Construct: ORF TRCN0000466703
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012267.1_s317c1
- Derived from:
- ccsbBroadEn_04884
- DNA Barcode:
- ACAAATACCTATTAGCCAATGAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NADK2 (133686)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466703
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 133686 | NADK2 | NAD kinase 2, mitochondrial | NM_001287340.1 | 100% | 100% | |
| 2 | human | 133686 | NADK2 | NAD kinase 2, mitochondrial | NM_153013.4 | 100% | 100% | |
| 3 | human | 133686 | NADK2 | NAD kinase 2, mitochondrial | NM_001287341.1 | 92.6% | 92.6% | 372_437del |
| 4 | human | 133686 | NADK2 | NAD kinase 2, mitochondrial | XM_024454360.1 | 92.6% | 92.6% | 372_437del |
| 5 | human | 133686 | NADK2 | NAD kinase 2, mitochondrial | NM_001085411.3 | 63.1% | 63.1% | 1_489del |
| 6 | human | 133686 | NADK2 | NAD kinase 2, mitochondrial | XM_005248241.4 | 60.1% | 60.1% | 1_489del;861_926del |
| 7 | mouse | 68646 | Nadk2 | NAD kinase 2, mitochondrial | NM_001040395.4 | 57.9% | 61.8% | (many diffs) |
| 8 | mouse | 68646 | Nadk2 | NAD kinase 2, mitochondrial | NM_001085410.2 | 55.1% | 58.8% | (many diffs) |
| 9 | mouse | 68646 | Nadk2 | NAD kinase 2, mitochondrial | NM_001286255.1 | 52.4% | 56% | (many diffs) |
| 10 | mouse | 68646 | Nadk2 | NAD kinase 2, mitochondrial | NM_001286253.1 | 48.3% | 51.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 903
- ORF length:
- 837
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gctggcagcg agtaaagtct tggacagact taaaccagtt ataggggtaa 121 acactgatcc agaacggtct gagggtcatt tatgcctgcc cgttcgatat acacattcct 181 ttccagaagc cttacagaag ttctatcgtg gtgagttcag gtggttgtgg aggcagagaa 241 tcaggttata ccttgaaggg actggcataa accctgtacc tgtggacctt cacgagcagc 301 agctaagctt gaatcagcac aatagagccc ttaacattga aagagctcat gatgaaaggt 361 ctgaggcttc aggaccccaa cttctgccag tgagagcact aaatgaagtc ttcattgggg 421 agagtctgtc atccagggct tcctactatg agatttcagt tgatgatggt ccatgggaaA 481 AACAGAAGAG TTCAGGGCTC AATTTGTGTA CTGGAACAGG ATCAAAGGCC TGGTCATTCA 541 ATATTAACAG GGTTGCAACT CAGGCTGTAG AAGATGTTTT AAATATTGCA AAACGACAAG 601 GAAATTTGAG TCTTCCATTG AACAGAGAAT TGGTAGAGAA AGTAACAAAT GAATATAATG 661 AATCACTGCT CTACAGTCCG GAAGAACCAA AAATACTTTT CAGTATTCGA GAACCAATAG 721 CAAATAGAGT TTTCTCAAGC AGTCGTCAGC GTTGTTTCTC CTCAAAGGTT TGTGTTCGTT 781 CTCGTTGTTG GGATGCCTGT ATGGTTGTGG ATGGAGGAAC TTCTTTTGAG TTTAATGATG 841 GTGCAATTGC TTCGATGATG ATCAATAAAG AAGATGAGCT TCGAACTGTG CTTCTTGAAC 901 AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 961 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1021 GGCTTTATAT ATCTTGTGGA AAGGACGAAC AAATACCTAT TAGCCAATGA GAACGCGTTA 1081 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt