Construct: ORF TRCN0000466675

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013696.1_s317c1
Derived from:
BRDN0000394779
DNA Barcode:
GTACCCCAGTCATAGTGCGCCCTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ARHGAP27 (201176)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466675
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 201176 ARHGAP27 Rho GTPase activating prote... NM_199282.3 99.7% 99.4% 14T>C;16_17delAGinsCC;932C>G
2 human 201176 ARHGAP27 Rho GTPase activating prote... XM_011524474.1 94% 93.8% (many diffs)
3 human 201176 ARHGAP27 Rho GTPase activating prote... XM_017024312.1 79.3% 79.1% (many diffs)
4 human 201176 ARHGAP27 Rho GTPase activating prote... XM_005257128.3 76.1% 75.9% (many diffs)
5 human 201176 ARHGAP27 Rho GTPase activating prote... XM_011524472.2 75.7% 75.4% (many diffs)
6 human 201176 ARHGAP27 Rho GTPase activating prote... XM_011524471.1 62.6% 62% (many diffs)
7 human 201176 ARHGAP27 Rho GTPase activating prote... NM_001282290.1 61.4% 61.3% (many diffs)
8 human 201176 ARHGAP27 Rho GTPase activating prote... XM_006721745.2 61.4% 61.3% (many diffs)
9 human 201176 ARHGAP27 Rho GTPase activating prote... XM_011524468.1 59.2% 59.1% (many diffs)
10 human 201176 ARHGAP27 Rho GTPase activating prote... XM_017024311.1 59.2% 59.1% (many diffs)
11 human 201176 ARHGAP27 Rho GTPase activating prote... XM_006721747.2 59% 58.8% (many diffs)
12 human 201176 ARHGAP27 Rho GTPase activating prote... XM_011524469.1 56.9% 56.7% (many diffs)
13 human 201176 ARHGAP27 Rho GTPase activating prote... XR_001752447.1 53.2% (many diffs)
14 human 201176 ARHGAP27 Rho GTPase activating prote... XR_429876.3 52.9% (many diffs)
15 human 201176 ARHGAP27 Rho GTPase activating prote... XM_011524470.2 52.7% 49.6% (many diffs)
16 human 201176 ARHGAP27 Rho GTPase activating prote... XR_429873.3 52.5% (many diffs)
17 human 201176 ARHGAP27 Rho GTPase activating prote... XR_001752448.1 52.3% (many diffs)
18 human 201176 ARHGAP27 Rho GTPase activating prote... XR_001752446.1 51.5% (many diffs)
19 human 201176 ARHGAP27 Rho GTPase activating prote... XR_429875.3 48.5% (many diffs)
20 human 201176 ARHGAP27 Rho GTPase activating prote... XR_934416.2 35.2% (many diffs)
21 human 201176 ARHGAP27 Rho GTPase activating prote... XR_934417.3 35.1% (many diffs)
22 human 201176 ARHGAP27 Rho GTPase activating prote... XR_002957982.1 33.2% (many diffs)
23 human 201176 ARHGAP27 Rho GTPase activating prote... XM_017024313.1 32% 30.4% (many diffs)
24 human 201176 ARHGAP27 Rho GTPase activating prote... XM_006721750.4 30.7% 30.4% (many diffs)
25 human 201176 ARHGAP27 Rho GTPase activating prote... XM_011524473.2 27.3% 26.8% (many diffs)
26 mouse 544817 Arhgap27 Rho GTPase activating prote... NM_133715.5 64.7% 66.2% (many diffs)
27 mouse 544817 Arhgap27 Rho GTPase activating prote... XM_006533754.3 63.4% 64.9% (many diffs)
28 mouse 544817 Arhgap27 Rho GTPase activating prote... NM_001205236.1 50.3% 51.5% (many diffs)
29 mouse 544817 Arhgap27 Rho GTPase activating prote... XR_001780030.1 28.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1713
ORF length:
1644
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gcagccgggc ccgccccctg gcagcccagg ggacccgcgg ccccccactc
121ccgagacgga ctaccccgag tcgctgacca gttaccccga ggaggactat tctcccgtgg
181gctctttcgg tgagcccggc cctacctctc ccttgaccac accccccggc tggtcttgtc
241atgtcagcca ggacaagcag atgctctaca ccaaccactt cactcaggag cagtgggtga
301ggctggagga cccccacggg aagccatact tctacaatcc agaggactcc tctgttcgat
361gggagctgcc ccaggtccct gtccctgccc ctcgaagcat ccataaatcc agccaggatg
421gtgacacccc agcccaggcc agccctccag aggagaaggt cccagcagag ctggatgagg
481ttgggagctg ggaggaagtc tctcctgcca cagctgctgt gaggaccaag accttggaca
541aggcaggggt gctccatcgc accaagacgg cagacaaggg aaagcggctc cggaagaagc
601actggagtgc ctcctggact gtgctggagg gtggcgtcct gacattcttc aaggactcaa
661agacctcggc tgcaggcggc ctgaggcagc cttccaagtt ttccacccct gagtacacag
721tggagctgag gggggccact ctctcctggg cccccaaaga caaatccagt aggaagaatg
781tgctggagct acggagccga gatggctctg agtacctgat ccagcacgac tcggaggcca
841tcatcagcac ctggcataag gccattgctc agggcatcca ggagctgtcc gcagagctgc
901ccccagagga gagcgagagc agcagagtgg acttcgggtc gagcgagcgc ttgggaagct
961ggcaggagaa agaggaggac gcgcgaccga atgcagccgg gcccgccctg ggccccgtgg
1021gcctggagag cgacttgagc aaggtccggc acaagctccg caagttcctc cagaggcggc
1081ccacactgca gtcgctgcgg gagaagggct acatcaaaga ccaggtgttc ggctgcgcgc
1141tggccgcgct gtgtgagcgc gagaggagcc gggtgccacg cttcgtgcag cagtgcatcc
1201gcgccgtcga ggcccgcggg ctggacatcg acgggctgta ccgcatcagt ggaaacctgg
1261ccaccatcca gaagctacgc tataaggtgg accacgatga gcgccttgac ctggatgacg
1321ggcgctggga ggacgtccac gttatcaccG GAGCCCTGAA GCTCTTCTTT CGGGAGCTGC
1381CCGAGCCCCT CTTCCCCTTC TCGCACTTCC GCCAGTTCAT TGCGGCCATC AAGTTGCAGG
1441ACCAGGCCCG GCGCAGCCGC TGTGTGCGTG ACTTGGTGCG CTCGCTGCCC GCTCCCAACC
1501ACGACACTCT GCGGATGCTC TTCCAGCACC TCTGCCGGGT GATCGAGCAC GGCGAGCAGA
1561ACCGCATGTC GGTGCAGAGC GTGGCCATTG TGTTCGGGCC CACGCTGCTG CGGCCCGAGG
1621TGGAAGAGAC CAGCATGCCC ATGACCATGG TGTTCCAGAA CCAGGTGGTG GAGCTCATCC
1681TGCAGCAGTG CGCGGACATC TTCCCGCCGC ACTTGCCAAC TTTCTTGTAC AAAGTGGTTG
1741ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1801GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGT
1861ACCCCAGTCA TAGTGCGCCC TTACGCGTTA AGTCgacaat caacctctgg attacaaaat
1921ttgtgaaaga tt

Download FASTA (ORF) (Full)