Construct: ORF TRCN0000466667
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005338.1_s317c1
- Derived from:
- ccsbBroadEn_07227
- DNA Barcode:
- ACAGCTATGCTGGGTCCGATAGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H4C8 (8365)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466667
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8365 | H4C8 | H4 clustered histone 8 | NM_003543.3 | 99.6% | 99% | 308G>A |
| 2 | human | 8367 | H4C5 | H4 clustered histone 5 | NM_003545.3 | 88.6% | 99% | (many diffs) |
| 3 | human | 8360 | H4C4 | H4 clustered histone 4 | NM_003539.3 | 83.4% | 99% | (many diffs) |
| 4 | human | 8361 | H4C6 | H4 clustered histone 6 | NM_003540.3 | 83.1% | 99% | (many diffs) |
| 5 | human | 8362 | H4C12 | H4 clustered histone 12 | NM_003541.2 | 83.1% | 99% | (many diffs) |
| 6 | human | 8363 | H4C11 | H4 clustered histone 11 | NM_021968.3 | 83.1% | 99% | (many diffs) |
| 7 | human | 8359 | H4C1 | H4 clustered histone 1 | NM_003538.3 | 83% | 99% | (many diffs) |
| 8 | human | 554313 | H4C15 | H4 clustered histone 15 | NM_001034077.4 | 82.8% | 99% | (many diffs) |
| 9 | human | 8294 | H4C9 | H4 clustered histone 9 | NM_003495.2 | 82.8% | 99% | (many diffs) |
| 10 | human | 8370 | H4C14 | H4 clustered histone 14 | NM_003548.2 | 82.8% | 99% | (many diffs) |
| 11 | human | 8366 | H4C2 | H4 clustered histone 2 | NM_003544.2 | 82.3% | 99% | (many diffs) |
| 12 | human | 8364 | H4C3 | H4 clustered histone 3 | NM_003542.3 | 81.2% | 99% | (many diffs) |
| 13 | human | 121504 | H4-16 | H4 histone 16 | NM_175054.2 | 80.1% | 99% | (many diffs) |
| 14 | mouse | 97122 | Hist2h4 | histone cluster 2, H4 | NM_033596.3 | 83.4% | 99% | (many diffs) |
| 15 | mouse | 319156 | Hist1h4d | histone cluster 1, H4d | NM_175654.2 | 83.4% | 99% | (many diffs) |
| 16 | mouse | 100041230 | Hist1h4m | histone cluster 1, H4m | NM_001195421.1 | 83.1% | 99% | (many diffs) |
| 17 | mouse | 319161 | Hist1h4n | histone cluster 1, H4n | NM_175657.2 | 83.1% | 99% | (many diffs) |
| 18 | mouse | 326619 | Hist1h4a | histone cluster 1, H4a | NM_178192.2 | 82.8% | 99% | (many diffs) |
| 19 | mouse | 319159 | Hist1h4j | histone cluster 1, H4j | NM_178210.2 | 82.8% | 99% | (many diffs) |
| 20 | mouse | 319160 | Hist1h4k | histone cluster 1, H4k | NM_178211.2 | 82.8% | 99% | (many diffs) |
| 21 | mouse | 319155 | Hist1h4c | histone cluster 1, H4c | NM_178208.2 | 82.4% | 99% | (many diffs) |
| 22 | mouse | 319157 | Hist1h4f | histone cluster 1, H4f | NM_175655.2 | 82% | 99% | (many diffs) |
| 23 | mouse | 326620 | Hist1h4b | histone cluster 1, H4b | NM_178193.2 | 82% | 99% | (many diffs) |
| 24 | mouse | 319158 | Hist1h4i | histone cluster 1, H4i | NM_175656.3 | 81.4% | 99% | (many diffs) |
| 25 | mouse | 320332 | Hist4h4 | histone cluster 4, H4 | NM_175652.3 | 81.2% | 99% | (many diffs) |
| 26 | mouse | 69386 | Hist1h4h | histone cluster 1, H4h | NM_153173.4 | 81% | 99% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 375
- ORF length:
- 309
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tggccgtggt aaaggtggaa aaggtttggg taagggagga gctaagcgtc 121 atcgcaaggt tttgcgcgat aacatccagg gcatcacTAA GCCAGCTATC CGGCGCCTTG 181 CTCGTCGCGG CGGTGTCAAG CGAATTTCTG GCCTTATCTA TGAGGAGACT CGTGGTGTTC 241 TGAAGGTGTT CCTGGAGAAC GTGATTCGTG ACGCTGTCAC TTACACAGAG CACGCCAAAC 301 GCAAGACCGT GACAGCAATG GATGTGGTCT ACGCGCTGAA GCGACAGGGA CGCACTCTTT 361 ACGGCTTCGG TGACTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 421 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 481 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACAGCTATGC TGGGTCCGAT 541 AGTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt