Construct: ORF TRCN0000466600
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015339.1_s317c1
- Derived from:
- ccsbBroadEn_10240
- DNA Barcode:
- GACAATGAGAGATCCGTACAATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CROT (54677)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466600
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54677 | CROT | carnitine O-octanoyltransfe... | NM_001243745.2 | 100% | 100% | |
| 2 | human | 54677 | CROT | carnitine O-octanoyltransfe... | XM_011516337.3 | 14.6% | 13.7% | (many diffs) |
| 3 | human | 54677 | CROT | carnitine O-octanoyltransfe... | NM_021151.4 | 13.9% | 13.1% | (many diffs) |
| 4 | human | 54677 | CROT | carnitine O-octanoyltransfe... | NM_001143935.2 | 13.3% | 12.5% | (many diffs) |
| 5 | mouse | 74114 | Crot | carnitine O-octanoyltransfe... | NM_023733.3 | 12.4% | 11.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 351
- ORF length:
- 261
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGAAAATCA ATTGGCTAAA TCAACTGAAG 121 AACGAACATT TCAGTACCAG GATTCTCTTC CATCACTGCC TGTTCCTTCA CTTGAAGAAT 181 CATTAAAAAA ATACCTTGAA TCAGTGAAAC CATTTGCAAA TCAAGAAGAA TATAAGAAAA 241 CTGAAGAAAT AGTTCAAAAA TTTCAAAGTG GGATTGGAGA AAAATTGCAC CAGAAATTGC 301 TTGAAAGAGC AAAAGGAAAA AGAAATTGGG TATTTGTTGT TATAATTGAA TACCCAACTT 361 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 421 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 481 CTTGTGGAAA GGACGAGACA ATGAGAGATC CGTACAATTC ACGCGTTAAG TCgacaatca 541 acctctggat tacaaaattt gtgaaagatt