421 TAGCTGATAG TTTTACAACA GGTCATTTGG ATCATCTTTT ACACTGCCAT CTATTATGCC 481 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 541 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 601 ATATATCTTG TGGAAAGGAC GATTTCTGGT AGCCAAGGGA CACTAGACGC GTTAAGTCga 661 caatcaacct ctggattaca aaatttgtga aagatt