Construct: ORF TRCN0000466560
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015096.1_s317c1
- Derived from:
- ccsbBroadEn_11335
- DNA Barcode:
- AATTGTCACGAACTTAATCGTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LATS1 (9113)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466560
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_017011482.1 | 19% | 17.4% | (many diffs) |
| 2 | human | 9113 | LATS1 | large tumor suppressor kina... | NM_001270519.1 | 18.5% | 17% | (many diffs) |
| 3 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_006715603.3 | 14.4% | 13.2% | (many diffs) |
| 4 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_017011479.1 | 12.9% | 11.8% | (many diffs) |
| 5 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_011536252.2 | 12.8% | 11.7% | (many diffs) |
| 6 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_017011477.1 | 12.4% | 11.3% | (many diffs) |
| 7 | human | 9113 | LATS1 | large tumor suppressor kina... | NM_004690.4 | 11.3% | 10.4% | (many diffs) |
| 8 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_017011474.1 | 11% | 10.1% | (many diffs) |
| 9 | human | 9113 | LATS1 | large tumor suppressor kina... | XM_024446583.1 | 11% | 10.1% | (many diffs) |
| 10 | human | 9113 | LATS1 | large tumor suppressor kina... | NR_073033.1 | 5.4% | 1_359del;765_7439del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 471
- ORF length:
- 405
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaggagtgaa aagccagaag gatatagaca aatgaggcct aagaCCTTTC 121 CTGCCAGTAA CTATACTGTC AGTAGCCGGC AAATGTTACA AGAAATTCGG GAATCCCTTA 181 GGAATTTATC TAAACCATCT GATGCTGCTA AGGCTGAGCA TAACATGAGT AAAATGTCAA 241 CCGAAGATCC TCGACAAGTC AGAAATCCAC CCAAATTTGG GACGCATCAT AAAGCCTTGC 301 AGGAAATTCG AAACTCTCTG CTTCCATTTG CAAATGAAAC AAATTCTTCT CGGAGTACTT 361 CAGAAGTTAA TCCACAAATG CTTCAAGACT TGCAAGCTGC TGGATTTGAT GAGGAGGATC 421 ATCTGTCAGT AGCCTGTTCA CCCATTAGTC TTACTAAGCC ATTTCTCATT TACCCAACTT 481 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 541 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 601 CTTGTGGAAA GGACGAAATT GTCACGAACT TAATCGTTTC ACGCGTTAAG TCgacaatca 661 acctctggat tacaaaattt gtgaaagatt